Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Monoamine Oxidase Inhibitory Constituents from the Fruits of Cudrania tricuspidata

  • Han Xiang Hua (College of Pharmacy, Chungbuk National University) ;
  • Hong Seong Su (College of Pharmacy, Chungbuk National University) ;
  • Hwang Ji Sang (College of Pharmacy, Chungbuk National University) ;
  • Jeong Seon Hwa (College of Pharmacy, Chungbuk National University) ;
  • Hwang Ji Hye (College of Pharmacy, Chungbuk National University) ;
  • Lee Min Hee (College of Pharmacy, Chungbuk National University) ;
  • Lee Myung Koo (College of Pharmacy, Chungbuk National University, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Dongho (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ro Jai Seup (College of Pharmacy, Chungbuk National University) ;
  • Hwang Bang Yeon (College of Pharmacy, Chungbuk National University)
  • Published : 2005.12.01
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Abstract

A methylene chloride soluble fraction of the fruits of Cudrania tricuspidata significantly inhibited the mouse brain monoamine oxidase (MAO). Three known prenylated isoflavones were isolated and identified by activity-guided fractionation. Gancaonin A (1), 4'-O-methylalpinumisoflavone (2), and alpinumisoflavone (3) inhibited MAO activity in a concentration-dependent manner with $IC_{50}$ values of 19.4, 23.9, and 25.8 $\mu$M, respectively. Of these, gancaonin A (1) showed a selective and potent inhibitory effect against MAO-B ($IC_{50}$ 0.8 $\mu$M) than MAO-A ($IC_{50}$ >800 $\mu$M). The kinetic analysis using Lineweaver-Burk plots indicated that gancaonin A (1) competitively inhibited MAO-B.

Keywords

References

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