Surface Characteristics and Adhesive Properties of Pichia farinosa SKM-1, Pichia anomala SKM-T, and Galactomyces geotrichum SJM-59 for Preparation of Probiotics

  • Mo, Eun-Kyoung (Research and Development Center, DBIO Incorporation) ;
  • Lee, Mee-La (Department of Food Science and Biotechnology, Chungnam National University) ;
  • Lee, Sun-Young (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Jae-Cheon (LG Houusehold and Health Care Ltd.) ;
  • Sung, Chang-Keun (Department of Food Science and Biotechnology, Chungnam National University)
  • Published : 2005.08.30

Abstract

Probiotics are generally excreted within a few days if their ingestion in feces at the same rate as or even more quickly than a transit marker (meaning not clear). Ability of probiotics to adhere to intestine prolongs their persistence in gastrointestinal tract, allowing them to exert healthful effects longer. Hydrophobicities, zeta potentials, Alcian blue-binding capacities, and sedimentation profiles of Pichia farinosa SKM-1, P. anomala SKM-T, and Galactomyces geotrichum SJM-59 were determined to evaluate characteristic properties of cell surfaces responsible for adhesion. Results of intestinal Caco-2 cell line in vitro and murine intestine in vivo studies revealed these strains exhibit adhesive properties regardless of their cell surface hydrophobicity.

Keywords

References

  1. J. Appl. Bacteriol. v.66 Probiotics in man and animals Fuller, R.
  2. Trends Food Sci. Technol. v.6 The coming age of probiotics Lee, Y.K.;Salminen, S.
  3. Food Sci. Biotechnol. v.13 Fermented product development of Cyananchum wilfordii hemsley by lactic acid bacteria and Bifidobacteria Moon, J.H.;Om, A.S.;Kwon, H.S.;Kim, Y.J.;Ji, K.E.
  4. Food Sci. Biotechnol. v.13 Identification and probiotic properties of Lactococcus lactis NK 24 isolated from Jeot-gal, a Korean fermented food Lee, N.K.;Kim, T.H.;Choi, S.Y.;Lee, S.K.;Paik, H.D.
  5. Int. J. Food Microbiol. v.44 Demonstration of safety of probiotics Salminen, S.;Wright, A.;Morelli, L.;Marteau, P.;Brassaert, D.;Vos, W.M.;Fond, N.R.;Saxelin, M.;Collins, K.;Mogensen, S.;Birkeland, E.;Sandholm, T.M.
  6. J. Biotechnol. v.68 Safety of industrial lactic acid bacteria Adams, M.R.
  7. Gastroenterol. v.105 Lactobacillus casei GG reverse increased intestinal permeability induced cow milk in suckling rats Isolaurie, E.;Majamaa, H.;Arvola, T.;Rantala, I.;Virtanen, E.;Arvilomi, H.
  8. J. Biotechnol. v.84 Probitic bacteria: safety, functional and technological properties Saalera, M.;Mogensen, G.;Fonden, R.;Matto, J.;Mattila-Sandholm, T.
  9. Characterization and application of three probiotic yeasts form human feces Mo, E.K.
  10. FEBS Lett. v.420 Mannosylphosphate transfer to cell wall mannan is regulated by the transcriptional level of the MNN4 gene in Saccharomyces cerevisiae Odani, T.;Shimma, Y.;Wang, X.H.;Jigami, Y.
  11. Methods Enzymol. v.336 Surface characterization and adhesive properties of Bifido-bacteria Bibiloni, R.;Perez, P.F.;Garrote, G.L.;Disalvo, E.A.;De Antoni, G.L.
  12. Guide for the Care and Use of Laboratory Animals Institute of laboratory Animal Resources
  13. FEMS Microbiol. Lett. v.107 Surface hydrophobic and hydrophilic protein alterations in Candida albicans Hazen, K.C.;Hazen, B.W.
  14. J. Nutr. v.123 AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A rodent diet Reeves, P.G.;Nielson, F.H.;Fahey, G.C.