Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

A Study on the Effects of Anticareinogenie Activity of Chondria Crassicaulis

서실 분획물의 암예방효과

  • Jeon Kwang-Hye (Department of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Shin Mi-Ok (Department of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Bae Song-Ja (Department of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
  • 전광혜 (신라대학교 식품영양학과, 마린-바이오 산업지원센터) ;
  • 신미옥 (신라대학교 식품영양학과, 마린-바이오 산업지원센터) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업지원센터)
  • Published : 2005.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study we investigated the biological activity of Chondria Crassicaulis (CC) on the human cancer cells. CC was extracted with methanol and further fractionated into four different types: hexane (CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. We determined the cytotoxic effect of these layers on human cancer cells by MTT assay. Among various partition layers of CC, the CCMM and CCMB showed the strong cytotoxic effects at 150 ${\mu}g$/ml which resulted $98.91\%$, $92.96\%$ on HeLa cell lines and $95.47\%$, $77.05\%$ on MCF-7 cell lines. And, the anti-proliferative effect of CC was accompanied by a marked inhibition of cyclooxygenase (COX-2), Caspase-3 and IAP (cIAP-1, cIAP-2 and XIAP) protein and concomitant induction of p53, p21 and Survivin protein. However, CC did not affect the level of Bax, Bcl-2 and Bcl-XL- protein. Also, we observed quinone reductase (QR) induced effects in all fraction layers of CC on HepG2 cells. The QR induced effects of the CCMH and CCMM on HePG2 cells at 120 ${\mu}g$/mL concentration indicated 3.73 and 2.45 with the control value of 1.0. Although further studies are needed, the present work suggests that CC may be a chemopreventive agent for the treatment of human cancer cells.

