Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Molecular Characterization of a cDNA Encoding Chlorophyll a/b Binding Protein (Cab) from Panax ginseng C. A. Meyer

고려인삼 Chlorophyll a/b Binding Protein(Cab) 유전자의 동정 및 분자적인 특성분석

  • In Jun Gyo (Research Institute of Biotechnology, Biopia Co., Ltd.) ;
  • Lee Bum Soo (Research Institute of Biotechnology, Biopia Co., Ltd.) ;
  • Youn Jae-Ho (Sun Chon University) ;
  • Son Hwa (Dept. of Oriental Medicine Material and Processing, Kyung Hee University) ;
  • Kim Se Young (Dept. of Oriental Medicine Material and Processing, Kyung Hee University) ;
  • Yang Deok Chun (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
  • 인준교 ((주)바이오피아 생명공학연구소) ;
  • 이범수 ((주)바이오피아 생명공학연구소) ;
  • 윤재호 (순천대학교 농업생명과학대학) ;
  • 손화 (경희대학교 한방재료가공학과) ;
  • 김세영 (경희대학교 한방재료가공학과) ;
  • 양덕춘 (경희대학교 한방재료가공학과)
  • Published : 2005.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Photo system II (PSII) is one of the two photosynthetic reaction centers in the chloroplast of higher plants. The chlorophyll a/b-light harvesting complex serves primarily as an antenna for PSII. We isolated a cDNA that encodes a chlorophyll a/b-binding protein (Cab) from Panax ginseng. The small subunit consists of 935 nucleotides long and has an open reading frame of 795 bp with the deduced amino acid of 265 residues (pI 5.63), 28.6 kDa. The deduced amino acid sequence matched to the previously reported Cab genes. Their degree of amino acid identity ranged from 68 to $92\%$. Phylogenetic analysis based on the amino acid residues was showed that the ginseng Cab gene was grouped with P. persica (AAC34983), A. thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), and V. radiate (AAF89205).

광계II(PSII)는 고등식물의 chloroplast에서 두 개의 광합성 반응중심 중의 하나이다. Chlorophyll a/b 광수확 복합체는 광계II를 위한 안테나 역할을 수행한다. 본 연구에서는 인삼의 잎조직을 제작한 cDNA library로부터 chlorophyll a/b-binding protein (Cab) 유전자를 분리하였다. 인삼 Cab유전자는 935 bp의 염기와 265개의 아미노산 잔기(pI 5.63)로 구성된 한 개의 ORF를 포함하고 있으며, 단백질의 분자량은 28.6 kDa으로 추정되었다. 인삼에서 분리한 Cab 유전자는 기존에 식물에서 보고된 유전자들과 유사성을 나타내었으며, 유사도는 $68-92\%$로 나타났다. 아미노산 서열을 비교하여 유연관계를 분석한 결과 인삼의 Cab 유전자는 비교된 P. persica (AAC34983), A.thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), V. radiata (AAF89205) 등과 동일한 그룹으로 분리되었다.

Keywords

References

  1. Altschul, S.F., W. Gish , W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410 https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Babiychuk, E., R.S. Chantz, N. Cherep, J.H. Weil, Y. Gleband and S. Kushnir. 1995. Alterations in chlorophyll a/b binding proteins in Solanaceae cybrids. Mol. Gen. Genet. 249:648-654 https://doi.org/10.1007/BF00418034
  3. Butler, W.L. 1978. Energy distribution in the photochemical apparatus of photosynthesis. Annu. Rev. Plant Physiol. 29:345-378 https://doi.org/10.1146/annurev.pp.29.060178.002021
  4. Cashmore, A.R. 1984. Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide. Proc. Natl. Acad. Sci. USA 81:2960-2964
  5. Dunsmuir, P. 1985. The petunia chlorophyll a/b binding protein genes: a comparison of Cab genes from different gene families. Nuc. Acids Res. 13:2503-2518 https://doi.org/10.1093/nar/13.7.2503
  6. Millar, A.J. and S.A. Kay. 1991. Circadian control of Cab gene transcription and mRNA accumulation in Arabidopsis. The Plant Cell 3: 541-550 https://doi.org/10.2307/3869359
  7. Millar, A.J. and S.A. Kay. 1996. Integration of circadian and phototransduction pathways in the network controlling Cab gene transcription in Arabidopsis. Proc. Natl. Acad. Sci. USA 93: 15491-15496
  8. Morris, P.C., A. Kumar, D.J. Bowles and A.C. Cuming. 1990. Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat. Eur. J. Biochem. 190:625-630 https://doi.org/10.1111/j.1432-1033.1990.tb15618.x
  9. Park, S.W., S.W. Lee, Y.G. Kand, S.W. Lee, C.H. Lee, J.C. Lee and S.Y. Choi. 2001. Expression of Cab (chlorophyll a/b binding protein) gene in transformed plants. Korean J. Plant Tiss. Cult. 28:41-45
  10. Thompson, J.D., D.G. Higgins and T.J. Gilbson. 1994. CLUST AL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-secific gap penalties and weight matrixchoice. Nucleic Acids Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  11. Thornber, J.P. 1975. Chlorophyll-proteins: light harvesting and reaction center components of plants. Annu. Rev. Plant Physiol. 26: 127-158 https://doi.org/10.1146/annurev.pp.26.060175.001015
  12. Thornber, J.P. 1986. In Staehlelin, L.A. and Arnzten, C.J. (eds), Encycl. Plant Physiol. New Series, Vol. 43, Photosynthesis III: Photosynthetic Membranes, Berlin, springer-Verlag.pp. 98-142
  13. Walling, L.L., Y.C. Chang, D.S. Demmin and F.M. Holzer. 1988. Isolation, characterization and evolutionary relatedness of three members from the soybean multigene family encoding chlorophyll a/b binding proteins. Nuc. Acids Res. 16:10477-10492 https://doi.org/10.1093/nar/16.22.10477
  14. Walling, L., G.N. Drews and R.B. Goldberg. 1988. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc. Natl. Acad. Sci. USA 83:2123-2127
  15. Yang, D.C. 1990. Lipid peroxidation of ginseng thy1akoid membrane. Korean J. Ginseng Sci. 14:135-141
  16. Yang, D.C., Q. Chae, S.J Lee, Y.H. Kim and Y.H. Kang. 1990. Effects of light and photosynthetic electron transport system on the generation of singlet oxygen (1O2) in ginseng thylakoid membrane. Korean J. Ginseng Sci. 14:57-62
  17. Yang, D.C., M.W. Kim, Q. Chae and M.S. Kim. 1989. Effects of active oxygen species (1O2, O2-, H2O2) and scavengers on the chlorophyll bleachin of leafburning disease from Panax ginseng C.A. Meyer. Korean J. Ginseng Sci. 13:98-104