한국어병학회지 (Journal of fish pathology)

  • 제18권2호
  • /
  • Pages.179-185
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  • 2005
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

과망간산칼륨, 안정화이산화염소, 포르말린, 황산동이 넙치(Paralichthys olivaceus) 적혈구에 미치는 시험관내 용혈작용 및 메트헤모글로빈 생성 효과

In Vitro Hemolysis and Methemoglobin Formation in Olive Flounder (Paralichthys olivaceus) Erythrocytes Induced by Potassium Permanganate, Stabilized Chlorine Dioxide, Formalin and Copper Sulphate

  • 정승희 (국립수산과학원 병리연구팀) ;
  • 김진우 (국립수산과학원 병리연구팀)
  • Jung, Sung-Hee (Pathology Team, National Fisheries Research & Development Institute) ;
  • Kim, Jin-Woo (Pathology Team, National Fisheries Research & Development Institute)
  • 발행 : 20050800
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초록

과망간산칼륨 ($KMnO_4$), 안정화이산화염소 ($S-ClO_2$, 3% 수용액), 포르말린 (37% formaldehyde), 황산동 ($CuSO_4$)을 이용하여 넙치 적혈구의 cell damage에 미치는 효과를 알아보고자 in vitro 용혈작용과 methemoglobin (MetHb) 생성을 조사하였다. 실험의 농도는 과망간산칼륨 2-250 ppm, 안정화이산화염소 3.13-400 ppm, 포르말린 31.3-2,000 ppm, 황산동 0.04-5 ppm 이었다. 과망간산칼륨은 31.3-250 ppm, 황산동은 0.63 -5 ppm에서 현저한 용혈작용을 나타내었으나 이와는 반대로 MetHb 생성은 전혀 없었다. 따라서 과망간산칼륨과 황산동은 넙치 적혈구에 대한 cell damage system이 매우 유사한 것으로 생각되었다. 안정화이산화염소의 경우, 용혈작용은 25 ppm 이상의 농도에서 그리고 MetHb 생성은 6.25 ppm 이상의 농도에서 높게 증가하였다. 본 실험에서 유일하게 용혈작용과 MetHb 생성을 양쪽 다 현저하게 나타내었다. 포르말린은 2,000 ppm의 고농도에서도 용혈작용은 없었으며, MetHb 생성은 250-2,000 ppm에서 완만하게 증가하였다. 따라서 안정화이산화염소와 포르말린은 넙치 적혈구에 대하여 과망간산칼륨 및 황산동과는 또 다른 cell damage system을 가지고 있는 것으로 나타났다.

In Vitro hemolysis and methemoglobin (MetHb) formation in olive flounder rythrocytes were investigated using potassium permanganate ($KMnO_4$) ranging from 2 to 250 ppm, stabilized chlorine dioxide ($S-ClO_2$)ranging from 3.13 to 400 ppm, formalin (37% formaldehyde) ranging from 31.3 to 2,000 ppm and copper sulphate ($CuSO_4$) ranging from 0.04 to 5 ppm. Remarkable hemolysis was found to be induced at $KMnO_4$ concentrations of 31.3-250 ppm and $CuSO_4$ concentrations of 0.63-5 ppm. On the other hand, MetHb formation could not be found at the same treatment concentrations. It is suggested that the cell-damaging system of $KMnO_4$ may be similar from that of $CuSO_4$ in the erythrocytes of olive flounder. Remarkable hemolysis and MetHb formation were found to be induced at $S-ClO_4$ concentrations of more than 25 ppm and 6.25 ppm, respectively. Only $S-ClO_2$ showed both hemolysis and MetHb formation among the chemicals used in the present study. Formalin did not provoke hemolysis at the highest concentration of 2,000 ppm but induced MetHb formation at ranging from 250 to 2,000 ppm. These findings reveal that the mechanism involved in formalin-induced cell-damaging effects differs from that induced by $S-ClO_2$ to olive flounder erythrocytes compared with $KMnO_4$ and $CuSO_4$.

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