Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Vibrios Isolated from Diseased Marine Culturing Fishes in Korea

질병의 증상을 보이는 해수 양식 어류에서 분리한 비브리오속 세균

  • Kim, Su-Mi (Institute of Fisheries Science, Pukyong National University) ;
  • Won, Kyung-Mi (Institute of Fisheries Science, Pukyong National University) ;
  • Woo, Sung-Ho (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Li, Hua (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Eun-Jeon (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Choi, Kwang-Jin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Cho, Mi-Young (Pathology Division, NFRDI) ;
  • Kim, Myung-Suk (Pathology Division, NFRDI) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 김수미 (부경대학교 수산과학연구소) ;
  • 원경미 (부경대학교 수산과학연구소) ;
  • 우승호 (부경대학교 수산생명의학과) ;
  • 이화 (부경대학교 수산생명의학과) ;
  • 김은전 (부경대학교 수산생명의학과) ;
  • 최광진 (부경대학교 수산생명의학과) ;
  • 조미영 (국립수산과학원 병리연구팀) ;
  • 김명석 (국립수산과학원 병리연구팀) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20050800
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Abstract

A bacteriological survey in maricultured fish farms was conducted in Korea from 2002 to 2004. A total number of 166 Vibrio isolates were collected from diseased fishes, including olive flounder (133 isolates), black rock fish (8 isolates), red sea bream (6 isolates), shrimp (5 isolates), black sea bream (4 isolates), abalone (3 isolates) and other fishes (7 isolates). All isolates were phenotypically characterized and then groups were obtained using the traditional biochemical test. Representative isolates of each group were genotypically characterized with sequencing the 16S rRNA genes or 16S-23S intergenic space genes. Above 14 species of Vibrio were identified as V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) and others Vibrio sp. (24 strains) by combining of biochemial and genetic characteristics.

본 연구에서는 2002년에서 2004년간 우리 나라 해수 어류 양식장에서 분리되는 세균 중 비브리오 속에 속하는 세균의 종 조성을 조사하였다. 질병의 증상을 보이는 어류로부터 166개의 비브리오속 세균 균주를 수집하였으며, 이들 균주는 넙치 (133 균주), 조피볼락 (8 균주), 참돔 (6 균주), 대하 (5 균주), 감성돔 (4 균주), 전복 (3 균주) 및 기타 해수 어류 (7 균주) 등에서 분리한 균주를 대상으로 하였다. 모든 분리 균주에 대하여 각종 생화학적 성상을 조사하고 그 특성에 따라 분리 균주를 구분하였다. 각 생화화적 성상 group에서 대표 균주를 선정하여 16S rRNA 및 16S-23S rRNA 유전자 서열을 분석하고 이들 결과를 종합하여 분리 균주을 동정하였다. 그 결과 14종 이상의 비브리오 종으로 동정할 수 있었으며, 그 종 조성은 V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) 및 Vibrio spp. (24 strains) 등이었다.

Keywords

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