Fungicide Screening for Control of Summer Spinach Damping-off Caused by Rhizoctonia solani

Rhizoctonia solani에 의한 여름 시금치 잘록병의 방제를 위한 살균제 선발

  • Kim, Byung-Sup (Department of Applied Plant Science, Kangnung National University) ;
  • Yun, Yue-Sun (Department of Applied Plant Science, Kangnung National University) ;
  • Yun, Choel-Soo (Department of Applied Plant Science, Kangnung National University) ;
  • Zhang, Xuan-Zhe (Department of Applied Plant Science, Kangnung National University) ;
  • Yeoung, Young-Rog (Department of Applied Plant Science, Kangnung National University) ;
  • Hong, Sae-Jin (Department of Applied Plant Science, Kangnung National University)
  • 김병섭 (강릉대학교 식물응용과학과) ;
  • 윤여순 (강릉대학교 식물응용과학과) ;
  • 윤철수 (강릉대학교 식물응용과학과) ;
  • 장현철 (강릉대학교 식물응용과학과) ;
  • 용영록 (강릉대학교 식물응용과학과) ;
  • 홍세진 (강릉대학교 식물응용과학과)
  • Published : 2005.03.31

Abstract

Damping-off of summer spinach caused by Rhizoctonia solani AG-4 has become a very important disease. For the control of summer spinach damping-off, antifungal activity of thirteen fungicides (pencycuron, trifloxystrobin, pyraclostrobin, azoxystrobin, kresoxim-methyl, validamycin, fluazinam, Benlate-T, flutolanil, cyazofamid, hexaconazole, tebuconazole, prochloraz) were evaluated in vitro and in vivo. Pencycuron, pyraclostrobin, validamycin, fluazinam, Benlate-T, hexaconazole, tebuconazole, and flutolanil significantly suppressed the mycelial growth of the pathogenic fungus. However, trifloxystrobin, azoxystrobia kresoxim-methyl, cyazofamid, and prochloraz did not represent good inhibition on the growth of R. solani. When applied by soil drenching (2,000 mg/L), pencycuron, pyraclostrobin, validamycin, fluazinam, Benlate-T, and flutolanil provided spinach survival ratios of 97.8%, 84.4%, 93.3%, 95.6%, 91.1%, and 86.7%, respectively. Also when treated in seed at 2,000 ing/L, pencycuron and pyraclostrobin displayed survival ratios of more than 85.1%.

Rhizoctonia solani AG-4에 의하여 발생하는 여름 시금치 잘록병은 매우 중요한 병이다. 이 병을 방제하기 위하여 13종(pencycuron, trifloxystrobin, pyraclostrobin, azoxystrobin, kresoxim-methyl, validamycin, fluazinam, Benlate-T, flutolanil, cyazofamid, hexaconazole, tebuconazole, prochloraz)의 살균제의 약효를 in vitro와 in vivo 검정을 통하여 평가하였다. In vitro 균사생장 억제 시험 결과 pencycuron, pyraclostrobin, validamycin, fluazinam, Benlate-T, hexaconazole, tebuconazole, flutolanil이 병원균의 생장을 크게 억제하였다. 그러나 trifloxystrobin, azoxystrobin, kresoxim-methyl, cyazofamid, prochloraz은 억제 정도가 낮게 나타났다. 살균제들을 관구처리한 유묘검정(2,000 mg/L)에서 pencycuron, pyraclostrobin, validamycin, fluazinam, Benlate-T, flutolanil은 97.8%, 84.4%, 93.3%, 95.6%, 91.1%, 86.7%의 생존율을 나타냈고, 2,000 mg/L의 농도로 종자처리하였을 때 pencycuron과 pyraclostrobin은 85.1% 이상의 생존율을 나타내었다.

