Ethaboxam의 배추 뿌리혹병 방제효과

Control Efficacy of Ethaboxam on Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae

  • 최경자 (한국화학연구원 생물기능연구팀) ;
  • 장경수 (한국화학연구원 생물기능연구팀) ;
  • 김진철 (한국화학연구원 생물기능연구팀) ;
  • 임희경 (한국화학연구원 생물기능연구팀) ;
  • 전삼재 (LG생명과학기술연구원 정밀화학연구그룹) ;
  • 김달수 (LG생명과학기술연구원 정밀화학연구그룹) ;
  • 조광연 (한국화학연구원 생물기능연구팀)
  • Choi, Gyung-Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lim, He-Kyoung (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Chun, Sam-Jae (Specialty Chemicals Research Group, LG Life Sciences Ltd. R&D Park) ;
  • Kim, Dal-Soo (Specialty Chemicals Research Group, LG Life Sciences Ltd. R&D Park) ;
  • Cho, Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology)
  • 발행 : 2005.03.31

초록

Ethaboxam[(RS)-N-(a-cyano-2-thenyl)-4-ethyl-2-(ethylamino)-1,3-thiazole-5-carboximide]은 난균강 미생물에 대하여 우수한 살균활성을 보이는 신규 살균제이다. Ethaboxam 원제와 몇 가지 ethaboxam 제형의 배추 뿌리혹병에 대한 방제효과를 조사하였다. Ethaboxam을 관주처리 하였을 때, 토양 1 L 당 8.33 mg 농도 처리구는 배추 뿌리의 혹 형성이 완전히 억제되었으며, $EC_{50}$ 값은 2.65 mg/L 토양이었다. Ethaboxam 5개 제형(10% 액상수화제, 15% 액상수화제, 2% 입제, 5% 입제, 25% 수화제) 그리고 ethaboxam과 metalaxyl 합제 제형(3%+1% 입제)의 배추 뿌리혹병 방제효과는 우수하였다. 이들 중 토양 관주처리 한 ethaboxam의 10% 및 15% 액상수화제, 토양 혼화처리 한 ethaboxam 2% 입제 제형은 다른 제형보다 배추 뿌리혹병에 대하여 더 우수한 방제효과를 보였다. 이들의 배추 뿌리혹병에 대한 $EC_{50}$ 값은 원제기준으로 토양 L 당 각각 3.72 mg(10% 액상수화제), 1.10 mg (15% 액상수화제), 4.95 mg (2% 입제) 이었다. 그러므로 실험한 ethaboxam 6개 제형 중에 15% 액상수화제가 배추 뿌리혹병에 대하여 가장 우수한 방제효과를 나타냄을 알 수 있었다. 이들 결과로부터 ethaboxam은 포장에서도 배추 뿌리혹병을 효과적으로 방제할 수 있으리라 생각되었다.

Ethaboxam[(RS)-N-(a-cyano-2-thenyl)-4-ethyl-2-(ethylamino)-1,3-thiazole-5-carboximide] is a novel fungicide with high level of activity against Oomycetes fungi. The control effects of ethaboxam technical and various ethaboxam formulations were investigated against P. brassicae, the causal agent of clubroot disease in Chinese cabbage. When ethaboxam was applied to infested soil, club formation caused by P. brassicae was strongly inhibited at 8.33 mg/L soil and $EC_{50}$ of ethaboxam was 2.65 mg/L soil. Five ethaboxam formulations [10% suspension concentrate (SC), 15% SC, 2% granule (GR), 5% GR, 25% wettable powder] and mixture formulation of ethaboxam and metalaxyl (3%+1% GR) exhibited good efficacy against the pathogen. 10% SC, 15% SC, and 2% GR formulations of ethaboxam showed better disease controlling efficacy on Chinese cabbage clubroot than the other formulations. The $EC_{50}$ values of 10% SC, 15% SC, and 2% GR formulations of ethaboxam were 3.72 mg AI/L soil, 1.1 mg AI/L soil, and 4.95 mg AI/L soil, respectively. Among them, soil drenching application by 15% SC formulation of ethaboxam exhibited the most in vivo antifungal activity on P. brassicae. These results indicate that ethaboxam has a high potential for the control of clubroot disease.

