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생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Characterization of Ha29, a Specific Gene for Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus

  • Guo, Zhong-Jian (Institute of Applied Entomology, Zhejiang University) ;
  • An, Shi-Heng (Institute of Applied Entomology, Zhejiang University) ;
  • Wang, Dun (Institute of Applied Entomology, Zhejiang University) ;
  • Liu, Yan-He (Institute of Applied Entomology, Zhejiang University) ;
  • Kumar, V. Shyam (Institute of Applied Entomology, Zhejiang University) ;
  • Zhang, Chuan-Xi (Institute of Applied Entomology, Zhejiang University)
  • 발행 : 2005.05.31
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초록

Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (ODVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

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참고문헌

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  1. Characterization of a late expression gene, Open reading frame 128 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus vol.150, pp.12, 2005, https://doi.org/10.1007/s00705-005-0605-y
  2. Analysis of a late gene, orf101 from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus vol.12, pp.5, 2005, https://doi.org/10.1111/j.1005-295X.2005.00041.x
  3. HearSNPV orf83 encodes a late, nonstructural protein with an active chitin-binding domain vol.117, pp.2, 2006, https://doi.org/10.1016/j.virusres.2005.10.019
  4. Biological Comparison of Two Genotypes of Helicoverpa armigera Single-Nucleocapsid Nucleopolyhedrovirus vol.51, pp.6, 2006, https://doi.org/10.1007/s10526-006-9008-6
  5. A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms vol.81, pp.12, 2015, https://doi.org/10.1128/AEM.00339-15