Anthocyanin Contents, Effective Components and Botanical Characteristics of the CNU Colored Rice Lines

유색미 CNU 육성계통에 대한 안토시안, 유효성분 및 주요 식물학적 특성

  • Lee Hee-Bong (Department of Agronomy, Chungnam National University) ;
  • Kim Yong-Il (Department of Agronomy, Chungnam National University) ;
  • Park Bo-Young (Department of Agronomy, Chungnam National University) ;
  • Jung Jae-Young (Department of Agronomy, Chungnam National University) ;
  • Song Jae-Young (Department of Agronomy, Chungnam National University) ;
  • Choi Hyun-Gu (Chungnam Agriculture and Extension Service) ;
  • Kang Si-Yong (Korea Atomic Energy Research Institute) ;
  • Kim Bo-Kyoung (National Honam Agricultural Research Institute, RDA)
  • Published : 2005.11.01

Abstract

This study was carried out to evaluate anthocyanine contents, effective components and major botanical characteristics of the new developed colored rices. Characteristics of colored rice lines showed variation in culm length and spikelets per panicle in CNU39, panicle length in CNU43, 1,000 grain weight in CNU90, and yield per plant in CNU119 were highly compared to the Check, Heugjinjubyeo. Relative optical density(ROD) of Heugjinjubyeo was the highest at 530 nm, followed by CNU71, CNU73 and CNU119. The high peaks of ROD of Heugjinjubyeo and CNU71 appeared at 280 nm and 530 nm, and that of CNU43 appeared at 430 nm. The relative anthocyanin contents (RAC) per plant of the CU 71 among the new developed rice compared by yield per unit area was very high, fellowed by CNU73 and Heugjinjubyo.

국내${\cdot}$외에서 수집된 유색미를 분양받아 이들 에 대해 감마선처리 및 수집종간 교배육종 결과 새로 육성한 13개의 공시종에 대해 안토시안 함량, 유효성분 및 주요 수량 관련 형질을 비교 분석하였다. 1. 주요 식물학적 특성으로 대조구인 흑진주벼의 간장은 78cm로 중간형이었고, 개화일수는 다른 공시종에 비해 $7\~20$일 빨랐던 반면에 간장과 수당영화수는 CNU33에서 가장 많았으며, 수장은 CNU 43에서, 천립중은 CNU 90에서 가장 높았으며, 주당 수량은 CNU 119가 가장 높았다. 2. 공시종에 대한 Anthocyanine 색소 함량을 상대흡광도 값 (흡광도$\times$희석배수)으로 비교한 결과, 530nm파장에서 대조구인 흑진주벼가 가장 높게 나타났고, 다음은 CNU71, CNU73, CNU119의 순이었다. 흡광도 값이 가장 높았던 흑진주벼와 CNU71은 280nm와 530nm 부근에서 흡수극대가 나타나 anthocyanin계 색소임이 확인되었고, 흡광도 값이 낮았던 CNU43은 430nm부근에서 흡수극대가 나타나 tannin계 색소로 판명되었다. 3. 공시종에 대한 주당 relative anthocyanin content(RAC)을 비교하기 위해 상대흡광도 값에 주당 현미수량을 곱한 결과 CNU71이 20,112로 가장 높았고, 다음은 CNU73이 16,046 그리고 대조구인 흑진주벼 15,985순으로 나타났다.

Keywords

References

  1. Choi, H.G., J.Y Jung, H.B. Lee, J.T. Jung, and B.K. Kim. 2000. BotanicalCharacteristics for Color Rice Collected. Jour. Agri. Sci.Chungnam Nat'l Univ. 27(2) : 71-80.
  2. Kim, K.H., S.Y Cho, H.P. Moon, and H.C. Choi. 1994. Breeding strategyfor improvement and diversification of grain quality in rice.Korean J. Breed. 26(supplement 2) : 3-18.
  3. Mazza, G. and E. Miniati. 1993. Anthocyanins in fruits, vegetables andgrains. CRC Press. 1-23.
  4. Moon H.P., YG. Choi, J.H. Lee, K.H. Jung, S.Y Cho, H.G. Hwang,K.H. Kang, M.K. Kim, K.H. Hwang, H.C. Choi, and YS. Kim.1998. A new early maturing, anthocyanin pigmented rice varietyHeugjinjubyeo, Korean J. Breed. 30(4) : 67-74.
  5. Nagai, I., G. Suzushino, and Y Tsuboki. 1960. Anthoxanthins andanthocyanins in Orysaceae. I. Japan J. Breed. 10(4) : 47-56.
  6. Osawa, T. 1995. Antioxidative defence systems present in higherplants, and chemistry and function of antioxidative components.Food & Food Ingredients J. Japan 163 : 19-29.
  7. Park, s.z, J.H. Lee, SJ. Han, H.Y. Kim, and S.N. Ryu. 1998. Quantitativeanalysis and varietal difference of cyanidin-3-g1ucoside inpigmented rice. Korean J. Crop Sci. 43(3) : 179-183.
  8. Reddy, V.S., S. Dash, and A.R. Reddy. 1995. Anthocyanin pathway inrice(Orym sativaL.). Theor. Appl, Genet. 91: 301-302.
  9. Ryu, S.N., S.Z. Park, and c.r Ho. 1998. High performance liquidchromatographic determination of anthocyanin pigment in somevarieties of black rice. Journal of Food and Drug Analysis6(4): 729-736.
  10. Strack, D. and V. Wary. 1989. Method in plant biochemistry. Academicpress Ltd. Vo11 : 325-356.
  11. Wang, H., G. Gao, and R.L. Prior. 1997. Oxygen radical absorbingcapacity of anthocyanin. J. Agric. Food chern. 45 : 304-309.
  12. Zhao, Z.S., L.Z. Lai, and J.G. Zheng. 1995. Special rices in China.Shanghai Science & Technol, Publ, Col, China pp. 534.