Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of Diclofenac, Acetamonophen, Nimesulide and Acetylsalicylic Acid on Mucin Release from Cultured Hamster Tracheal Surface Epthelial Cells

  • HEO Ho Jin (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • LEE Hyun Jae (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • YOON Chi Soon (Department of Thoracic Surgery, Chungnam National University Hospital) ;
  • LIM Seung Pyong (Department of Thoracic Surgery, Chungnam National University Hospital) ;
  • SEOK Jeong Ho (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • LEE Choong Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
  • Published : 2005.12.01
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Abstract

In this study, we tried to investigate whether diclofenac, acetaminophen, nimesulide, acetylsalicylic acid and tumor necrosis factor-alpha (TNF-alpha) significantly affect mucin release from cultured airway goblet cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with $^3H$-glucosamine for 24 hr and chased for 30 min or 24 hr in the presence of each agent to assess the effects on $^3H$-mucin release. The results were as follows: (1) TNF-alpha significantly increased mucin release from cultured HTSE cells during 24 hr of treatment period; (2) However, diclofenac, acetaminophen, nimesulide and acetylsalicylic acid did not affect mucin release, during 30 min of treatment period. Basically, this finding suggests that non-steroidal antiinflammatory drugs (NSAIDs) might not function as a mucoregulator in various inflammatory respiratory diseases showing mucus hypersecretion, although further studies are needed.

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References

  1. Chung, K.F. (2001). Cytokines in chronic obstructive pulmonary disease. Eur. Respir. J. Suppl. 34, 50-59
  2. Cohn, L., Whittaker, L., Niu, N., Homer, R.J. (2002). Cytokine regulation of mucus production in a model of allergic asthma. Novartis. Found. Symp. 248,201-213
  3. Ellis, E.F. (1985). Asthma in childhood. J. Allergy Clin. Immunol. 72 (supp!), 526-539
  4. Fischer, B.M., Rochelle, L.G, Voynow, J.A., Akley, N.J., Adler, K.B. (1999). TNF-alpha stimulates mucin secretion and cyclic GMP production by guinea pig tracheal epithelial cells in vitro. Am. J. Respir. Cell. Mol. BioI. 20,413-422 https://doi.org/10.1165/ajrcmb.20.3.3393
  5. Gray, T., Nettesheim, P., Basbaum, C., and Koo, J. (2001). Regulation of mucin gene expression in human tracheobronchial epithelial cells by thyroid hormone. Biochem. J. 353, 727-734 https://doi.org/10.1042/0264-6021:3530727
  6. Hauber, H.P., Daigneault, P., Frenkiel, S., Lavigne, F, Hung, H.L., Levitt, R.C., Hamid, Q. (2005). Niflumic acid and MSI-2216 reduce TNF-alpha-induced mucin expression in human airway mucosa. J. Allergy Clin. Immunol. 115(2),266-271 https://doi.org/10.1016/j.jaci.2004.09.039
  7. Jeffery, P.K (1986). Anti-inflammatory drugs and experimental bronchitis. Eur. J. Respir. Dis. 146,245-257
  8. Kim, Y., Kwon, E., Park, D., Song, S., Yoon, S., Baek, S. (2002) IL-l beta induces MUC2 and MUC5AC synthesis through cyclooxygenase-2 in NCI-H292 cells. Mol. Pharmacol. 62, 1112-1118 https://doi.org/10.1124/mol.62.5.1112
  9. Kim, K.C., McCracken, K, Lee, B.C., Shin, C.Y., Jo, M.-J, Lee, C.J. and Ko, K.H. (1997). Airway goblet cell mucin: its structure and regulation of secretion. Eur. Respir. J. 11, 2644-2649
  10. Kim, KC., Rearick, J.I., Nettesheim, P., and Jetten, A.M. (1985). Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J. Biol. Chem. 260,4021-4027
  11. Kim, K.C., Wasano, K, Niles, R.M., Schuster, J.E., Stone, P.J. and Brody, J.S. (1987). Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proc. Natl. Acad. Sci. USA 84, 9304-9308
  12. Ko, KH., Lee, C.J., Shin, C.Y, Jo, M.-J. and Kim, K.C. (1999). Inhibition of mucin release from airway goblet cells by polycationic peptides. Am. J. Physiol. 277(21), L811-L815
  13. Lee, C.J., Paik, S.H., Ko, K.H., Kim, K.C. (2002). Effects of polycationic peptides on mucin release from airway goblet cells: relationship between polymer size and activity. Inflamm. Res. 51(10),490-494 https://doi.org/10.1007/PL00012417
  14. Mutschler, E., Derendorf, H. (1995). Drug Actions, pp. 410-411, CRC press, Boca Raton, Florida
  15. Roger, P., Gascard, J.P., Bara, J., De Montpreville, V.T., Brink, C. (2001). MUC5AC mucin release from human airways in vitro: effects of indomethacin and Bay X1005. Mediators. Inflamm. 10(1), 33-36 https://doi.org/10.1080/09629350124329
  16. Shao, M.X., Ueki, I.F, Nadel, J.A (2003). TNF-alpha converting enzyme mediated MUC5AC mucin expression in cultured human airway epithelial cells. Proc. Natl. Acad. Sci. USA 100(20), 11618-11623
  17. Song, K.S., Lee, W.J, Chung, K.C., Koo, J.S., Yang, E.J., Choi, J.Y., Yoon, J.H. (2003). IL-lbeta and TNF-alpha induced MUC5AC overexpression through a mechanism involving ERK/p38 mitogen-activated protein kinase-MSKI-CREB activation in human airway epithelial cells. J. BioI. Chem. 278(26), 23243-23250 https://doi.org/10.1074/jbc.M300096200
  18. Takeyama, K, Dabbagh, K, Lee, H., Agusti, C; Lausier, J.A, Ueki, I.F, Grattan, K.M. and Nadel, J.A (1999). Epidermal growth factor system regulates mucin production in airways. Proc. Natl. Acad. Sci. USA 6, 3081-3086
  19. Wasano, K, Kim, K.C., Niles, R.M., and Brody, J.S. (1988) Membrane differentiation markers of airway epithelial secretory cells. J. Histochem. Cytochem. 36, 167-178 https://doi.org/10.1177/36.2.3335774