Keywords

References

  1. Stavric B. Role of chemopreventers in human diet. Clin Biochem 27: 319, 1994 https://doi.org/10.1016/0009-9120(94)00039-5
  2. Reddy L, Odhav B, Bhoola K. Natural products for cancer prevention: a global perspective. Phannacology & Therapeutics 99: 1-13,2003 https://doi.org/10.1016/S0163-7258(03)00042-1
  3. Doll R. Peto R. The cause of cancer. Quantitative estimates of available risks of cancer in the United States today. J Natl Cancer Inst 66: 1192-1308, 1981
  4. Schwartsmann G,ha AB, Berlinck R, GS, Jimeno J. Marine organisms as a souce of new anticancer agents. Oncology 2: 221-225,2001 https://doi.org/10.1159/000214620
  5. Krinsky Nl. Effects of carotenoids in celluar and animal systems. Am J Clin Nutr 53: 38S-246S, 1991
  6. Dunlap WC, YAmamoto Y. molecule antioxidants in marine organism: antioxidant activity of mycosporine-glycine. Comp Biochem Physiol 112B: 105-114,1995 https://doi.org/10.1016/0305-0491(95)00086-N
  7. Ryu BH, Kim DS, Cho KJ, Sin DB. Antitumor activity of seaweeds toward sarcoma-180. Koran J Food Sci Technol 21 (5): 595-600,1989
  8. Park YB, Kim IS, Yoo SJ, Ahn JK., Lee TG, Park DC, Kim SB. Elucidation of anti-tumor initiator and promoter derived from seaweed-2: investigation of seaweed extracts suppressing mutagenic activity of PhIP and MeIQx. J Korean Fish Soc 31(4): 581-586,1998
  9. Park YB, Kim IS, Yoo SJ, Ahn JK., Lee TG, Park DC, Kim SB. Elucidation of anti-tumor initiator and promoter derived from seaweed-3: anti-tumor promoters of Ecklonia stolonifera extracts. J Korean Fish Soc 31 (4): 587-593, 1998
  10. Talalay P, Benson AM. Elvation of quinone reductase activity by anticarcinogenic antioxidants. Advances in Enzyme Regulation 20: 287-300, 1982 https://doi.org/10.1016/0065-2571(82)90021-8
  11. Wefers H, Komai T, Talalay P, Sies H. Protection against reactive oxygen species by NAD(P) H: quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Federation of European Biochemical Societies 169 (1): 63-66, 1984
  12. Steinkellner H, Rabot S, Freywald C, Nobis E, Scharf G, Chabicovsky M, Knasmiiller S, Kassie F. Effects of c rucifrous vegetable and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutation Research 480-481: 285-297, 2001
  13. Shon YH, Nam K. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. Journal of Ethnopha-macology 77: 103-109,2001
  14. Gerh?user C, Klimo K, Heiss E, Neumann I, Gamal-Eldeen A, Knauft J, Liu GY, Sitthimonchai S, Frank N. Mechanism-based in vitro screening of potential cancer chemopreventive agents. Mutation Research 523-524: 163-172,2003 https://doi.org/10.1016/S0027-5107(02)00332-9
  15. Shim SM, Kim MH, Bae SJ. Cytotoxicity and quinone reductase induced effects of Daucus carota L. leaf extracts on human cancer cells. J Korean Soc Food Sci Nutr 30(1): 86-91, 2001
  16. Bae SJ. The effects of anticarcinogenic activity of Solanum tuberosum peel fractions. J Korean Soc Food Sci Nutr 31 (5) : 905-909,2002
  17. Lee YP, Yoon SY. Taxonomy of Chondria (Rhodopfyta) in Korea Algae. Korean J Phycology 11 (1): 107-139, 1996
  18. Edmonds JS, Shibata Y, Yang F, Morita M. Isolation and synthesis of I-deoxy-l-dimethylarsinoylribitol-5-sulfate, a natural constituent of Condria crassicaulis and other red algae. Tetrahedron Letters 38 (33): 5819-5820, 1997 https://doi.org/10.1016/S0040-4039(97)01330-0
  19. Park JH, Kang KC, Baek SB, Lee YH, Rhee KS. Separation of antioxidant compounds from edible marine algae. Koran J Food Sci Technol 23 (3) : 256-260, 1991
  20. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbortt BJ, Mayo JG, Shoemaker RH, Boyd MR. Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601, 1988
  21. Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. Evalution of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942, 1987
  22. Prochaska HJ, Santamaria AB. Direct measurement of NAD(P) H: Quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme in ducers. Anal Biochem 169: 328-336, 1988 https://doi.org/10.1016/0003-2697(88)90292-8
  23. Prochaska HJ. Screening strtegies for the detection of anticarcinogenic enzyme inducers. J Nutr Biochem 5: 360, 1994 https://doi.org/10.1016/0955-2863(94)90067-1
  24. Lee JH, Lee E, Park J, Kim E, Kim J. In vivo p53 function is indispensable for DNA damage-induced apoptotic signaling in Drosophlia. FEBS Letters 550: 5-10,2003 https://doi.org/10.1016/S0014-5793(03)00771-3
  25. Subbaramaiah K, Altorki N, Chung WJ, Mestre JR, Sampat A, Dannenberg AJ. Inhibition of cyclooxygenase-2 gene expression by p53. J Bioi Chem 274: 10911-10915, 1999 https://doi.org/10.1074/jbc.274.16.10911
  26. Min SH, Shon YH, Park CH, Lee WS, Joo YE, Choi SK, Rew JS, Park CS, Kim SJ. Expression of cyclooxygenase-2 and p53 in gastric cancer and precursor lesions. Korean J Gastroenterology 41(4):268-276,2003
  27. Smali A, Zhivotoxvsky B, Jones D, Nagata S, Orrenius S. Apoptpsis: cell death defined by caspase activation. Cell Death Differ 6: 495-496, 1999 https://doi.org/10.1038/sj.cdd.4400520
  28. Baliga BC, Kumar S. Role of Bcl-2 family of proteins in malignancy. Hematol Oncol 20 (2) : 63-74,,2002 https://doi.org/10.1002/hon.685
  29. Lee HM, Kim YJ, Park TS. Induction of Apoptoisis by (-)epigallocatechin- 3-gallate in HL-6O Cells. J Korean Soc Food Sci Nutr 36 (4) : 382-388,2003
  30. Femandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-l beta-converting enzyme. J Bioi Chem 269: 30761-30764,1994
  31. Yu J, Leung WK, Ebert MP, et aI. Increased expression of survivin in gastric cancer patients and in first degree relative. Br J Cancer 87: 91-97, 2002 https://doi.org/10.1038/sj.bjc.6600421
  32. Shim SM, Choi SW, Bae SJ. Effets of Punica granatum L. Fraction on quinone reductase induction and growth inhibition on several cancer cells. J Korean Soc Food Sci Nutr 30(1): 8085,2001
  33. Bae SJ. Studies on the antioxidative and antimicrobial effects of Chondria crassicaulis. J Life Sci 14 (3) : 411-416, 2004 https://doi.org/10.5352/JLS.2004.14.3.411