The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Identification, Growth and Pathogenicity of Colletotrichum boninense Causing Leaf Anthracnose on Japanese Spindle Tree

  • Lee, Hyang-Burm (Department of Biological Sciences, Seoul National University) ;
  • Park, Jae-Young (Department of Biological Sciences, Seoul National University) ;
  • Jung, Hack-Sung (Department of Biological Sciences, Seoul National University)
  • Published : 2005.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Leaf anthracnose was observed on leaves of Japanese spindle tree in Seoul, Korea from autumn 2003 to spring 2004. The causal fungus was purely isolated from he leaf spot lesions and cultured on PDA. The colony on PDA was cream to orange but blackish in the center n old cultures. Conidia were formed in blackish orange asses and were cylindrical in shape, measured 13-17${\times}$5-7 ${\mu}$m in size. Blackish brown setae were often observed on PDA and ranged up to 100 ${\mu}$m in length. Based on morphological and ITS region sequence analyses, the fungal strain was identified as Colletotrichum boninense. Koch’s postulates were fulfilled by inoculating tree leaves with 1 ${\times}$ $106^6$ conidia per ml in a moist chamber. This is the first study on the pathogenicity, growth and phylogenetic characteristics of C. boninense causing leaf anthracnose on Japanese spindle tree in Korea.

Keywords

References

  1. Alfieri Jr, S. A., Langdon, K. R., Wehlburg, C. and Kimbrough, J. W. 1984. Index of Plant Disease in Florida (Revised). Florida Dept. Agric. and Consumer Serv., Div. Plant Ind. Bull. 11: 389
  2. Barnett, H. L. and Hunter, B. B. 1987. Illustrated genera of imperfect fungi. 4th ed. Macmillan Publishing Company. NewYork, USA
  3. Chun J. 1995. Computer-assisted classification and identification of actinomycetes. PhD thesis, Univ. of New Castle
  4. Crous, P. W., Denman, S., Taylor, J. E., Swart, L. and Palm, M. E. 2004. Cultivation and diseases of Proteaceae: Leucadendron, Leucospermum and Protea. Centraalbureau voor Schimmelcultures, Utrecht. 227 p
  5. Grand, L. F. 1985. North Carolina Plant Disease Index. North Carolina Agric. Res. Serv. Techn. Bull. 240:1-157
  6. Hilton, S. 2000. Canadian Plant Disease Survey. Agriculture and Agri-Food Canada 80:151
  7. Holliday, P. 1980. Fungus disease of tropical crops. Cambridge University Press, Cambridge, UK
  8. International Seed Testing Association (ISTA). 1976. Seed Sci. Technol. 4:3-49
  9. Korea Forest Research Institute. 1991. Insect Pest and Disease of Trees and Shrubs. p. 280. Korea Forest Service
  10. Lee, H. B., Park, J. Y. and Jung, H. S. 2005. First report of leaf anthracnose caused by Colletotrichum boninense on spindle trees. Plant Pathol. 54, in press
  11. Lu, G., Cannon, P. F., Reid, A. and Simmons, C. M. 2004. Diversity and molecular relationships of endophytic Colletotrichum isolates from the Iwokrama Forest Reserve, Guyana. Mycol. Res. 108:53-63 https://doi.org/10.1017/S0953756203008906
  12. Mahoney, M. J. and Tattar, T. A. 1980. Identification, etiology, and control of Euonymus fortunei anthracnose caused by Colletotrichum gloeosporioides. Plant Dis. 64:854-856 https://doi.org/10.1094/PD-64-854
  13. Moriwaki, J., Sato, T. and Tsukiboshi, T. 2003. Morphological and molecular characterization of Colletotrichum boninense sp. nov. from Japan. Mycoscience 44:47-53 https://doi.org/10.1007/s10267-002-0079-7
  14. Shivas, R. G., Bathgate, J. and Podger, F. D. 1998. Colletotrichum xanthorrhoeae sp. nov. on Xanthorrhoea in Western Australia. Mycol. Res. 102:280-282 https://doi.org/10.1017/S0953756297004760
  15. Sutton, B. C. 1980. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew, UK, pp. 523-527
  16. Sutton, B. C. 1992. The genus Glomerella and its anamorph Colletotrichum. In: Colletotrichum: biology, pathology and control, ed. by J. A., Bailey and M. J. Jeger, pp. 1-26. CAB International, Wallingford, UK
  17. Swofford, D. L. 2002. PAUP: Phylogenetic analysis using parsimony, Version 4.0b10. Illinois Natural History Survey, Champaign, IL
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgin, D. G. 1997. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 24:4876-4882
  19. USDA, 1960. Index of plant diseases in the United States. Agric. Handb. No. 165. US Gov. Print. Off., Washington, DC. 531 pp
  20. van der Aa, H. 1978. A leaf spot disease of Nymphaea albu in the Netherlands. Neth. J. Plant Pathol. 84:109-115 https://doi.org/10.1007/BF01981538
  21. von Arx, J. A. 1957. Die Arten der Gattung Colletotrichum Corda. Phytopathol. Z. 29:413-468
  22. von Arx, J. A. 1981. The genera of fungi sporulating in pure culture. 3rd ed. Cramer, Vaduz, Germany, pp. 220-223
  23. Waller, J. M., Bridge, B. D., Black, R. and Hakiza, G. 1993. Characterization of the coffee berry disease pathogen, Colletotrichum kahawae sp. nov. Mycol. Res. 97:989-994 https://doi.org/10.1016/S0953-7562(09)80867-8
  24. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, ed. by M. A. Innis, D. H. Gelfand and J. J. Sninsky, pp. 315-322. Academic Press, London, UK

Cited by

  1. Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent vol.24, pp.4, 2008, https://doi.org/10.5423/PPJ.2008.24.4.453
  2. The Colletotrichum boninense species complex vol.73, 2012, https://doi.org/10.3114/sim0002
  3. Colletotrichum – current status and future directions vol.73, 2012, https://doi.org/10.3114/sim0014
  4. Geographic locality greatly influences fungal endophyte communities in Cephalotaxus harringtonia vol.117, pp.2, 2013, https://doi.org/10.1016/j.funbio.2012.12.005