Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Study on Medium Ingredient Composition for Enhancing Biomass Productionand Anti-potato Common Scab Activity of Streptomyces sp. A020645 as a BCA Candidate

생물제제(BCA) 후보균주인 Streptomyces sp. A020645 의 대량 균체생산 및 항더뎅이병 활성증진을 위한 고체배지 조성에 관한 연구

  • Lee, Hyang-Burm (School of Biological Sciences, Seoul National University) ;
  • Roh, Hyo-Young (Div. of Drug Discovery, Korea Research Institute of Bioscience & Biotechnology) ;
  • Park, Dong-Jin (Div. of Drug Discovery, Korea Research Institute of Bioscience & Biotechnology) ;
  • Lee, So-Keum (Div. of Drug Discovery, Korea Research Institute of Bioscience & Biotechnology,Dept. of Food Science and Engineering, Cheju National University) ;
  • Ko, Young-wan (Dept. of Food Science and Engineering, Cheju National University) ;
  • Koh, Jeong-Sam (College of Agricultural and Life Science, Cheju National University) ;
  • Kim, Chang-Jin (Div. of Drug Discovery, Korea Research Institute of Bioscience & Biotechnology)
  • 이향범 (서울대학교 생명과학부) ;
  • 노효영 (한국생명공학연구원 바이오신약연구부) ;
  • 박동진 (한국생명공학연구원 바이오신약연구부) ;
  • 이소금 (한국생명공학연구원 바이오신약연구부, 제주대학교 식품공학과) ;
  • 고영환 (제주대학교 식품공학과) ;
  • 고정삼 (제주대학교 농업생명과학대학) ;
  • 김창진 (한국생명공학연구원 바이오신약연구부)
  • Published : 2005.06.01
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Abstract

The effect of medium components such as wheat bran, rice bran, oat meal, and soybean meal as basic ingredients and KH2PO4, glucose, and molasses as additives on mass production and anti-potato common scab activ ity of a streptomycete A020645 strain as a biocontrol agent (BCA) candidate was investigated. Of basicingredients, oat meal was the best one for mass poduction and enhancement of anti-potato common scabactivity. The biomass production of the active strain was more enhanced when 0.1-0.01.% glucose or molassesas additive were added into the basic medium. These information may have important implications in applying for effective formulation of BCA.

본 연구는 감자 더뎅이병원균에 대한 항균활성을 갖는 Streptomyces sp. A020645 균주를 대상으로 최적의 고체 배양 및 항더뎅이병 활성증진 배지조성 조건을 검토하고자 실시하였다. 본 실험에 사용한 배지의 조성을 보면 밀기울, 쌀겨, 오트밀, 대두박을 기본 성분으로, 그리고 $KH_2PO_4$, 포도당, 당밀을 첨가물로 사용하였다. 연구결과 포자생성은 오트밀배지를 주성분으로 하고 glucose 0.1~0.01%, 당밀 1.1~0.01% 첨가배지에서 포자생성이 매우 양호하였다 그 외에도 $KH_2PO_4$ 2%를 첨가한 경우와 포도당과 당밀의 농도를 달리하였을 경우도 양호한 균체생성을 확인하였다. Streptomyces sp. A020645 균주의 배지소성별 항더뎅이병원균에 대한 활성을 조사한 결과 오트밀 및 쌀겨 배지를 주성분으로 사용할 경우 glucose 0.1~0.01%, 당밀 0.1% 첨가 조건에서 가장 높은 항균활성을 나타냈다. 이러한 연구결과는 생물제제 제형개발을 위한 기초자료로서 유용하게 활용될 수 있을 것이다.

Keywords

References

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