Prediction on the Stability of Spray-Dried Lactobacillus reuteri KUB-AC5 by Arrhenius Equation for Long-Term Storage

  • KORAKOCH HAMSUPO (Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University) ;
  • SUKYAI PRAKIT (Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University) ;
  • LOISEAU GERARD (Centre de cooperation Internationale en Recherche Agronomique pour le Developpement, Departement AMIS, UR Tropiqual) ;
  • NITISINPRASERT SUNEE (Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University) ;
  • MONTET DIDIER (Centre de cooperation Internationale en Recherche Agronomique pour le Developpement, Departement AMIS, UR Tropiqual) ;
  • WANCHAITANAWONG PENKHAE (Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University)
  • Published : 2005.12.01


Survival of thermotolerant Lactobacillus reuteri KUB-AC5 in $20\%$ (w/v) skim milk was found to be $11.3\%$ after spray drying by using a pilot scale spray dryer with inlet temperature at $170^{\circ}C$ and outlet temperature at $85^{\circ}C$. The ability of dried cell to produce antimicrobial activity was not affected by the spray drying. The model system for predicting viability of spray-dried L. reuteri KUB-AC5 during long-term storage was established, based on the Arrhenius equation, and verified by experimental data, because the viability of cells during storage can be correlated with storage temperature. The viability during storage at $30^{\circ}C$ declined more rapidly than that storage at $4^{\circ}C$.



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