미생물학회지 (Korean Journal of Microbiology)

  • 제41권4호
  • /
  • Pages.306-311
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  • 2005
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

발광 박테리아 Photobacterium phosphoreum의 Lumazine Protein을 코드 하는 유전자의 염기 서열 분석 및 발현

Generation and Expression of Amino-Terminal Domain of the Gene Coding for the Lumazine Protein from Photobacterium phosphoreum

  • 우영은 (충남대학교 자연과학대학 생화학과) ;
  • 김소영 (충남대학교 자연과학대학 생화학과) ;
  • 이찬용 (충남대학교 자연과학대학 생화학과)
  • Woo Young-Eun (Department of Biochemistry, Chungnam National University) ;
  • Kim So-Young (Department of Biochemistry, Chungnam National University) ;
  • Lee Chan-Yong (Department of Biochemistry, Chungnam National University)
  • 발행 : 2005.12.01
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초록

Lumazine protein은 lux operon의 하류 영역에 존재하는 riboflavin synthase와 아미노산 상동성을 보일 뿐만 아니라, riboflavin synthase의 기질인 6,7-dimethyl-8-ribityllumazine (lumazine)과 결합하여 청록색의 형광을 내게 하는 형광 단백질이다. 발광세균 Photobacterium phosphoreum의 lumazine protein을 코드하는 유전자의 염기서열을 결정하였는데, 이 유전자는 lux operon의 656 bp 상류의 영역에 존재하며, lux operon과는 서로 반대 방향으로 전사되는 것으로 나타났다. 중합효소 연쇄 반응(PCR: Polymerase Chain Reaction)의 방법으로 아미노-말단 절반 lumazine protein을 코드하게 되는 유전자(lumP-N)를 클로닝하여 형질전환의 방법으로 대장균에 유전자를 전이시켜 이들의 유전자의 발현 양상을 조사하여 보았는바, lumP 전체 유전자(lumP-W)가 삽입되어 있는 재조합 플라스미드에서는 발현이 매우 미약한 반면에 아미노 -말단(lumP-N)이 들어있는 경우는 과발현됨을 보였다.

In this study, the amino-terminal half truncated lump and the whole lump genes from Photobacterium phosphoreum coding for the lumazine protein were cloned by polymerase chain reaction and expressed in Escherichia coli. To identifiy of the binding site of the ligand or substrate, the amino acid identities from the sequences of the lumazine protein, yellow fluorescent protein, and riboflavin synthase from different organisms were also compared and analyzed.

키워드

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