Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Effects of Cadmium on Glucose Transport in 3T3- L1 adipocytes

3T3-L1 지방세포주에서 포도당 수송에 미치는 $CdCl_2$의 영향

  • Kang Donghee (College of Pharmacy, Seoul National University) ;
  • Khil Lee-Yong (Lab of Viral and Immunopathogenesis of Diabetes, Julia McFarlane Diabdetes Research Center and Dep. of MID, Faculty of Medicine, University of Calgary) ;
  • park Kwangsik (College of Pharmacy, Dongduk Women's University) ;
  • Lee Byung-Hoon (College of Pharmacy, Seoul National University) ;
  • Moon Chang- Kiu (College of Pharmacy, Seoul National University)
  • Published : 2005.03.01
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Abstract

Cadmium is well known as a toxic metal and has insulin mimicking effects in rat adipose tissue. This study was undertaken to investigate the effect of CdCl₂ on glucose transport and its mechanism in 3T3 - L1 adipocytes. CdCl₂ exhibits respectively 2.2 and 2.8 fold increases in the 2-deoxyglucose uptake when exposed to 10 and 25 μM of CdCl₂ for 12 hr. To investigate the stimulating mechanism of glucose transport induced by CdCl₂. Wortmannin and PD98059 were used respectively as PI3K inhibitor and MAPK inhibitor, which did not affect 2-DOG uptake. This results suggest that induced 2-deoxy-(l-3H)-D-glucose (2-DOG) uptake by CdCl₂ may not be concerned with the insulin signalling pathway. Whereas nifedipine, a calcium channel blocker inhibited the 2- DOG uptake stimulated by CdCl₂. In addition, we also measured the increased production of Reactive oxygen substances (ROS) and glutathione (GSH) level in 3T3-L1 adipocytes to investigate correlation between the glucose uptake and increased production of ROS with H2DCFDA. CdCl₂ increased production of ROS. Induced 2-DOG uptake and increased production of ROS by CdCl₂ were decreased by N-acetylcystein (NAC). And L-buthionine sulfoximine (BSO) a potent inhibitor of γ-GCS, decreased of 2-DOG uptake. Also NAC and BSO changed the cellular GSH level, but GSH/GSSG ratio remained unchanged at 10, 25 μM of CdCl₂.

Keywords

References

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