BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지
DOI QR코드

DOI QR Code

Genetic Heterogeneity of the Tropical Abalone (Haliotis asinina) Revealed by RAPD and Microsatellite Analyses

  • Tang, Sureerat (Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Popongviwat, Aporn (Department of Marine Science, Faculty of Science, Chulalongkorn University) ;
  • Klinbunga, Sirawut (Marine Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)) ;
  • Tassanakajon, Anchalee (Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Jarayabhand, Padermsak (Department of Marine Science, Faculty of Science, Chulalongkorn University) ;
  • Menasveta, Piamsak (Department of Marine Science, Faculty of Science, Chulalongkorn University)
  • 발행 : 2005.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Genetic heterogeneity of the tropical abalone, Haliotis asinina was examined using randomly amplified polymorphic DNA (RAPD) and microsatellite analyses. One hundred and thirteen polymorphic RAPD fragments were generated. The percentage of polymorphic bands of H. asinina across overall primers was 85.20%. The average genetic distance of natural samples within the Gulf of Thailand (HACAME and HASAME) was 0.0219. Larger distance was observed when those samples were compare with HATRAW from the Andaman Sea (0.2309 and 0.2314). Geographic heterogeneity and $F_{ST}$ analyses revealed population differentiation between H. asinina from the Gulf of Thailand and the Andaman Sea (p < 0.0001). Three microsatellite loci (CUHas1, CUHas4 and CUHas5) indicated relatively high genetic diversity in H. asinina (total number of alleles = 26, 5, 23 and observed heterozygosity = 0.84, 0.42 and 0.33, respectively). Significant population differentiation was also found between samples from different coastal regions (p < 0.0001). Therefore, the gene pool of natural H. asinina in coastal Thai waters can be genetically divided to 2 different populations; the Gulf of Thailand (A) and the Andaman Sea (B).

