Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of Biosurfactant from Bacillus atrophaeus DYL,-130

Bacillus atrophaeus DYL-130이 생산하는 biosurfactant의 분리 및 특성

  • Kim Sun-Hee (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University) ;
  • Lee Sang-Cheol (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University) ;
  • Park In-Hye (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University) ;
  • Yoo Ju-Soon (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University) ;
  • Joo Woo-Hong (Dept. of biology, Chang Won University) ;
  • Hwang Cher-Won (Institute of Bioscience and Technology, Handong Global University) ;
  • Choi Young-Lark (Department of Biotechnology, Faculty of Natural Resources and Life Science Dong-A University)
  • 김선희 (동아대학교 응용생명공학부) ;
  • 이상철 (동아대학교 용용생명공학부) ;
  • 박인혜 (동아대학교 응용생명공학부) ;
  • 유주순 (동아대학교 응용생명공학부) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 황철원 (한동대학교 생명공학 연구소) ;
  • 최용락 (동아대학교 응용생명공학부)
  • Published : 2005.10.01
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Abstract

The objective of this study was investigate the characteristic of biosurfactant produced from the iso-lated strain. The strain was isolated from soli samples of Duck-Yu Mountain and it was identified as Bacillus atrophaeus DYL-130 by 16S rDNA and gyrA gene nucleotide sequence analysis. The surface ten-sion of culture filtrate of Bacillus atrophaeus DYL-130 decreased to 28 mN/m and its biosurfactant con-centration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The emulsifying activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oils as substrate. The biosurfactant was purified by affinity chromatography and the surface activity of purified biosurfactant was measured by drop-collapsing method and it could be effectively emulsify toluene.

원유 분해능이 강력한 균주를 얻고자 덕유산의 토양으로부터 crude oil을 탄소원으로 이용하는 수십 종을 분리하였다. 분리된 균주 중 원유분해능 및 biosurfactant 생성능이 우수한 균주를 선별하여, 형태학적 특성을 관찰하고 165 rDNA sequence와 』gyrA gene sequence를 통하여 Bacillus atrophaeus DYL-130으로 동정하였다. 동정된 균주 배양액의 표면장력은 최저 28 mN/m까지 감소되었다. 또한 Bacillus atrophaeus DYL-130이 생산하는 biosur-factant가 column chromatography에 의하여 분리 되었으며, 분리된 biosurfactant의 toluene 유화능 및 crude biosurfactant의 유화활성과 안정성이 시험 되었다. Crude biosurfactant의 유화활성은 kerosene에서 최대였으며, soybean oil에서도 높은 편이였으나 유화안정성의 경우 soybean oil을 기질로 하였을 경우가 kerosene을 기질로 하였을 때 보다 우수하였다. 또한 crude biosurfactant의 유화안정성을 합성계면활성제와 비교 한 결과 DYL-130이 생산하는 biosurfactant의 유화안정성이 합성계면활성제와 유사하거나 뛰어남을 확인하였다. Column chroma-tography에 의하여 분리된 biosurfactant는 drop-collapsing method에 의하여 surface-activity가 확인 되었으며 또한 toluene에 대한 유화력이 매우 뛰어난 것을 확인 하였다. 따라서 DYL-130에서 추출한 biosurfactant는 합성계면활성제를 대체할 수 있는 환경친화적인 생물 계면활성제로 사용될 수 있는 가능성을 보여주고 있다. 산업적으로 이 물질을 이용하기 위해서 Bacillus atrephaeus DYL-130이 생산하는 biosurfactant에 대한 구조분석과 물리 화학적 특성, 생분해도 및 환경독성 등의 조사를 수행하여야 할 것으로 생각된다.

Keywords

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