Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzymatic Methanolysis of Castor Oil for the Synthesis of Methyl Ricinoleate in a Solvent-Free Medium

  • YANG JUNG-SEOK (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • JEON GYU-JONG (Samgsung Fine Chemical Co. Ltd.) ;
  • HUR BYUNG-KI (School of Chemical, Polymer, and Biological Engineering, lnha University) ;
  • YANG JI-WON (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 2005.12.01
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Abstract

Several lipases of commercial grade were screened to catalyze the methanolysis of castor oil, and an immobilized Candida antarctica (Novozym 435) had the highest activity among the lipases tested. To enhance the yield of methyl ricinoleate, several reaction parameters were optimized. The optimum temperature was $50^{\circ}C$, and the original water content of lipase was sufficient to maintain the activity of lipase, and additional water supplied inhibited the methanolysis of castor oil. Because the lipase was deactivated by methanol, the reaction was tested by three-step addition of 1 molar equivalent of methanol to the oil. However, the oil was not completely converted to its methyl esters. The final reaction mixture using 3 molar equivalents of methanol to the oil consisted of $70\%$ methyl ricinoleate, $18\%$ monoricinoleate, $11\%$ diricinoleate, and trace triricinoleate at the equilibrium state. The yield of methyl ricinoleate was $97\%$ at 6 molar ratio of methanol to the oil with 300g of castor oil and 6g of immobilized Candida antarctica at $50^{\circ}C$ within 24 h.

Keywords

References

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