Propolis 투여가 Streptozotocin으로 유발된 당뇨병 Rat의 혈당에 미치는 영향

Hypoglycemic Effects of Propolis to the Streptozotocin Induced Diabetic Rats

  • 정년기 (충남대학교대학원 보건학과) ;
  • 이동배 (충남대학교 의과대학 예방의학교실) ;
  • 조영채 (충남대학교 의과대학 예방의학교실) ;
  • 하창수 (충남대학교 의과대학 예방의학교실)
  • Chung, Nyun-Ki (Department of Public Health, Graduate School of Chungnam National University) ;
  • Lee, Dong-Bae (Department of Preventive Medicine and Public Health, College of Medicine, Chungnam National University) ;
  • Cho, Young-Chae (Department of Preventive Medicine and Public Health, College of Medicine, Chungnam National University) ;
  • Ha, Chang-Su (Department of Preventive Medicine and Public Health, College of Medicine, Chungnam National University)
  • 발행 : 2005.03.01

초록

To evaluate the hypoglycemic effect of propolis, streptozotocin (STZ) induced diabetic rats were divided into 4 groups such as, diabetic control group, low dose of propolis (0.1 ml) group, medium dose of propolis (0.3 ml) group and high dose of propolis (0.9 ml) group and feeded with propolis extracts for 30 days. After experiment, oral glucose tolerance test (Oral GTT) was carried, and 16 hours fasting blood sugar levels, body weights, blood lipid levels were measured. Finally, pancreatic histopathological study was performed. In conclusion, the propolis is effective to the treatment diabetes due to the reduction of the blood sugar level and the regeneration of the damaged $\beta$-cells shown in streptozotocininduced diabetic rats.