키워드

참고문헌

  1. Arie, T., Y. Kobayashi, G. Okada, Y. Kono and I. Yamaguchi (1998) Control of soilborne clubroot disease of cruciferous plants by epoxydon from Phoma glomerata. Plant Pathology 47:743-748 https://doi.org/10.1046/j.1365-3059.1998.00298.x
  2. Braselton, J. (1995) Current status of plasmodiophorids. Crit. Rev. Microbiology 21:263-275 https://doi.org/10.3109/10408419509113543
  3. Daggett, S. S., E. Gotz and C. D. Therrien (1993) Phenotypic changes in populations of Phytophthora infestans populations from Eastern Germany. Phytopathology 83:319-323 https://doi.org/10.1094/Phyto-83-319
  4. Davidse, L. C., D. Looijen, L. Turkensteen and D. van der Wal (1981) Occurrence of metalaxyl-resistant strains of Phytophthora infestans in Dutch potato fields. Neth. J. Plant Pathol. 87:65-68 https://doi.org/10.1007/BF01976658
  5. Dowley, L. J. and E. O'Sullivan (1981) Metalaxyl resistant strains of Phytophthora infestans (Mont.) de Bary in Ireland. Potato research 24:417-421 https://doi.org/10.1007/BF02357324
  6. Hawksworth, D. L., P. M. Kirk, B. C. Sutton and D. N. Pegler (2001) Anisworth & Bisby's Dictionary of the Fungi. 9th ed., 655p., CAB Internatinal, UK
  7. Ikegami, H. (1975) Susceptibility and resistance to clubroot of the cruciferous vegetable species and varieties. Studies on the clubroot of cruciferous plant 1. Res. Bull. Fac. Agric. Gifu Univ. 38: 11-28
  8. Kim, B. S., X. Z. Xhang, E. K. Chung, D. S. Kim, S. J. Chun and W. B. Choi (2003) Sensitivity of Phytophthora irifestans isolates to fungicides metalaxyl and ethaboxam in Korea. Plant Pathol. J. 19:143-147 https://doi.org/10.5423/PPJ.2003.19.3.143
  9. Kim, D. S., S.-J Chun, J.-J. Jeon, S.-W. Lee and G.-H. Joe (2004) Synthesis and fungicidal activity of ethaboxam against Oomycetes. Pest Manag. Sci. 60: 1007-1012 https://doi.org/10.1002/ps.873
  10. Kim, D. S., Y. S. Lee, S. J. Chun, W. B. Choi, S. W. Lee, G. T. Kim, K. G. Kang, G. H. Joe and J. H. Cho (2002) Ethaboxam: a new Oomycetes fungicide. Proc. Br. Crop Prot. Conf.-Pests and Dis. 1:377-382
  11. Kim, D. S., H. C. Park, S. J. Chun, S. H. Yu, K. J. Choi, J. H. Oh, K. H. Shin, Y. J. Ko, B. S. Kim, Y. I. Hahm and B. K. Chung (1999) Field performance of a new fungicide, ethaboxam, against cucumber downy mildew, potato late blight and pepper Phytophthora blight in Korea. Plant Pathol. J. 15:48-52
  12. Komyoji, T., K. Sugimoto, S. Mitani, N. Matsuo and K. Suzuki (1995) Biological properties of a new fungicide, fluazinam. J. Pesticide Sci. 20:129-135 https://doi.org/10.1584/jpestics.20.129
