Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Scavenging Activities of Reactive Oxygen and Nitrogen Species by Junglans sinensis

호도(胡桃)의 활성산소 및 활성질소 제거 기전

  • Jeong Ji-Cheon (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Bae Sung-Min (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Shin Hyeon-Cheol (Department of Oriental Internal Medicine, College of Oriental Medicine, Daegu Hanny University)
  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 배성민 (동국대학교 한의과대학 내과학교실) ;
  • 신현철 (대구한의대학교 한의과대학 내과학교실)
  • Published : 2005.12.01
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Abstract

Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are widely implicated in the aging process and age-related diseases. The present study was carried out to investigate scavenging activities of Junglans sinensis extract and its subfraction using fluorescent probes, DCF-DA, DAF-2 and DHR 123. Jungians sinensis was washed and crushed. The crushed Junglans sinensis was extracted 3times, each time with 3 volumes of methyl alcohol at $60^{\circ}C$ for 24 h. The extract was filtered and evaporated under a reduced pressure using a rotary evaporator to yield 16 g. Scavenging activities of $ONOO^-$ was measured by Kooy' method and ROS was measured by DCFDA assay. Junglans sinensis had the marked scavenging activites of $ONOO^-$, NO and $O_2^-$. Junglans sinensis scavenged $ONOO^-$ through electron donation and dose-dependently inhibited the nitration of bovine serum albumin by $ONOO^-$. Junglans sinensis also had ROS scavenging activity. Especially, ethylacetate fraction of Junglans sinensis showed the most effective scavenging activities for ROS and RNS. These results suggest that Junglans sinensis might be developed as an effective ROS and RNS scavenger Therefore, Junglans sinensis might be used as a preventive agent for the aging and relevant to aging of illness.

Keywords

References

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