The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Effect of temperature and pH on the attachment of Pasteuria penetrans to Meloidogyne arenaria and the mass production

Pasteuria penetrans의 땅콩뿌리혹선층(Meloidogyne arenaria)에 대한 온도와 pH별 부착 및 증식효과

  • Park, Dong-Sik (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Zhu, Yong-Zhe (Dongbu Hannong Chemicals, Yu Song, Science Town) ;
  • Cho, Myoung-Rae (Rural Development Adminstration, National Institute of Highland Agriculture) ;
  • Hur, Jang-Hyun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
  • 박동식 (강원대학교 농업생명과학대학 원예자원생물환경학과군 자원생물환경학과) ;
  • ;
  • 조명래 (농촌진흥청 고령지농업연구소) ;
  • 허장현 (강원대학교 농업생명과학대학 원예자원생물환경학과군 자원생물환경학과) ;
  • 임춘근 (강원대학교 농업생명과학대학 생물자원공학부 응용생물학과)
  • Published : 2005.09.30
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Abstract

The cultivating agroproducts are damaged by the Meloidogyne spp. which are gradually increasing in farm land soil. No effective control method for Meloidogyne spp., however, is available. Pasteuria penetrans which is one of the microorganisms in soil is used for biological control of Meloidogyne spp. although the method of mass production is limited. This study was conducted to investigate attachment and mass production effect of P. penetrans to M. arenaria under different temperatures (10, 30, 50 and $70^{\circ}C$) and pH values (4, 7 and 10). Attachment rates under these temperature and pH were more than 96% and 80%, respectively. In mass production rates, the number of P. penetrans attached on M. arenaria under different temperatures and pH were highly increased in root of tomato but not significantly different. Therefore, we concluded that P. penetrans can survive and attach on M. arenaria under various conditions. This method for mass production of P. penetrans can be provided to develop environmentally-friendly nematicide.

본 연구는 Pasteuria penetrans의 대량 증식조건을 모색하기 위하여 다양한 온도(10, 30, 50, $70^{\circ}C$) 및 pH(4, 7, 10) 조건에서 처리한 P. penetrans의 땅콩뿌리혹선충(Meloidogyne arenaria)에 대한 부착효과와 위 조건에서 부착한 P. penetrans의 증식효과를 알아보았다. 부착 및 증식효과 실험결과 $10{\sim}70^{\circ}C$ 모든 온도 조건에서 96%이상, 모든 pH조건에서 80% 이상 뿌리혹선충에 대한 P. penetrans의 부착율을 보였으며, 상기 모든 온도 및 pH조건에서 부착한 P. penetrans가 토마토 뿌리에서 대량 증식되었으나, 부착 및 증식효과는 온도 및 pH에 영향을 받지 않는 것으로 나타났다. 이러한 결과로부터 얻은 P. penetrans의 대량증식조건은 국내시설재배지에 피해를 주고 있는 뿌리혹선충의 생물학적 방제제 개발을 위한 기초자료가 될 것이라 사료된다.

Keywords

References

  1. Anmed, R. and S. R. Gowen (1991) Studies on the infection of Meloidogyne spp. with isolates of Pasteuria penetrans. Nematologia Mediterranea 19:229-233
  2. Chen, Z. X. and D. W. Dickson (1997) A case study of nematode biocontrol using Pasteuria penetrans. J. Nematol. 29:573
  3. Chen, Z. X. and D. W. Dickson (1998) Review of Pasteuria penetrans: Biology, Ecology, and Biological control potential. J. Nematol. 30:313-340
  4. Chen, Z. X., D. W. Dickson, R McSorley, D. J. Mitchell and T. E. Hewlett (1996) Suppression of Meloidogyne arenaria race 1 by soil application of endospores of Pasteuria penetrans. J. Nematol. 28: 159-168
  5. Cho, M. R, H. Y. Jeon, K. D. Ko, D. S. Kim, S. Y. Na and M. S. Yiem (1997) Screening of oriental melon rootstock cultures for resistance to Meloidogyne incognita. Korea RDA J. Crop Protec. 39:47-51
  6. Cho, M. R., S. Y. Na and M. S. Yiem (2000) Biological control of Meloidogyne arenaria by Pasteuria penetrans. J. Asia-Pacific Entomol. 3(2):71-76 https://doi.org/10.1016/S1226-8615(08)60058-8
  7. Hanna, H. Y, P. D. Colyer, T. L. Kirkpatrick, D. J. Romainem and P. R Cernon (1994) Feasibility of improving cucumber yield without chemical control in soils susceptible to nematode buildup. Hortic. Sci. 29: 1136-1138
  8. Hatz, B. and D. W. Dickson (1992) Effect of temperature on attachment, development, and interactions of Pasteuria penetrans on Meloidogyne arenaria. J. Nematol. 24: 512-521
  9. Huang, J. S. (1985) Mechanism of resistance to root-knot nematodes. pp. 165-174, In An advanced treatise on Meloidogyne, Vol. 1, (ed. Sasser, J. N. and C. C. Carter), North Carolina State University, Graphics, USA
  10. Hussey, R. S. and K. R. Barker (1973) A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis. Rep. 57:1025-1028
  11. Nakasono, K., J. T. T. Gaspard and Y. Tateishi (1993) Effects of soil temperatures on spore increase of Pasteuria penetrans parasitizing Meloidogyne incognita in vinyl house conditions. Japanese J. Nematol. 23:1-9 https://doi.org/10.3725/jjn1993.23.1_1
  12. Oostendorp., M, D. W. Dickson and D. J. Mitchell (1990) Host range and ecology of isolates of Pasteuria spp. from the southeastern United States. J. Nematol. 22: 524-531
  13. Ornat, C., S. Verdejo-Lucas and F. J. Sorribas (1997) Effect of previous crop on population densities of Meloidogyne javanica and yield of cucumber. Nematropica 27:85-90
  14. Sayre, R. M. and M. P. Starr (1985) Pasteuria penetrans(ex Thorne 1940) nom. rev. comb. n., sp. n., a mycelial and endospore forming bacterium parasitic nematodes. Proc. Helminthological Soc. Washington 52:149-165
  15. Stirling, G. R. (1991) Biological control of plant parasitic nematodes. Progress, Problems and Prospects. CAB international, Wallingford, UK
  16. Tzortzakakis, E. A. and S. R Gowen (1994) The evaluation of Pasteuria penetrans alone and in combination with oxamyl, plant resistance and solarization for control of Meloidogyne spp. on vegetables grown in greenhouses of Crete. Crop Protec. 13: 455-462 https://doi.org/10.1016/0261-2194(94)90095-7
  17. Tzortzakakis, E. A., S. Verdejo-Lucas, C. Ornat, F. J. Sorribas and D. E. Goumas (1999) Effect of a previous resistant cultivar and Pasteuria penetrans on population densities of Meloidogyne javanica in greenhouse grown tomatoes in Crete, Greece. Crop Protec. 18:159-162 https://doi.org/10.1016/S0261-2194(98)00106-9
  18. Yu, Y. M., M. R. Cho, Y. Z. Zhu, D. H. Park, J. H. Hur and C. K. Lim (2003) Suppression of Meloidogyne incognita in Lettuce and Oriental Melon by Pasteuria penetrans KW1. Plant Pathol. J. 19(3):177-180 https://doi.org/10.5423/PPJ.2003.19.3.177