Plant Resources

The Plant Resources Society of Korea (한국자원식물학회)
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Molecular Cloning of Peroxidase cDNA from Flower Bud of Ginseng(Panax ginseng)

  • In Jun-Gyo (Biopia Co., Ltd.) ;
  • Lee Bum-Soo (Biopia Co., Ltd.) ;
  • Song Won-Seob (College of Agriculture and Life Sciences, Sunchon National University) ;
  • Bae Chang-Hyu (College of Agriculture and Life Sciences, Sunchon National University) ;
  • Choi Seong-Kyu (Department of Oriental Medicine Resources, College of Nature Science, Sunchon National University) ;
  • Yang Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • Published : 2005.08.01
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Abstract

A full-length cDNA (PPrx1) encoding peroxidase has been isolated and its nucleotide sequence determined from flower bud in ginseng plant (Panax ginseng). A PPrx1 cDNA is 1192 nucleotides long and has an open reading frame of 1062 bp with a deduced amino acid sequence of 354 residues (pI 7.53). The deduced amino acid sequence of PPrx1 matched to the previously reported peroxidase protein genes. The PPrx1 showed a high similarity with the $64\%$ identity with peroxidase of N. tabacum (AAK52084). In the phylogenetic analysis based on the amino acid residues, the PPrx1 was closer with peroxidase of G. max (AAD37376).

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