한국축산식품학회지 (Food Science of Animal Resources)

  • 제25권4호
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  • Pages.494-499
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  • 2005
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
권호 표지

유아의 분변에서 분리한 Lactobacillus gasseri KACC 91155의 Jurkat T Cells에서 항산화 효과

The Antioxidant Effect of Lactobacillus gasseri KACC 91155 Isolated from Korean Infant in Jurkat T Cells

  • 발행 : 2005.12.01
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초록

본 연구에서는 유아의 분변에서 분리한 항산화 효과를 갖는 유산균인 Lactobacillus gasseri KACC 91155의 Jurkat T cell line에서의 지질 과산화 억제 효과, 세포 보호 효과 및 DNA 손상 억제 효과를 측정하였다. 그 결과 1. gasseri 91155는 세포 산화에 의해 생성된 지질 과산화물인 MDA의 생성을 방지하였으며, 또한 뚜렷하게 세포 생존성을 증가시켰다. 그리고 산화적 손상으로부터 DNA 손상을 줄여주는 효과를 나타내었다.

In the present study, we investigate the protective effect of antioxidant strain Lactobacillus gasseri KACC 91155, isolated from Korean infant feces(Obstetrics & Gynecology, Suwon, Korea) on the oxidative stress damage on the Jurkat T cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA(malondialdehyde) production was measured Furthermore, cell viability was detected by the MTT assay, DNA damage was tested by the comet assay. Cell grown in medium with or without L gasseri lysate$(100\~1,000{\mu}g)$ were treated with $H_2O_2,\;Fe^{2+}$ as an oxidative stimulus. From the result obtained, the supplementation of Jurkat T cells with L. gasseri lysate significantly decreased in MDA production (1,250 vs. 835 nmol/mg protein), and DNA damage(31.6 vs. 22.6 tail moment). Also L gasseri increase cell viability against oxidative damage. We concluded that the L. gasseri KACC 91155 showed a protective effect against oxidative stress.

키워드

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