Detection of Staphylococcus aureus and Screening Staphylococcal Enterotoxin a, b, c genes in Strains Isolated from Strawberry Juice Shops in Jinju

진주 지역 딸기 주스 상점에서의 Staphylococcus aureus의 분리와 staphylococcal enterotoxin a, b, c gene 검색

  • Kim, Se-Ri (Division of Appled Life Science, Gyeongsang National University) ;
  • Park, Seon-Ja (Department of Anatomy, College of Medicine, Gyeongsang National University) ;
  • Shim, Won-Bo (Division of Appled Life Science, Gyeongsang National University) ;
  • Kim, Hyoung-Kab (Department of Environmental Engineering, Jinju National University) ;
  • Chung, Duck-Hwa (Division of Appled Life Science, Gyeongsang National University)
  • 김세리 (경상대학교 응용생명과학부) ;
  • 박선자 (경상대학교 의과대학) ;
  • 심원보 (경상대학교 응용생명과학부) ;
  • 김형갑 (진주산업대학교 환경공학과) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Published : 2005.03.01

Abstract

Staphylococcus aureus is one of the important pathogenic agents, which are related to the hygienic condition. This study performed for the detection of Staphylococcus aureus and screening staphylococcal enterotoxin a, b, c genes in strains isolated from the environment for production of non-pasteurized strawberry juice. A total of 44 samples were collected from utensils, machinery, employees, raw materials, and strawberry juices in 3 strawberry juice shops in Jinju, western Gyeongnam. The isolation rate of Staphylococcus aureus was 26%. Specially Staphylococcus aureus was frequently isolated from employee's hands, strawberry and strawberry juices. The sea, seb, and sec genes were also investigated by polymerase chain reaction (PCR). One hundred and 55% of each isolate had found sea gene and seb gene, respectively. However, sec gene was not detected anywhere. To prevent food-borne disease associated with juice, the accomplishment of HACCP to be more efficient and systematic is necessary.

Keywords

References

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