Macromolecular Research

  • 제13권5호
  • /
  • Pages.446-452
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  • 2005
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  • 1598-5032(pISSN)
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  • 2092-7673(eISSN)
한국고분자학회 (The Polymer Society of Korea)
권호 표지

Surface Characteristics and Fibroblast Adhesion Behavior of RGD-Immobilized Biodegradable PLLA Films

  • Jung Hyun Jung (Biomaterials Research Center, Korea Institute of Science and Technology) ;
  • Ahn Kwang-Duk (Biomaterials Research Center, Korea Institute of Science and Technology) ;
  • Han Dong Keun (Biomaterials Research Center, Korea Institute of Science and Technology) ;
  • Ahn Dong-June (Department of Chemical and Biological Engineering, Korea University)
  • 발행 : 2005.10.01
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초록

The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLA-g-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/$cm^2$. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control

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