키워드

참고문헌

  1. Amparyup, P., Klinbunga, S., Preechaphol, R., Tassanakajon, A., Hirono, I., Aoki, T. and Jarayabhand, P. (2005) Expressed sequence tag analysis of ovaries and testes from the tropical abalone (Haliotis asinina). Mar. Biotechnol. (in press)
  2. Brown, L. D. (1991) Genetic variation and population structure in the blacklip abalone, Haliotis rubra. Australian J. Mar Freshwater Res. 42, 77-90 https://doi.org/10.1071/MF9910077
  3. Chen, H. C. (1989) Farming the small abalone Haliotis diversicolor supertexta in Taiwan; in Handbook of Culture of Abalone and Other Marine Gastropods, Hahn, K.O. (ed.), pp. 265-283, CRC Press, Boca Raton, USA
  4. Crow, J. F. and Kimura, M. (1965) Evolution and sexual and asexual populations. Am. Nature 99, 439-450 https://doi.org/10.1086/282389
  5. Evans, B., Conod, N. and Elliott, N. G. (2001) Evaluation of microsatellite primer conservation in abalone. J. Shellfish Res. 20, 1065-1070
  6. Fritsch, P., Hanson, M. A., Spore, C. D., Pack, P. E. and Rieseberg, L. H. (1993) Constancy of RAPD primer amplification strength among distantly related taxa of flowering plants. Plant Mol. Biol. Rep. 11, 10-20 https://doi.org/10.1007/BF02670555
  7. Felsenstein, J. (1993) Phylip (Phylogenetic Inference Package) Phylogenetic Inference Package version 3.5c. Department of Genetics, University of Washington, Seattle, USA
  8. Geiger, D. L. (2000) Distribution and biogeography of the Haliotidae Gastropoda: Vetigastropod world-wide. Bollettino Malacologico 35, 57-120
  9. Gordon, H. R. (2000) World abalone supply, markets and pricing: historical, current and future prospective. The 4th International Abalone Symposium, 6-11 February 2000, University of Cape town, Cape Town, South Africa
  10. Guo, S. W. and Thompson, E. A. (1992) Performing the extract test of Hardy-Weinberg proportion for multiple alleles. Biometrics 48, 361-372 https://doi.org/10.2307/2532296
  11. Hadrys, H., Balick, M., and Schierwater, B. (1992) Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol. Ecol. 1, 55-63 https://doi.org/10.1111/j.1365-294X.1992.tb00155.x
  12. Huang, B. and Hanna P. J. (1998) Identification of three polymorphic microsatellite loci in blacklip abalone, Haliotis rubra Leach, and detection in other abalone species. J. Shellfish Res. 17, 795-799
  13. Huang, B. X., Peakall, R. and Hanna, P. J. (2000) Analysis of genetic structure of blackip abalone Haliotis rubra populations using RAPD, minisatellite and microsatellite markers. Mar. Biol. 136, 207-216 https://doi.org/10.1007/s002270050678
  14. Jarayabhand, P. and Paphavasit, N. (1996) A review of the culture of tropical abalone with special reference to Thailand. Aquaculture 140, 159-168 https://doi.org/10.1016/0044-8486(95)01194-3
  15. Jarayabhand, P., Pripue, P., Klinbunga, S. and Tassanakajon, A. (2002) Identification of species-diagnostic markers of abalone in Thailand using PCR-RFLP of 16S rDNA. Fisheries Sci. 68, 1091-1094
  16. Kirby, V. L., Villa, R. and Powers, D. A. (1998) Identification of microsatellites in the California red abalone, Haliotis rufescens. J. Shellfish Res. 17, 801-804
  17. Klinbunga, S., Amparyup, P., Leelatanawit, R., Tassanakajon, A., Hirono, I., Aoki, T., Jarayabhand, A. and Menasveta, P. (2004) Species-identification of the tropical abalone Haliotis asinina, Haliotis ovina and Haliotis varia in Thailand using RAPD and SCAR markers. J. Biochem. Mol. Biol. 37, 213-222 https://doi.org/10.5483/BMBRep.2004.37.2.213
  18. Klinbunga, S., Ampayup, P., Tassanakajon, A., Jarayabhand, P. and Yoosukh, W. (2001) Genetic diversity and molecular markers of commercial oysters Genera Crassostrea, Saccostrea and Striostrea in Thailand determined by RAPD analysis. Mar. Biotechnol. 3, 133-144 https://doi.org/10.1007/s101260000057
  19. Klinbunga, S., Boonyapakdee, A. and Pratoomchat, B. (2000) Genetic diversity and species-diagnostic markers of mud crabs Genus Scylla in Eastern Thailand determined by RAPD analysis. Mar. Biotechnol. 2, 180-187
  20. Klinbunga, S., Pripue, P., Khamnamtong, N., Puanglarp, N., Tassanakajon, A., Jarayabhand, P. and Menasveta, P. (2003) Genetic diversity and molecular markers of the tropical abalone Haliotis asinina in Thailand. Mar. Biotechnol. 5, 505-517 https://doi.org/10.1007/s10126-002-0108-8
  21. Lynch, M. (1990) The similarity index and DNA fingerprinting. Mol. Biol. Evol. 7, 478-484
  22. Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, USA
  23. Metz, E. C., Robles-Sikisaka, R. and Vacquier, V. D. (1998) Nonsynonymous substitution in abalone sperm fertilization genes exceeds substitution in introns and mitochondrial DNA. Proc. Nat. Acad. Sci. USA 95, 10676-10681 https://doi.org/10.1073/pnas.95.18.10676
  24. Miller, K. M., Laberee, K., Kaukinen, K. H., Li, S. and Withler, R. E. (2001) Development of microsatellite loci in pinto abalone Haliotis kamtschatkana. Mol. Ecol. Notes 1, 315-317 https://doi.org/10.1046/j.1471-8278.2001.00122.x
  25. Nateewatana, A. and Bussarawit, S. (1988) Abundance and distribution of abalone along the Andaman Sea coast of Thailand. Kasetsart J. Science 22, 8-15
  26. Nei, M. (1987) Genetic variation within species; in Molecular Evolutionary Genetics, pp. 176-207, Columbia University Press, New York, USA
  27. Nei, M. and Li, W. H. (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA 76, 5269-5273 https://doi.org/10.1073/pnas.76.10.5269
  28. Raymond, M. and Rousset, F. (1995) Genepop (version 1.2): a population genetics solfware for exact tests and ecumenicism. J. Heredity 86, 248-249
  29. Rice, W. R. (1989) Analyzing tables of statistical tests. Evolution 43, 223-225 https://doi.org/10.2307/2409177
  30. Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenic trees. Mol. Biol. Evol. 4, 406-425
  31. Sekino, M. and Hara, M. (2001) Microsatellite DNA loci in Pacific abalone Haliotis discus discus Mollusca, Gastropoda, Haliotidae. Mol. Ecol. Notes 1, 8-10 https://doi.org/10.1046/j.1471-8278.2000.00004.x
  32. Selvamani, M. J. P., Degnan, S. M. and Degnan, B. M. (2001) Microsatellite genotyping of individual abalone larvae: parentage assignment in aquaculture. Mar. Biotechnol. 3, 478- 485 https://doi.org/10.1007/s1012601-0062-X
  33. Selvamani, M. J. P., Degnan, S. M., Paetkau, D. and Degnan, B. M. (2000) Highly polymorphic microsatellite loci in the Heron Reef population of the tropical abalone Haliotis asinina. Mol. Ecol. 9, 1184-1186 https://doi.org/10.1046/j.1365-294x.2000.00954-9.x
  34. Shephred, S. A. and Brown, L. D. (1993) What is an abalone stock: implication for the role of refugia in conservation. Canadian J. Fish. Aquat. Sci. 50, 2001-2009 https://doi.org/10.1139/f93-224
  35. Singhagraiwan, T. and Doi, M. (1993) Seed production and culture of a tropical abalone, Haliotis asinina Linne. Department of Fisheries, Ministry of Agriculture and Cooperatives
  36. Sweijd, N. A., Bowie, R. C. K., Lopata, A. L., Marinaki, A. M., Harley, E. H. and Cook, P. A. (1998) A PCR technique for forensic, species-level identification of abalone tissue. J. Shellfish Res. 17, 889-895
  37. Tang, S., Tassanakajon, A., Klinbunga, S., Jarayabhand, P. and Menasveta, P. (2005) Population structure of tropical abalone (Haliotis asinina) in coastal waters of Thailand determined using microsatellite analysis. Mar. Biotechnol. (in press)
  38. Tassanakajon, A., Pongsomboon, S., Rimpanitchayakit, V., Jarayabhand, P. and Boosaeng, V. (1997) Random amplified polymorphic DNA (RAPD) markers for determination of genetic variation in wild populations of the black tiger prawn (Penaeus monodon) in Thailand. Mol. Mar. Biol. Biotechnol. 6, 110-115
  39. Walsh, P. S., Metzger, D. A. and Higuchi, R. (1991) Chelex100 as medium for simple extraction of DNA for PCR-based typing from forensic materials. Biotechniques 10, 506-513
  40. Weir, B. S. and Cockerham, C. C. (1984) Estimation F-statistics for the analysis of population structure. Evolution 38, 1358- 1370 https://doi.org/10.2307/2408641
  41. Welsh, J. and McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18, 7213- 7218 https://doi.org/10.1093/nar/18.24.7213
  42. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Res. 18, 6531-6535 https://doi.org/10.1093/nar/18.22.6531
  43. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improve M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mp18 and pUC19. Gene 33, 103-119 https://doi.org/10.1016/0378-1119(85)90120-9

