Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Production of NO, TNF-α and IL-6 by Squalene, Alkoxy Glycerol, Batyl and Chimyl Solutions in RAW 264.7 Macrophage Cells

RAW 264.7 대식세포에서 NO, TNF-α, IL-6 생산에 관한 Squalene, Alkoxy Glycerol, Batyl, Chimyl의 효과

  • Kim, Young-Ho (College of Pharmacy, Research Institute of Drug Development Chonnam University, Dept of Natural Science, Biology, Chosun University) ;
  • Yoon, Hyun-Joong (College of Pharmacy, Research Institute of Drug Development Chonnam University) ;
  • Moon, Myoung-E (College of Pharmacy, Research Institute of Drug Development Chonnam University) ;
  • Lee, Jun-Haeng (Dept of Natural Science, Biology, Chosun University, Dept of Radiological Science, Nambu University) ;
  • Park, Haeng-Soon (College of Pharmacy, Research Institute of Drug Development Chonnam University) ;
  • Kim, Jong-Se (Dept of Natural Science, Biology, Chosun University)
  • 김영호 (전남대학교 약학대학 약품개발 연구소, 조선대학교 자연과학대학 생물학과) ;
  • 윤현중 (전남대학교 약학대학 약품개발 연구소) ;
  • 문명이 (전남대학교 약학대학 약품개발 연구소) ;
  • 이준행 (조선대학교 자연과학대학 생물학과, 남부대학교 방사선학과) ;
  • 박행순 (전남대학교 약학대학 약품개발 연구소) ;
  • 김종세 (조선대학교 자연과학대학 생물학과)
  • Published : 2005.12.01
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Abstract

The purpose of the present study was to evaluate the effect of Squalene, Alkoxy Glycerol, Batyl and Chimyl solutions on the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF- $\alpha$ ) and interleukin-6 (IL-6) in RAW 264.7 macrophage cells after treatment of C.albicans. The cytotoxic effects was evaluated by the lactate dehydrogenase (LDH). All solutions did not affect on the LDH and NO production by itself. At 6th hour, the TNF- $\alpha$ and IL-6 production was not affected by each solutions. However, at 24th hour, the TNF- $\alpha$ and IL-6 production was affected by itself (p < 0.05). Each solution in the presence of C. albicans decreased the C. albicans-induced TNF- $\alpha$ and IL-6 production compared with C. albicans only (p < 0.05, p < 0.01). These results suggest that Squalene, Alkoxy Glycerol, Batyl and Chimyl solutions will increase the immune response on the C. albicans-induced damage.

본 연구에서는 대식세포인 RAW 264.7 세포주에 SQ ($0.1{\%}$), AG($0.1{\%}$), Batyl(1 mg/mL), Chimyl(1 mg/mL) 용액 등을 단독 혹은 C. albicans와 함께 첨가하여 배양한 후LDH, NO, TNF-$\alpha$, IL-6 발현에 어떤 영향을 주는 지를 관찰하였다. 모든 실험군에서 실험에 사용한 농도는 LDH, NO생산에 영향을 주지 못하였다. 실험에 사용한 용액을 단독 처리한 실험의 경우 TNF-$\alpha$와 IL-6발현의 경우 6시간째에는 큰 차이를 나타내지는 못하였으나, 24시간째에는 발현에 영향을 주었다(p < 0.05). C. albicans을 함께 처리한 실험의 경우에는 C. albicans단독 처리군에 비해 실험 용액을 함께 처리한 군에서 C. albicans유도 TNF-$\alpha$와 IL-6발현을 감소시키는 효과를 관찰하였다(p < 0.05, p < 0.01). 본 연구를 통해 SQ, AG, Batyl, Chimyl 용액이 C. albicans 유도에 의해 감소된 면역반응을 증가시킬 수 있을 것으로 생각되어진다.

Keywords

References

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