Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Optimization of a Diagnostic DNA Chip for Fish Rhabdovirus

  • Kim Young Ju (Department of Microbiology, Pukyong National University) ;
  • Kang Ji Hee (Department of Microbiology, Pukyong National University) ;
  • Kim Su Mi (Department of Fish Life Medicine, Pukyong National University) ;
  • Park Soo Il (Department of Fish Life Medicine, Pukyong National University) ;
  • Kim Sang Bong (Department of Mechatronics, Pukyong National University) ;
  • Lee Myung Suk (Department of Microbiology, Pukyong National University)
  • Published : 2005.09.01
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Abstract

A DNA chip that rapidly and accurately detects the viral genes in rhabdovirus-infected fish was developed. The N, Ml, and G proteins of three rhabdovirus strains, infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), and flounder rhabdovirus (HIRRV), were selected for use as probes. The sequences of the corresponding genes were obtained, and probes were prepared by PCR using specific primer sets. The specificity of the probes was confirmed by cross PCR. The prepared probes were spotted on poly-L-lysine- or aminosilane-coated glass slides and hybridized with target DNA under several different conditions in order to determine the optimal hybridization temperature, glass-slide coating, and target cDNA concentration.

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References

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Cited by

  1. Detection of Fish Rhabdoviruses using a Diagnostic Fish Rhabdovirus DNA Chip vol.8, pp.3, 2005, https://doi.org/10.5657/fas.2005.8.3.185