한국환경보건학회지 (Journal of Environmental Health Sciences)

  • 제31권1호
  • /
  • Pages.31-38
  • /
  • 2005
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  • 1738-4087(pISSN)
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  • 2233-8616(eISSN)
한국환경보건학회 (Korean Society of Environmental Health)
권호 표지

생물농약개발을 위한 활성미생물의 분리동정에 관한 연구

Isolation and Identification of Activated Microorganisms for Biocide Development

  • 이장훈 (호서대학교 환경안전공학부 환경공학과) ;
  • 강병곤 (호서대학교 환경안전공학부 환경공학과) ;
  • 권혁구 (호서대학교 환경안전공학부 환경공학과) ;
  • 정준오 (호서대학교 환경안전공학부 환경공학과) ;
  • 남윤구 (호서대학교 환경안전공학부 환경공학과)
  • Lee, Jang-Hoon (College of Environment & Safety Engineering, Hoseo University) ;
  • Kang, Byeong-Kon (College of Environment & Safety Engineering, Hoseo University) ;
  • Kwon, Hyuk-Ku (College of Environment & Safety Engineering, Hoseo University) ;
  • Jung, Joon-Oh (College of Environment & Safety Engineering, Hoseo University) ;
  • Nam, Youn-Ku (College of Environment & Safety Engineering, Hoseo University)
  • 발행 : 2005.03.01
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초록

An anti-fungal material produced by actinomycetes was isolated from domestic soil. This actinomycetes was identified as Streptomyces albogriseus by 16S rDNA sequence. YEME (yeast extract 4 g, malt extract 10 g, glucose 4 g, D.W 1l, pH 7.00.2) medium was used for production of anti-fungal materials. S. albogriseus was cultured in a shaking incubator for 2 weeks at 150 rpm and $25^{\circ}C$. An anti-fungal material produced by S. albogriseus was identified at 340 nm by uv/vis- spectrometer and it showed powerful anti-fungal activity. This is the first report that secondary metabolite produced by S. albogriseus showed an activity against phytopathogenic fungi such as Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.

