가미귀비탕(加味歸脾湯)이 생쥐에 Immobilization Stress 부하후(負荷後) 혈중(血中) Corticosterone과 Histamine 함량(含量) 및 면역능(免疫能) 변화(變化)에 미치는 영향(影響)

Effects of Kamikwibitang on Serum Levels of Corticosterone and Histamine and Immune Response after Immobilization Stress in Mice

  • 위석 (원광대학교 한의과대학 부인과학교실) ;
  • 이승준 (원광대학교 한의과대학 부인과학교실) ;
  • 유심근 (원광대학교 한의과대학 부인과학교실)
  • Wi, Seok (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Lee, Seung-Jun (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Yoo, Sim-Keun (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University)
  • 투고 : 2004.10.18
  • 심사 : 2004.11.08
  • 발행 : 2005.02.28

초록

The purpose of this research was to investigate the effects of Kamikwibitang water extract (KKT) on immobilization stress in C57BL/6J mice. KKT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, KKT decreased the cell viability of thymocytes and enhanced the cell viability of splenocytes decreased by immobilization stress. Also, KKT decreased the viability of thymocytes and splenocytes in vitro. KKT decreased DNA fragmentation of splenocytes increased by immobilization stress. KKT decreased the population of thymic $CD4^+CD8^-$ cells increased by immobilization stress, and did not affect the population of $B220^+$ cells and the population of $Thy1^+$ cells changed by immobilization stress and enhanced the population of splenic $CD4^-CD8^+$ cells increased by immobilization stress. KKT enhanced the production of ${\gamma}-interferon$ and did not affect the production of interleukin-2 and interleukin-4 decreased by immobilization stress. Also, KKT decreased the phagocytic activity and the level of nitric oxide decreased by immobilization stress. These results indicate that KKT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of specific-immune response.

키워드

참고문헌

  1. 氏濟生方 嚴用和
  2. 薛氏醫案(文淵閣本 卷1) 薛己
  3. 原色 臨床本草學 辛民敎
  4. 申氏本草學(各論) 申佶求
  5. 婦人良方校注補遺 薛己
  6. 中醫婦科學 羅元愷
  7. 韓方病理學 鄭遇烈
  8. 大韓東醫病理學會誌 v.2 肝機能에 對한 東西醫學的 考察 柳道坤 등
  9. 臟腑辨證論治 金完熙;崔達永
  10. 스트레스와 免疫 v.23 閔炳一(譯)
  11. 精神健康硏究 v.10 스트레스와 精神神經免疫學 강병조
  12. Amer. J. Psychotherapy v.37 Current explorations in Psychoimmunology Udelman, HD;Udelman, DI
  13. Science v.191 Influence of brain and behavior on the immune system Stein, H.;Schiavi, RC.;Comerino, M
  14. 東醫神經精神科學會誌 v.7 no.1 養心湯이 스트레스와 免疫機能에 미치는 影響 尹祥熙 등
  15. 東國韓醫學硏究所論文集 v.9 越鞠丸合補中益氣湯이 電氣刺戟스트레스를 받은 mouse의 免疫機能에 미치는 影響 權泰植 등
  16. 東醫神經精神科學會誌 v.8 no.1 香附子八物湯이 拘束스트레스 흰쥐의 抗스트레스와 免疫反應에 미치는 影響 이승기 등
  17. 大韓韓方婦人科學會誌 v.16 no.3 逍遙散이 생쥐에 Immobilization Stress 負荷 後 血中 免疫能系에 미치는 影響 홍성은 등
  18. 原本 東醫寶鑑(內景篇) 許浚
  19. In methods in Enzymology Endo, Y
  20. J. Biol. Chem. v.231 Zenker, N;Bernstein, DE
  21. Proc. Nat1. Acad. Sci. v.75 Planning for lymphocytes: A method for cell selection. Wysocki, LJ;Sato, VL
  22. J. Immunol. v.120 Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1. Mizel, SB.(et al)
  23. J. Immunol. methods. v.65 Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. Mosmann, T
  24. J. Immunol. methods. v.129 A simple and rapid method to determine hematopoietic growth factor activity. Kotnik, V;Fleischmann, WRJr
  25. J. Immunol. Methods v.139 Rapid and simple method for mesuring thymocyte apoptosis by propidium iodide staining and flow cytometry. Nicoletti, I.(et al)
  26. J. Exp. Med. v.179 Purification and characterization of the Fas-ligand that induces apoptosis. Suda, T;Nagata, S
  27. Arch. Pharm. Res. v.23 no.5 Regulation of cytokine production by exogenous nitric oxide in murine splenocyte and peritoneal macrophage. Eun JS(et al)
  28. J. Immunol. Methods v.174 Chemiluminenscence and nitrite determinations by the MALU macrophage cell line. Boudard, F(et al)
  29. J. Immunol. Methods v.112 Measurement of phagocyte chemiluminenscence in a microtiter plate format. Blair, AL(et al)
  30. J. Immuno. Methods v.162 A rapid and simple microfluorometric phagocytosis assay. Chok PW;Choon SP;Benjamin HS
  31. Infect. Immunity v.59 no.9 Killing of Plasmodium falciparum in vitro by nitric oxide derivatives. Rocket, KA(et al)
  32. 病理學 文濬典 등
  33. 東醫神經精神科學 金相孝
  34. 心身症 黃義完
  35. 서울대 精神醫學報 v.7 視床下部홀몬과 神經傳達物質 金相泰
  36. 新版ストレス 2nd. Ed. 田多井吉之介
  37. Neuroendocrinology v.36 no.6 Central $H_1$- and $H_2$-histaminergic stimulation of pituitary-adrenocortical response under stress in rats. Bugajski J;Gadek A
  38. Nature v.302 no.5906 The vasopressin receptor antagonist dPTyr (Me) AVP does not prevent stress-induced ACTH and corticosterone release. Mormede P
  39. Pathophysiology. v.4 Cold-stress induces thymocyte apoptosis in the rat. Morishita S(et al)
  40. J. Clin. Endocrinol. Metab. v.54 Sridama V;Pacini F;Groot LJ
  41. Life Sci. v.30 no.19 Naloxone enhances stress-induced in noradrenaline turnover in specific brain regions in rats. Tanaka M(et al)
  42. Kor. J. Pharmacogn. v.20 no.1 Effects of Glycyrrhizae Radix on serum corticosterone and blood histamine content by immobilization stress in mice. Eun JS;Oh CH;Han JH
  43. Naturwissenschaften v.83 no.6 Stress and the immune system. Schedlowsk, M;Schmidt RE
  44. Physiol. Behav. v.65 no.1 Differential immune reactivity to stress in BALB/cByJ and C57BL/6J mice: in vivo dependence on macrophages. Shanks N(et al)
  45. Brain Behavior and Immunity. v.1 no.2 Significance of increased secretion of glucocorticoids in mice and rats injected with bacterial endotoxin. Nakano K(et al)
  46. Brain Behav. Immun. v.4 no.4 Stressor-induced alteration of lymphocyte proliferation in mice: evidence for enhancement of mitogenic responsiveness. Lysle DT;Cunnick JE;Rabin BS
  47. Neuroimmunomodulation v.4 no.3 Restraint stress causes tissue-specific changes in the immune cell distribution. Sudo N(et al)
  48. Advances in Immunology v.53 The role of CD4 and CD8 in T cell activation and differentiation. , ; : 59. Miceli MC;Parnes JR
  49. Kor. J. Immunol. v.18 Nitric oxide inhibits macrophage pseudopodia formation in the activated macrophages. Jun, CD(et al)