Optical Detection of Red Blood Cell Aggregation in a Disposable Microfluidic Channel

  • Shin Sehyun (School of Mechanical Engineering, Kyungpook National University) ;
  • Jang Ju-Hee (School of Mechanical Engineering, Kyungpook National University) ;
  • Park Myung-Soo (School of Mechanical Engineering, Kyungpook National University) ;
  • Ku Yunhee (School of Mechanical Engineering, Kyungpook National University) ;
  • Suh Jang-Soo (Department of Laboratory Medicine, Kyungpook National University Hospital)
  • Published : 2005.03.01

Abstract

The aggregability of red blood cells (RBCs) was determined by laser backscattering light analysis in a microfluidic channel. Available techniques for RBC aggregation often adopt a rotational Couette-flow using a bob-and-cup system for disaggregating RBCs, which causes the system to be complex and expensive. A disposable microfluidic channel and vibration generating mechanism were used in the proposed new detection system for RBC aggregation. Prior to measurement, RBC aggregates in a blood sample were completely disaggregated by the application of vibration-induced shear. With the present apparatus, the aggregation indexes of RBCs can be measured easily with small quantities of a blood sample. The measurements with the present aggregometer were compared with those of LORCA and the results showed a strong correlation between them. The aggregability of the defibrinogenated blood RBCs is markedly lower than that of the normal RBCs. The noble feature of this design is the vibration-induced disaggregation mechanism, which can incorporate the disposable element that holds the blood sample.

Keywords

References

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