Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparative Analyses of Flavonoids for nod Gene Induction in Bradyrhizobium japonicum USDA110

  • RYU JI-YOUNG (International Environmental Research Center, UNU-GIST Joint Program on Science and Technology for Sustainability, and Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology) ;
  • HUR HOR-GIL (International Environmental Research Center, UNU-GIST Joint Program on Science and Technology for Sustainability, and Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology)
  • Published : 2005.12.01
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Abstract

Using the nodY::lacZ fusion system in Bradyrhizobium japonicum USDA 110, 22 flavonoids, which have structurally different features, were tested to define the role of the substituted functional groups as an inducer or inhibitor for the nod gene expression. A functional ,group of 4'-OH on the B-ring and the double bond between 2-C and 3-C on the C ring were required to induce the nod gene expression in B. japonicum USDA 110. In the case of isoflavones, the 4'-methoxyl group, which blocks the open 4'-OH functional group, did not significantly lower inducing activity, as compared with isoflavones with 4'-OH. However, all flavonols tested, which have a 3-OH functional group on the C-ring, did not induce, but inhibited the nod gene expression. Flavone, 7-hydroxyflavone, and kaempferol (5,7,4'-trihydroxyflavonol) at $1\;{\mu}M$ concentration significantly inhibited the nod gene expression induced by 7,4'-dihydroxyflavone. However, 7-hydroxy-4'-methoxyflavone at $1\;{\mu}M$ concentration showed a synergistic effect with genistein and 7,4'-dihydroxyflavone on the induction activity.

Keywords

References

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