피인용 문헌

  1. Development and application of genomic tools to the restoration of green abalone in southern California vol.15, pp.1, 2014, https://doi.org/10.1007/s10592-013-0524-5
  2. Microsatellite loci in the carcinogenic liver fluke, Opisthorchis viverrini and their application as population genetic markers vol.10, pp.1, 2010, https://doi.org/10.1016/j.meegid.2009.11.005
  3. The systematics and population genetics of Opisthorchis viverrini sensu lato: Implications in parasite epidemiology and bile duct cancer vol.61, pp.1, 2012, https://doi.org/10.1016/j.parint.2011.07.020
  4. Mitochondrial DNA Introgression in the European Abalone Haliotis tuberculata tuberculata: Evidence for Experimental mtDNA Paternal Inheritance and a Natural Hybrid Sequence vol.13, pp.3, 2011, https://doi.org/10.1007/s10126-010-9327-6
  5. Genetic structure of natural populations of California red abalone (Haliotis rufescens) using multiple genetic markers vol.152, pp.6, 2007, https://doi.org/10.1007/s00227-007-0771-4
  6. Genetic population differentiation of the blue swimming crab Portunus pelagicus (Portunidae) in Thai waters revealed by RAPD analysis vol.9, pp.3, 2010, https://doi.org/10.4238/vol9-3gmr886
  7. Utilization of molecular markers for the conservation of blood cockles, Anadara granosa (Arcidae) vol.10, pp.2, 2011, https://doi.org/10.4238/vol10-2gmr1103
  8. Genome-wide characterization of microsatellites and marker development in the carcinogenic liver fluke Clonorchis sinensis vol.114, pp.6, 2015, https://doi.org/10.1007/s00436-015-4419-x
  9. PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade vol.8, pp.1, 2018, https://doi.org/10.1038/s41598-018-30903-x