Studies on Nosocomial Pathogens Isolated from Veterinary Hospitals in Gwangju

광주지역 동물병원에서 분리한 의원성 병원체에 관한 연구

  • Kang Mi-young (College of Veterinary Medicine, Chonnam National University) ;
  • Choi Jae-won (College of Veterinary Medicine, Chonnam National University) ;
  • Park Seo-jung (College of Veterinary Medicine, Chonnam National University) ;
  • Koh Hong-bum (College of Veterinary Medicine, Chonnam National University) ;
  • Lee Bong-joo (College of Veterinary Medicine, Chonnam National University)
  • 강미영 (전남대학교 수의과대학 수의학과) ;
  • 최재원 (전남대학교 수의과대학 수의학과) ;
  • 박서정 (전남대학교 수의과대학 수의학과) ;
  • 고홍범 (전남대학교 수의과대학 수의학과) ;
  • 이봉주 (전남대학교 수의과대학 수의학과)
  • Published : 2005.03.01

Abstract

We investigated the contamination of animal hospital floor, beauty table, computer keyboard, exam table, operation table and forcep handle by isolations of aerobic bacteria in small animal hospitals in Gwangju. The total number of aerobic bacteria was 52 isolates and Staphylococcus spp. (38 isolates) were the predominant isolates (69.71 %) of them. The prevalent contaminated areas were floor (17 isolates), beauty table (13 isolates) and computer keyboard (9 isolates). The detection of methicillin-resistant (mecA) gene, determined by PCR, showed that 3 of the 17 coagulase-negative Staphylococcus spp. (CNS) isolates possessed the mecA gene. For evaluating the antibiotic susceptibility patterns of the isolates, disk diffusion method was used. The majority of isolates showed high susceptibility to amoxicillin (92.1 %), ceftiofur (84.2%) and polymixin B (73.7%). Also they showed the high resistant to ampicilline (66.7%), penicillin (65%) and kanamycin (56.5%). These results suggest extensive contamination of aerobic bacteria in animal hospital environment.

Keywords

References

  1. Abramson MA, Sexton DJ. Nosocomial methicillin-resistant and methicillin-susceptible Staphylococcus aureus primary bacteremia: at what costs? Infect Control Hosp Epidemiol 1999; 20: 408-11 https://doi.org/10.1086/501641
  2. Bauer AW, Kirby WMM, Sherris TC. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 1999; 4: 493-496
  3. Burnie JO, Naderi-Nsab M, Loudon KW, Matthews RC. An epidemiological study of blood culture isolates of coagulase-negative staphylococci demonstrating hospital-acquired infection. J Clin Micobiol 1997; 35: 1746-1750
  4. Boerlin P, Eugster S, Gaschen F, Straub R, Schawalder P. Transmission of opportunistic pathogens in a veterinary teaching hospital. Vet Micobiol 2001; 82: 347-359
  5. Del'Alamo L, Cereda RF, Tosin I, Mirnda EA, Sader HS. Anitimicrobial susceptibility of coagulase-negative staphylococci and characterization of isolates with reduced susceptibility to glycopeptides. Diagn Microbiol Infect Dis 1999; 34: 185-191 https://doi.org/10.1016/S0732-8893(99)00034-6
  6. Enover FC, Gaynes RP. The epidemiology of Staphylococcus infections. In: Gram-positive Pathotgens, Washington, DC: American Society for Microbiology. 2000: 414-421
  7. Fox JG, Beaucage CM, Folta CA, Thornton GW. Nosocomial transmission of Serratia marcescens in a veterinary hospital due to contamination by benzalkonium chloride. J Clin Microbiol 1981; 14: 157-160
  8. Francey T, Gaschen F, Nicolet J, Burnens AP. The role of Acinetobacter baumannii as a mosocomial pathogen for dogs and cats in an intensive care unit. J Vet Int Med 2000; 14: 177-183 https://doi.org/10.1892/0891-6640(2000)014<0177:TROBAA>2.3.CO;2
  9. Glickman LT. Veterinary nosocomial (hospital-acquired) Klebsiella infections. J am Vet Met Med Assoc 1981; 179: 1398-1392
  10. Kloos WE, Bannerman TL. Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Reiews 1994; 7: 117-140
  11. LaForce FM. The control of Infections in hospitals : 1750-1950. In: Prevention and control of Nosocomial infections, Baltimore: Williams & Wilkins. 1997: 3-17
  12. MacFaddin JF. Biochemical tests for identification of medical bacteria, Waverly Press, Inc. 2nd ed. 1980: 371-438
  13. Moller JK. Monitoring antimicrobial drug resistance in hospital microorganisms. Definition of problems and methods. Dan Med Bull 1990 Jun; 37: 263-274
  14. Oie S, Hosokawa I, Kamiya A. Contamination of room door handles by methicillin-sensitive/methicillin-resistant Staphylococcus aureus. J Hosp Infect 2002; 51: 140-143 https://doi.org/10.1053/jhin.2002.1221
  15. Rupp ME, Archer GL. Coagulase-negative staphylococci: athogens associated with medical progress. Clin Infect Dis 1994; 19: 231-245 https://doi.org/10.1093/clinids/19.2.231
  16. Santos KRN, Fonseca LS, Neto GP, Gontijo Filho PP. Surgical site infection: rates, etiology and resistance patterns to antimicrobials among strains isolated at Rio de Janeiro University Hospital. Infect 1997; 25: 217-220 https://doi.org/10.1007/BF01713147
  17. The Hospital Infection Control Practices Advisory Committee. Guideline for prevention of surgical site infection, 1999. Infect Control Hosp Epidemiol 1999; 20: 247-278
  18. Tuazan CU, Miller H. Clinical and microbiological aspects of serious infections caused by Staphylococcus epidermidis. Scand G infect Dis 1983; 15: 347-60
  19. Vannuffel PJ, Gigi H, Ezzedine B. Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol 1995; 33: 2864-2867
  20. Wendt C. Herwaldt LA, Epidemics : identification and management. In: Prevention and Control of Nosocomial infections, Baltimore: Williams & Wilkins. 1997; 175-213
  21. Wong ES. Surgical site infections. In: Hospital Epidemiology and infection control. Baltimore: Williams & Wilkins. 1996: 154-175
  22. 대한병원감염관리학회 병원감염률 조사위원회. 1996년도 국내 병원감염률 조사연구. 병원감염관리 1997; 2: 157-176
  23. 대한병원감염관리학회 보건의료기술 연구개발팀. 수술부위 감염이 의료비용 및 재원기간에 미치는 영향에 대한 연구. 병원감염관리 1999; 4: 193-204