Keywords

References

  1. Ammermann, E., G. Lorenz, K. Schelberger, B. Mueller, R. Kirstgen and H. Sauter (2000) BAS 500 F-the new broad-spectrum strobilurin fungicide. Proceedings of the BCPC Conference on Pests and Diseases, BCPC, Farnham, Surrey, UK, 2, pp.541-548
  2. Barnes J. S. and A. S. Csinos (1990) Effects of fungicides, cultivars, irrigation, and environment on Rhizoctonia limb rot of peanut. Plant Dis. 74:671-676 https://doi.org/10.1094/PD-74-0671
  3. Bartlett, D. W., J. M. Clough, J. R. Godwin, A. A. Hall, M. Hamer and B. Parr-Dobrzanski (2002) The strobilurin fungicides. Pest Manag. Sci. 58:649-662 https://doi.org/10.1002/ps.520
  4. Campion C., C. Chatot, B. Perraton and D. Andrivon (2003) Anastomosis groups, pathogenicity and sensitivity to fungicides of Rhizoctonia solani isolates collected on potato crops in France. European J. Plant Pathol. 109:983-992 https://doi.org/10.1023/B:EJPP.0000003829.83671.8f
  5. Dhingra O. D., M. L. N. Costa and G. J. Silva Jr (2004) Potential of allyl isothiocyanate to control Rhizoctonia solani seedling damping-off and seedling blight in transplant production. J. Phytopathol. 152:352-357 https://doi.org/10.1111/j.1439-0434.2004.00855.x
  6. Gasztonyi, M. and. H Lyr (1995) Miscellaneous fungicides. pp.390-414, In Modern selective fungicides-properties, applications, mechanisms of action (ed. Lyr, H), Gustav Fischer Verlag, New York
  7. Karadimos D. A., G. S. Karaoglanidis and K. Tzavella-Klonari (2005) Biological activity and physical modes of action of the Qo inhibitor fungicides trifloxystrobin and pyraclostrobin. Crop Protection 24:23-29 https://doi.org/10.1016/j.cropro.2004.06.004
  8. Kataria H R., P. R. Verma and U. Gisi (1991) Variability in the sensitivity of Rhizoctonia solani anastomosis groups to fungicides. J. Phytopathol. 133:121-133 https://doi.org/10.1111/j.1439-0434.1991.tb00145.x
  9. Kuramae E. E., A. L. Buzeto, M. B. Ciampi and N. L. Souza (2003) Identification of Rhizoctonia solani AG 1-I b in lettuce, AG 4 HG-I in tomato and melon, and AG 4 HG-III broccoli and spinach, in Brazil. European J. Plant Pathol. 109:391-395 https://doi.org/10.1023/A:1023591520981
  10. Lewis J. A. and R. D. Lumsden (2001) Biocontrol of damping-off of greenhouse-grown crops caused by Rhizoctonia solani with a formulation of Trichoderma spp. Crop Protection 20:49-56 https://doi.org/10.1016/S0261-2194(00)00052-1
  11. Morita, T, H. Kitajima, T. Higashi and J. Ohta (1988) Variety test and cultivation method for spinach harvested in summer in plastic green house. Bulletin of the Kumamoto Agricultural Experiment Station 13:69-87
  12. Naiki T. and M. Kanoh (1978) Grouping of Rhizoctonia solani Kuhn causing root disease of spinach in plastic house croupping. Ann. Phytopath. Soc. Japan 44:55 4-560 https://doi.org/10.3186/jjphytopath.44.554
  13. Szczech M. and M. Shoda (2004) Biocontrol of Rhizoctonia damping-off of tomato by Bacillus subtilis combined with Burkholderia cepacia. J. Phytopathol. 152: 549-556 https://doi.org/10.1111/j.1439-0434.2004.00894.x
  14. van den Boogert and A. J. G. Luttikholt (2004) Compatible biological and chemical control systems for Rhizoctonia solani in potato. European J. Plant Pathol. 110:111-118 https://doi.org/10.1023/B:EJPP.0000015325.33299.e0
  15. 안재동 (1990) 시금치 주년재배에 관한연구. I 파종기에 따른 수량성. 농시연보 32(3):21-27
  16. 용영록, 정문교, 전지영, 김병섭, 홍세진 (2004) 고랭지 여름재배용 시금치의 품종선발과 재식밀도에 따른 생육 비교, 원예과학기술지 22:283-287
  17. 이주성 (1979) 새로운채소재배기술. 농촌진흥청표준영농교본-41, pp.181-189