Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Characterization of Ty3-gypsy-like Elements in Hibiscus syriacus

  • Jeung, Ji-Ung (IRRI-Korea Office, NICS, Rural Development Administration) ;
  • Cho, Sung Ki (School of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Seung Jae (School of Life Sciences and Biotechnology, Korea University) ;
  • Shin, Jeong Sheop (School of Life Sciences and Biotechnology, Korea University)
  • Received : 2004.10.18
  • Accepted : 2005.03.02
  • Published : 2005.06.30
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Abstract

Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.

Keywords

References

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