Molecules and Cells

  • 제20권2호
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  • Pages.297-302
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  • 2005
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

PLP-1 Binds Nematode Double-stranded Telomeric DNA

  • Im, Seol Hee (Molecular Aging Research Center, Yonsei University) ;
  • Lee, Junho (Molecular Aging Research Center, Yonsei University)
  • 투고 : 2005.04.09
  • 심사 : 2005.06.07
  • 발행 : 2005.10.31
⟨ 이전 논문 다음 논문 ⟩

초록

The integrity and proper functioning of telomeres require association of telomeric DNA sequences with specific binding proteins. We have characterized PLP-1, a $PUR{\alpha}$ homolog encoded by F45E4.2, which we previously identified as a candidate double stranded telomere binding protein, by affinity chromatography followed by mass spectrometry. PLP-1 bound double-stranded telomeric DNA in vitro as shown by competition assays. Core binding was provided by the third and fourth nucleotides of the TTAGGC telomeric repeat. This is quite different from the binding sequence of CEH-37, another C. elegans telomere binding protein, suggesting that multiple proteins may bind nematode telomeric DNA simultaneously in vivo.

키워드

과제정보

연구 과제 주관 기관 : HPEB

참고문헌

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