Asian-Australasian Journal of Animal Sciences

  • 제18권1호
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  • Pages.107-112
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  • 2005
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Augmentation of Thermotolerance in Primary Skin Fibroblasts from a Transgenic Pig Overexpressing the Porcine HSP70.2

  • Chen, Ming-Yu (Division of Biotechnology, Animal Technology Institute Taiwan) ;
  • Tu, Ching-Fu (Division of Biotechnology, Animal Technology Institute Taiwan) ;
  • Huang, San-Yuan (Division of Biotechnology, Animal Technology Institute Taiwan) ;
  • Lin, Jyh-Hung (Division of Biotechnology, Animal Technology Institute Taiwan) ;
  • Tzang, Bor-Show (Division of Biotechnology, Animal Technology Institute Taiwan) ;
  • Hseu, Tzong-Hsiung (Department of Life Science, National Tsing-Hua University) ;
  • Lee, Wen-Chuan (Division of Biotechnology, Animal Technology Institute Taiwan)
  • 투고 : 2003.12.19
  • 심사 : 2004.06.02
  • 발행 : 2005.01.01
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초록

A high environmental temperature affects the economic performance of pigs. Heat shock protein 70 (HSP70) has been reported to participate importantly in thermotolerance. This study aims to produce transgenic pigs overexpressing porcine HSP70.2, the highly inducible one of HSP70 members, and to prove the cellular thermotolerance in the primary fibroblasts from the transgenics. A recombinant plasmid in which the sequence that encodes the porcine HSP70.2 gene is fused to green fluorescence protein (GFP) was constructed under the control of cytomegalovirus (CMV) enhancer and promoter. Two transgenic pigs were produced by microinjecting pCMV-HSP70-GFP DNA into the pronucleus of fertilized eggs. Immunoblot assay revealed the varied overexpression level (6.4% and 1.4%) of HSP70-GFP in transgenic pigs. After heating at $45^{\circ}C$ for 3 h, the survival rate (78.1%) of the primary fibroblast cells from the highly expressing transgenic pig exceeded that from the non-transgenic pig (62.9%). This result showed that primary fibroblasts overexpressing HSP70-GFP confer cell thermotolerance. We suggest that transgenic pigs overexpressing HSP70 might improve their thermotolerance in summer and therefore reduce the economic loss in animal production.

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참고문헌

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