Journal of Microbiology and Biotechnology

  • 제15권5호
  • /
  • Pages.946-951
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  • 2005
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Kinetics of di-n-Butyl Phthalate Degradation by a Bacterium Isolated from Mangrove Sediment

  • XU XIANG-RONG (Laboratory of Environmental Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong) ;
  • GU JI-DONG (Laboratory of Environmental Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong, The Swire Institute of Marine Science, The University of Hong Kong) ;
  • LI HUA-BIN (Laboratory of Environmental Toxicology, Department of Ecology & Biodiversity, The University of Hong Kong) ;
  • LI XIAO-YAN (Laboratory of Environmental Engineering, Department of Civil Engineering, The University of Hong Kong)
  • 발행 : 2005.10.01
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초록

Biodegradation of the endocrine-disrupting chemical di-n-butyl phthalate (DBP) was investigated using a bacterium, Pseudomonas fluorescens B-1, isolated from mangrove sediment. The effects of temperature, pH, salinity, and oxygen availability on DBP degradation were studied. Degradation of DBP was monitored by solid-phase extraction using reversed-phase HPLC and UV detection. The major metabolites of DBP degradation were identified as mono-n-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry (GC-MS) and a pathway of degradation was proposed. Degradation by P. fluorescens B-1 conformed to first-order kinetics. Degradation of DBP was also tested in seawater by inoculating P. fluorescens B-1, and complete degradation of an initial concentration of $100{\mu}g/l$ was achieved in 144 h. These results suggest that DBP is readily degraded by bacteria in natural environments.

키워드

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