Journal of Biosystems Engineering

  • 제30권5호
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  • Pages.306-311
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  • 2005
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  • 1738-1266(pISSN)
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  • 2234-1862(eISSN)
한국농업기계학회 (Korean Society for Agricultural Machinery)
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돼지 옴 감염증 진단을 위한 바이오센서 연구

A Biosensor for Diagnosis of Swine Sarcoptic Mange

  • Cho H. K. (Dept. of Biosystems Engineering, Chungbuk National University) ;
  • Jee C. H. (Dept. of Veterinary Medicine, Chungbuk National University)
  • 발행 : 2005.10.01
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초록

In this study, a biosensor was developed to rapidly diagnose the swine sarcoptic mange (Sarcoptes scabies var. suis). The ELISA was modified to reduce the processing time for rapid diagnosis. The biosensor consists of a biological reaction part, and a measurement and control part. The biological reaction part was designed for using micro-pumps and valves for fluid transportation, and the measurement control part composed of a photodiode, a light-emitting diode fur light measurement, and a microcomputer to implement assay A polystyrene covet was used as a reaction chamber. Signal output was read as the rate of change in optical density at 645nm. Eighteen pigs diagnosed with sacroptic mange and 19 control pigs were tested. Fifteen sacbies-infested pigs showed positive results ($83.3\%$ sensitivity). Sixteen control pigs showed negative results ($84.2\%$ specificity). The system could execute a diagnosis cycle in about 45 min. The results suggest that this biosensor is useful for the rapid diagnosis of swine sacroptic mange.

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참고문헌

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