키워드

참고문헌

  1. 이규승 : 농업 생태계에 대한 잔류농약의 영향평가. 한국환경농학회지 16(1), 80-93, 1997
  2. 오윤근, 김정호 : 유기염소계 잔류농약이 제주도 연안 해양환경에 미치는 영향. 한국수질보전학회지, 13(3), 317-324, 1997
  3. 김승현, 이영득, 하원숙, 노희명 : 비포화 무기성 다공매질에서 잔류농약의 계면거동 및 지하수 오염현상 연구. 대한환경공학회지. 20(11), 1545-1553, 1998
  4. Shim, TH, Lee, TJ, Kim, KC, Ryu, MJ, Jung. EH and Lee, HK. : Survey on the ocntents of resodual pesticide in the agricultural products on Kangweon-Do. Kor. J. Food Hygiene, 7(4), 149-156, 1992
  5. Lee, MG and Lee, SR. : Assessment of oncogenicity from pesticide residues in Korean food. Kor. J. Food Sci. Technol., 27(6), 871-877, 1995
  6. Tae Jeon Kim: Lignin을 分解하는 Streptomyces strains에 의한 페놀화합물의 分解. 한국위생학회지, 26(3), 86-91, 2000
  7. Siegel, M and Sisler, HD. : Antifungal compounds, Interactions in Ecological System. Marcel Dekker, Inc., New York, 2, 277, 1977
  8. 최종규, 손현석, 조경태 : B-시클로덱스트린(B-Cyelodextrin)의 결합 특성과 벤젠의 생물학적 분해에의 적용에 대한 연구. 한국환경위생학회지, 28(5), 65-70, 2002
  9. 김창진, 이강현, 아끼라 시마즈, 권오성, 박동진 : 토양특성에 따른 다양한 희소방선균의 분리. 산업미생물학회지, 23(1), 36-42, 1995
  10. Suak Hwan Park, Moon Shik Zong : 抗生物質에 對한 Actimobacillus actinomycetem-comitans의 感受性 및 糖類添加가 細菌의 成長에 미치는 效果, 한국환경위생학회지, 16(2), 127-133, 1990
  11. Lee, D. S., Kim, H. K. and Park, M. Y. : Combination of colony fomation and congo red reaction for detecting intra- and extra- cellular cellulolytic activities. Kor. J. Appl. Microbiol. Bioeng., 12, 305-309, 1984
  12. Lee, E. T. : Antifungal mechanisms and genetic development of antagonistic bacterium on the phytopathgenic fungi. Ph. D. Thesis. Yeungnam University. 1999
  13. Pitcher, D. G., Saunders, N. A. and Owen, R. J. : Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol., 8, 151-156, 1989 https://doi.org/10.1111/j.1472-765X.1989.tb00262.x
  14. Lechevalier, H. A. and Lechevalier, M. P. : The chemotaxonomy of actinomycetes, pp.227-291. In A. Dietz and D. W. Thayer. (ed.), Actinomycete taxonomy, special publication 6. Socoety for Industrial microbiology, Arlington. 1980
  15. Bae, J. Y., Kwon, H. J. and Suh, J. W : Isolation structural determination of antifungal antibiotic from Streptomyces hygroscopicus MJM1004. J. Kor. Soc. Agric. Chem. Biotechnol., 42, 271-276, 1999
  16. Lim, S. D., Yoon, S. K., Lee, M. S., Yoon, W. H. and Kim, C. H. : Isollation and identification of Streptoverticillium sp. NA-4803 producing antifungal substance. Kor. J. Appl. Microbiol. Biotechnol., 24, 664-670, 1996
  17. Suh, J. W, Lim, Y. H., Kim, S. H., Hyun, B. C., Kim, C. O., Yon, C. S., Lee, D. K., Kim, K. P, Jung, J. K. and Lee, C. H. : Biological properties and structural analysis of novel antifungal antibiotics Af-011A. Kor. J. Appl. Microbiol. Biotechnol., 21, 564-569, 1993
  18. Kim, H. R. : Antifungal antibiotics of antagonistic bacterium Bacillus sp. YH-16 against Fusalium solani causing plant root rot. MS. Thesis. Yeungnam University 1994
  19. Lee, N. W., Kim, C. S., Do, J. H., Jung, I. C., Lee, H. W. and Lee, D. H. : Isolation and identification of Bacillus sp. LAM 97-44 producing antifungal antibiotics. J. Kor. Soc. Agric. Chem. Biotechnol., 41, 208-212, 1998.
  20. Kim, C. J., Lee, I. K., Yun, B. S. and Yoo, I. D. : Concanamycin B, active substance against. Phytophythora capsici produced by Streptomyces neyagawaensis 38D10 Strain. Kor. J. Appl. Microbiol. Biotechnol., 21, 322-328, 1993
  21. Kim, S. U., Lee, S. Y., Kim, S. K., Son, K. H., Kim, Y. K., Moon, S. S. and Bok, S. H. : Isolation and characterization of antifungal compound produced by Aspergillus candidus F1484. Kor. J. Appl. Microbiol. Biotechnol., 24, 574-578, 1996
  22. Hong, S. H., Kim, M. J., Park, Y. B., Lee, J. K. and Ha, J. H. : Identification of the antibiotic producing Clostridium sp. KH-43l and purification of the antibiotic. Kor. J. Appl. Microbiol. Biotecnol., 21, 41-46, 1993
  23. Yeo, W. H., Yun, B. S., Whang, K. S., Lee, C. O. and Yu, S. H. : An isochromanequinone compound produced by Micromonospora sp. SA-264. Kor. J. Appl. Microbiol. Biotechnol., 24, 321-326, 1996
  24. Kim, S. S., Joo, G. J., Uhm, J. Y., Kim, Y. J. and Rhee, I. K. : Antifungal activity of Bacillus sp. SS279 and biocontrol of apple white rot fungus, Botryosphaeria dothidea. Kor. J. Appl. Microbiol. Biotechnol., 25, 527-536, 1997
  25. Kim, K. Y. and Kim, S. D. : Biological control of Pyricularia aryzae blast spot with the antibiotic substances produced by Bacillus sp. KL-3. Kor. J. Appl. Microbiol. Biotechnol., 25, 396-402, 1997
  26. Lim, H. S. : Genetic development of siderophore-producing Pseudomonas fluorescens GL20 as a biological control agent. Ph. D. Thesis. Yeungnarn University. 1997