Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Cyanobacterial Diversity Analysis Using cpcBA-Intergenic Spacer Region

cpcBA-Intergenic Spacer Region을 이용한 Cyanobacteria의 다양성 분석

  • Choi Gang-Guk (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park Yong-Ha (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn Chi-Yong (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae Myoung-Sook (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh Hee-Mock (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 최강국 (한국생명공학연구원 생물자원센터) ;
  • 박용하 (한국생명공학연구원 생물자원센터) ;
  • 안치용 (한국생명공학연구원 환경생명공학연구실) ;
  • 배명숙 (한국생명공학연구원 환경생명공학연구실) ;
  • 오희목 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2005.12.01
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Abstract

The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified rpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005, The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBA-IGS can be used for the species identification and community analysis of cyanobacteria.

대청호에서 수화 발생이 빈번한 추소리 수역에서 2005년 3월 15일에 채취한 시료를 대상으로 유전자 분식에 의한 cyanobacteria의 다양성을 조사하였다. rpcBA-Intergenic Spacer (IGS)는 cyanobacteria에 특징적 색소인 phycocyanin을 합성하는 유전자와 유전자 사이의 부분으로, 환경시료에서 cyanobacteria의 다양성을 조사하기에 매우 유용한 기능 유전자이다. cpcBA-IGS를 이용하여 restriction fragment length polymorphism (RELP)으로 cyanobacteria의 다양성을 분석한 결과 Phomidium 속은 58 clones, Anabaena 속은 14 clones, Microcyxtis 속은 4 clones, Spirulina 속은 1 clone 그리고 uncultured cyanobacteria 2 clones가 존재하였다. 전반적으로 Phormidium 속이 우점하였으며, 여름철에 수화를 일으키는 Anabaena 속과 Microcystis 속도 많이 분포하였다. 따라서 cyanobacteria는 cpcBA-IGS와 같은 기능 유전자에 의한 종 동정 및 군집분석이 가능함을 보였다.

Keywords

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