Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Chunghyuldan Downregulates the Activation of Transcription Factors NF - kB and AP-1 of BV-2 Cells Induced by Lipopolysaccharide

  • WEE Sung-SooK (College of Pharmacy, Kyung Hee University) ;
  • BAE Eun-Ah (College of Pharmacy, Kyung Hee University) ;
  • PARK Jin-Sun (Department of Neuroscience, Ewha Womans University Medical School) ;
  • KIM Hee-Sun (Department of Neuroscience, Ewha Womans University Medical School) ;
  • CHo Hee Jae (Kwangdong Central Research Institute, Kwangdong Pharm. Co., Ltd,) ;
  • Ryu Jong-Hoon (College of Pharmacy, Kyung Hee University) ;
  • KIM Dong-Hyun (College of Pharmacy, Kyung Hee University)
  • Published : 2005.12.01
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Abstract

Chunghyuldan (Qingxuedan in Chinese) (CHD) has been used for patients with atherosclerosis and brain ischemia in Korea. To evaluate antiischemic activity of CHD, its antiinflammatory effect in lipopolysaccharide-induced BV-2 cells was investigated. CHD potently inhibited nitric oxide (NO) production in LPS-induced BV-2 cells with an $IC_{50}$ value of 4.8${\mu}g/ml$. CHD did not only inhibit mRNA and protein expression levels of inducible NO synthase and cyclooxygenase-2 in LPS-induced BV-2 cells, but also repressed mRNA expression levels of proinflammatory cytokines IL-l$\beta$ and TNF-$\alpha$. CHD also downregulated the activation of NF-kB and AP-l transcription factors induced by LPS. These results suggest that CHD may improve inflammatory brain ischemia by the downregulation the activation of NF-kB and AP-l transcription factors.

Keywords

References

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