Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Characteristics of Hydrogel Prepared from Microbial Poly($\gamma$-glutamic acid) by Chemical Crosslinker

  • Park, Jong-Soo (Namyang Dairy Products Co., Ltd.) ;
  • Choi, Seong-Hyun (The Institute of Natural Science, Mokwon University) ;
  • Choi, Woo-Young (Department of Agricultural Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Agricultural Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • Published : 2005.12.31
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Abstract

Microbial hydrogel was prepared with poly(${\gamma}$-glutamic acid) produced from Bacillus subtilis BS62 using crosslinking reagent, ethylene glycol diglycidyl ether (EGDE), and its physico-chemical characteristics were examined. Hydrogel which prepared from 10 grams of 10% PGA solution with $600\;{\mu}l$ of EGDE at $50^{\circ}C$ for 17 h swelled 4,320 times its dry weight, and time to reach swelling equilibrium in deionized water at 4 to $45^{\circ}C$ range was about 20 h. Swollen hydrogel shrunk in ionic solutions, and rate of shrinkage was higher in calcium chloride solution than sodium chloride solution. Swelling rate of hydrogel increased 1.3-fold of initial swelling rate for 30 min at $80^{\circ}C$.

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References

  1. Encyclopedia of Controlled Drug Delivery, vol. 1 Kost, J.;Mathiowitz, E.(ed)
  2. Hydrogels in Medicine and Pharmacy, vol. 1 Peppas, N.A.;Mikes, A.G.
  3. The principles of Polymer Chemistry Flory, P.J.
  4. Macromolecules v.17 Swelling of ionic gels: Quantitative performance of the Donnan theory Ricka, J.;Tanaka, T. https://doi.org/10.1021/ma00142a081
  5. Radiat. Phys. Chem. v.46 Preparation conditions and swelling equilibria of hydrogel prepared by ${\gamma}-irradiation$ from microbial poly(${\gamma}-glutamic$ acid) Choi, H.J.;Kunioka, M. https://doi.org/10.1016/0969-806X(95)00009-M
  6. Macromol. Res. v.13 Preparation and swelling characteristics of hydrogel from microbial poly(${\gamma}-glutamic$ acid) by ${\gamma}-irradiation$ Choi, S.H.;Whang, K.S.;Park, J.S.;Choi, W.Y.;Yoon, M.H. https://doi.org/10.1007/BF03218463
  7. J. Environ. Polymer Degradation v.4 Hydrolytic and enzymatic degradation of poly(${\gamma}-glutamic$ acid) hydrogels and their application in slow-release system for proteins Fan, K.;Gonzales, D.;Sevoian, M. https://doi.org/10.1007/BF02070694
  8. Agric. Chem. Biotechnol. v.47 Production of microbial biopolymer, poly(${\gamma}-glutamic$ acid) by Bacillus subtilis BS 62 Choi, S.H.;Whang, K.S.;Park, J.S.;Choi, W.Y.;Yoon, M.H.
  9. J. Microbiol. Biotechnol. v.11 Enzymatic degradation of poly(${\gamma}-glutamic$ acid) hydrogel prepared by ${\gamma}-ray$ irradiation Hara, T.;Choi, S.H.;Choi, W.Y.
  10. J. Control. Release v.71 Preparation of controlled release systems by free-radical UV polymerizations in the presence of a drug Ward, J.H.;Peppas, N.A. https://doi.org/10.1016/S0168-3659(01)00213-9
  11. J. Appl. Polym. Sci. v.58 Properties of biodegradable hydrogels prepared by ${\gamma}-irradiation$ of microbial poly(${varepsilon}-lysine$) aqueous solutions Kunioka, M.;Choi, H.J. https://doi.org/10.1002/app.1995.070580413
  12. J. Control. Release. v.24 Novel biodegradable hydrogels prepared using the divinylic crosslinking agent N,O-dimethacryloyl hydoxylamine. 1. Synthesis and characterization of rates of gel degradation, and rate of release of model drugs, in vitro and in vivo Ulbrich, K.;Duncan, R. https://doi.org/10.1016/0168-3659(93)90177-7