Molecules and Cells

  • 제19권2호
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  • Pages.185-190
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  • 2005
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Induction of Apoptosis in Chicken Oviduct Cells by C2-Ceramide

  • Kim, Sung Hak (Department of Biology, College of Science, Kyung Hee University) ;
  • Choi, Ji Young (Department of Biology, College of Science, Kyung Hee University) ;
  • Sihn, Choon-Ryoul (Department of Biology, College of Science, Kyung Hee University) ;
  • Suh, Eun Jung (Department of Biology, College of Science, Kyung Hee University) ;
  • Kim, Sun Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Kang Duk (Graduate School of Bio and Information Technology, Hankyong National University) ;
  • Jeon, Iksoo (Laboratory of Transgenic Chicken, Animal Biotechnology Division, National Livestock Research Institute) ;
  • Han, Jae Yong (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Tae-Yoon (Department of Dermato-Immunology, The Catholic University of Korea) ;
  • Kim, Sang Hoon (Department of Biology, College of Science, Kyung Hee University)
  • 투고 : 2004.09.17
  • 심사 : 2004.11.22
  • 발행 : 2005.04.30
⟨ 이전 논문 다음 논문 ⟩

초록

The chicken oviduct is a dynamic organ that produces secretory proteins such as ovalbumin and its cells undergo cell proliferation and differentiation. There has been no study of the cellular mechanism involved in cell death in the chicken oviduct. Therefore, this study has focused on the study of apoptosis in primary oviduct cells. Because ceramide is known to activate apoptosis in tumor cells and is produced in the oviduct, we used an exogenous ceramide analog to induce cell death. The viability of ceramide-treated chicken oviduct cells decreased in a dose-dependent manner and apoptotic cells were detected by staining with annexin V. The expression of apoptosis-related genes was assessed by RT-PCR and bcl-2 mRNA was found to decrease after exposure to ceramide while Bcl-x mRNA increased 12 h post-treatment. In addition, caspase-3 was expressed strongly in the early stages of apoptosis, while caspase-1 and -9 transcripts increased at later times. We conclude that ceramide induces apoptosis in oviduct-derived primary cells via a caspase- and bcl-2-dependent pathway.

키워드

과제정보

연구 과제 주관 기관 : Rural Development Administration

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