키워드

참고문헌

  1. Monti, M., Berti, E., Carminati, G. and Cusini, M. : Occupational and cosmetic dermatitis from propolis. Contact Dermatitis, 9, 163-164, 1983
  2. Hausen, B. M., Wollenweber, E., Senff, H. and Post, B. : Propolis allergy. Contact Dermatitis, 17, 163-170, 1987 https://doi.org/10.1111/j.1600-0536.1987.tb02699.x
  3. Walker, P. and Crane, E. : Constituents of propolis. Apidologie. 18(4), 327-334, 1987 https://doi.org/10.1051/apido:19870404
  4. Grunberger, D., Banerjee, R, Eisinger, K., Oltz, E. M., Efros, L., Caldwell, M., Estevez, V. and Nakanishi, K. : Prepferential cytotoxicity on tumor cells by caffeic acid phenetyl ester isolated from propolis. Experientia, 44(3), 230-232, 1988 https://doi.org/10.1007/BF01941717
  5. Grange, J. M. and Davey, R. Q. : Antibacterial properties of propolis (bee glue). J Royal Society of Medicine, 83(3), 159-160, 1990 https://doi.org/10.1177/014107689008300310
  6. Serkedjieva, J. and Manolova, N. : Anti-influenza virus effect of propolis constituents and their analogues(esters of substituted cinamic acid). J. Nat Products, 55, 294-297, 1992 https://doi.org/10.1021/np50081a003
  7. Amoros, M. and Simoes L. Girre : Synergistic effect of flavonoids and flavonols against herpes simplex virus type I in cell culture. composition with the antiviral activity of propolis. J. Nat Prod, 55, 732-742, 1992
  8. Rafael, O. P., Niraldo, P., Celio, H. M., Amarilis, S. and Joao, B. C. : Anti-hyperalgesic effect of an ethanolic extract of propolis in mice and rat. Journal of Pharmacy and Pharmacology, 50, 1187-1193, 1998 https://doi.org/10.1111/j.2042-7158.1998.tb03333.x
  9. 신윤진 : 프로폴리스의 항염증 효과에 대한 약리학적 평가. 숙명여자대학교 대학원, 석사학위논문, 1994
  10. 김은주 : Propolis 추출물의 항위염 및 항궤양 작용과 유효성분에 관한 연구. 덕성여자대학교 대학원, 박사학위논문, 1997
  11. Magro-Filho O. de AC. Carvalho: Topical effect of propolis in the repair of sulcoplastices by the modifled kazanjian technique. Cytological and clinical evaluation. Journal of Nihon University School of Dentistry, 36(2), 102-111, 1994 https://doi.org/10.2334/josnusd1959.36.102
  12. Beukelman CJ, De Vries PJF, Schaafsma A, Quaries van Ufford HC. Keuenen J. Kroes BH. Van den Worm E. van den Berg AJJ. Labadie RP. Van Dijk : Immunomodulating properties of propolis. Pharm Pharmacol Lett., 7(2//3), 75-77, 1997
  13. Yamauchi, R, Kato, K., Odia, S., Kanaeda, J. and Ueno, Y. : Benzyl caffeate, an antioxidative compound isolated from propolis. Biosci Biotech Biochem, 56(8), 1321-1322, 1992 https://doi.org/10.1271/bbb.56.1321
  14. 송효남 : 아까시 나무와 밤나무 유래 propolis의 특성과 항산화 및 항균 활성. 서울대학교 대학원, 박사학위논문, 1999
  15. Erben-Russ, M., Bors, W. and Saran, M. : Reaction of linoleic and peroxy. radicals with phenolic antioxidants-apulse radiolysis study international. Journal of Radiaction Biology, 52, 393-412, 1987 https://doi.org/10.1080/09553008714551871
  16. Matsui, T., Ebuchi, S., Fujise, T., Kanthi, J. M., Abesundara, S. D., Yamada, H. and Matsumoto, K. : Strong antihyperglycemic effects of water-soluble fraction of Brazilian propolis and its bioactive constituent, 3,4.5-tri-o-caffeoylquininc acid. Bioi pharm bull, 27(11), 1797-1803, 2004 https://doi.org/10.1248/bpb.27.1797
  17. Fuliang, H. U., Hepburn, H. R., Xuan, H., Chen, M., Daya, S. and Radloff, S. E. : Effects of propolis on blood glucose, blood lipid and free radicals in rats with diabets mellitus Phamacological Research, 51, 147-152, 2005
  18. Wang, N. Z. : Influence of propolis formulations on the blood sugar values of experimental diabetic rabbits Chinese. J. of Veterinary Medicine, 40(4), 7-9, 2004
  19. 한혜경 : 닭의장풀(Commelina communis L.)이 Streptozotocin 유발 당뇨 흰쥐의 혈당에 미치는 영향. 덕성여자대학교 대학원 박사논문, 1995
  20. 고진희 : 고본, 누룩치, 모시대 및 산초의 섭취가 당뇨유발 흰쥐의 혈당수준과 지질대사에 미치는 영향. 덕성여자대학교 대학원 석사학위논문, 2001
  21. 新井成之, 守安純子, 花谷利春, 河井佐知子, 元田龍一, 安藤駿作, 栗本雅司 : プロポリス散劑のラット急性胃潰瘍に對する防禦作用. 醫學の生械學, 124(2), 67-70, 1992
  22. Ramadasan Kuttan : Antidiabetc activity of a herbal preparation. http://business.vsnl.com/cybele/body_test_reports.html
  23. Stanley S. Paphael : Lynch's Medical laboratory technology. 3th, W B Saunder Company, 1031, 1976
  24. Junod AE, Lambert L, Orci R, Pictet, Gonet AE, and Renold AE : Studies of the diabetogenic action of streptozocin. Proc. Soc. Exp. Bioi. Med., 126, 201-205, 1967
  25. John W. Cardinal, Ggeoffrey P. Margison, Kurt. Mynett, Allen P. Yates, Donald P. Cameron, Rhoderick H. Elder : Increased susceptibility to streptozoto-cin-induced $\beta$-cell apoptosis and delayed autoimmune diabetes in alkylpurine-DNA-N-glycosylase-deficient mice. Molecular and Cellular Biology, 21(16), 5605-5613, 2001 https://doi.org/10.1128/MCB.21.16.5605-5613.2001
  26. 이현자, 김석환, 손기호, 최종원 : Equisetum arvense L이 streptozotocin 유발 당뇨성 쥐의 항 당뇨작용에 미치는 영향. 환경연보, 3(1), 45-52, 1993
  27. 박수현, 이연경, 이혜성 : 식이섬유 첨가식이 streptozotocin 유도 당뇨쥐의 장기능과 지질 및 당질대사에 미치는 영향. 한국영양학회지, 27(4), 311-322, 1994
  28. Popper, D. A., Shiau, Y. F. and Reed, M. : Role of small intestine in pathogenesis of hyperlipidemia in diabetic rats. Am. J. Pysiol., 249(12), 161-167, 1985
  29. Lin, S. C, Lin, Y. H., Chen, C. F., Chung, C. Y. and Hsu, S. H. : The hepatoprotective and therapeutic effects of propolis ethanol extract on chronic alcoholinduced liver injuries. American Journal of Chinese Medicine, 25(3-4), 325-332, 1997 https://doi.org/10.1142/S0192415X97000366
  30. Matsushige, K., Basnet, P., Hase, K., Kadota, S., Tanaka, K. and Namba, T. : Propolis protects pancreatic $\beta$-cell against the toxicity of streptozotocin (STZ). Phytomedicine, III (2), 203-209, 1996
  31. 박형기 : 천연항생물질 propolis의 특성과 효용에 대한 고찰. 한국양봉학회지, 9(2), 168-177, 1996
  32. Scheller, S., Stojko, A., Szwarnowiecka, I., Tustanowski, J. and Obuszko, Z. : Influence of EEP on cartilaginous tissue regeneration. Arzneimittel-Forschung (drug reserch), 27, 2138-2140, 1977