Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Shoot Regeneration of Mature Nodal Segments in Poplar (Populus deltoides)

미류나무 성숙 절간조직으로부터 식물체 증식

  • Yang Hee-Sun (Education Department, National Environment Research Institute, Ministry of Environment) ;
  • Kang Hoduck (Forest Biotechnology Lab., Department of Forest Resources, Dongguk University)
  • 양희선 (환경부 국립환경연구원 환경연수부) ;
  • 강호덕 (동국대학교 생명자원과학대학 산림자원학과)
  • Published : 2005.10.01
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Abstract

Mature nodal segments of 2-year-old greenhouse stock plant were cultured on Murashige and Skoog basal medium supplemented with the different kinds and various concentrations of cytokinins to produce multiple shoots in in vitro condition. The most adventitious shoots were produced from excised ends of nodal segments. The highest average number $(24.6\;{\pm}\;4.6)$ of shoots was produced with the combination of BA 1.0mg/L and TDZ 0.1mg/L in MS medium. In addition, several shoots were formed from lenticels of bark cambium with the same treatment. These concentrations promoted high shooting capability upto $94.6\%$ and NAA was the best cytokinin among five different PGR sources.

미류나무 절간조직(2년생)을 이용하여 식물생장조절물질이 함유된 MS배지에서 대량의 식물체를 증식시켰다. 대부분의 식물체는 BA 1.0 + TDZ 0.1 mg/L가 함유한 배지에서 줄기의 형성층 부분에서 절편당 $24.6\;{\pm}\;4.6$개의 줄기가 유기되었으며, 몇몇 줄기는 피목 부분의 조직에서 유기되었다. 신장된 줄기는 NAA 0.01 mg/L가 포함된 배지에서 $94.6\%$의 발근이 이루어졌다. 신장된 줄기의 발근은 식물생장조절물질에 따라 차이를 보여 NAA와 IBA에서 $90\%$ 이상의 발근이 촉진되었으나, cytokinin류를 처리한 시료에서는 $50\%$ 이하의 낮은 발근율을 나타냈다. 발근된 유식 물체는 Cell-tray의 인공상토(peatmoss : vermiculite : perlite = 1 : 1 : 1)에 이식하여 8주간 생육후 $98\%$의 높은 생존율을 나타냈다. 본 연구결과, 2년생 미루나무류의 줄기에서 직접 대량의 줄기를 유도하여 대량증식과정을 단순화 시켰을 뿐만 아니라 조직배양묘 경화시 multi-cap을 이용하여 생존율을 향상시킬 수 있었다.

Keywords

References

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