  13. Lorenz, D. H. and K. W. Eichhorn (1982) Botrytis cinerea and its resistance to dicarboximide fungicides. EPPO Bull. 12:125-129 https://doi.org/10.1111/j.1365-2338.1982.tb01692.x
  14. Mitani, S., K. Sugimoto, H. Hayashi, Y. Takii, T. Ohshima and N. Matsuo (2003) Effects of cyazofamid against Plasmodiophora brassicae Woronin on Chinese cabbage. Pest Manag. Sci. 59:287-293 https://doi.org/10.1002/ps.627
  15. Mitani, S., S. Araki, T. Yamaguchi, Y. Takii, T. Ohshima and N. Matsuo. (2001a) Antifungal activity of the novel fungicide cyazofamid against Phytophthora infestans and other plant athogenic fungi in vitro. Pestic. Biochem. Physiol. 70:92-99 https://doi.org/10.1006/pest.2001.2541
  16. Mitani S., S. Araki, Y. Takii, T. Ohshima, N. Matsuo and H. Miyoshi (2001b) The biochemical mode of action of the novel selective fungicide cyazofamid: Specific inhibition of mitochondrial complex III in Pythium spinosum. Pestic. Biochem. Physiol. 71: 107-115 https://doi.org/10.1006/pest.2001.2569
  17. Shimotori, H., H. Yanagida, Y. Enomoto, K. Igarashi, M. Yoshinari and M. Umemoto (1996) Evaluation of benzenesulfonanilide derivatives for the control of crucifers clubroot. J. Pesticide Sci. 21:31-35 https://doi.org/10.1584/jpestics.21.31
  18. Stanghellini, M. E. and R. M. Miller (1997) Their identity and potential efficacy in the biological control of zoosporic plant pathogens. Plant Dis. 81:4-12 https://doi.org/10.1094/PDIS.1997.81.1.4
  19. Tanaka S., S. Yoshihara, S. Ito and M. Kameya-Iwaki (1997) The influence of virulence of Plasmodiophora brassicae populations on epidemiology of Chinese cabbage clubroot and efficacy of fungicides. Ann. Phytopathol. Soc. Jpn. 63:183-187 https://doi.org/10.3186/jjphytopath.63.183
  20. Williams, P. H. (1966) A system for the determination of the races of Plasmodiophora brassicae that infest cabbage and rutabaga. Phytopathology 56:624-626
  21. Yoshigawa, H. (1983) Breeding for clubroot resistance of crucifer crop in Japan. Jpn. Agr. Res. Quart. 17: 6-11
  22. Yoshikawa, H. and S. T. Buczacki (1978) Clubroot in Japan: research and problems. Rev. Plant Pathol. 57:253-257
  23. 김두욱, 오정행 (1997) 배추 무사마귀병의 발생상황과 병원균(Plasmodiophora brassicae)의 병원성 및 배추 품종의 병저항성. 한국식물병리학회지 13:95-99
  24. 김충회, 조원대, 김홍모 (2000a) 배추무사마귀병균의 토양내 분포. 식물병 연구 6(1):27-33
  25. 김충회, 조원대, 김홍모 (2000b) 배추무사마귀병균 휴면포자의 발아 및 생존에 미치는 몇가지 환경요인. 한국농약과학회지 4(4):66-71
  26. 조광수, 한영환, 이정태, 허은주, 양태진, 우종규 (2002) 고랭지 지역 배추 무사마귀병원균의 생리형 분화와 저항성 품종 선발. 한국육종학회지 34:168-173
  27. 오정행,조장환, 김봉구, 채제천, 정길웅, 황철호, 김두욱 (1997) 배추 무사마귀병(Plasmodiophora brassicae)의 발병 유인 및 약제방제. 한국식물병리학회지 13:244-247
  28. 최경자, 김병섭, 정영륜, 조광연 (1992) 감자 재배포장에서 Metalaxyl 저항성인 감자역병균(Phytophthora infestans)의 발생. 한국식물병리학회지 8(1):34-40