Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

GENE EXPRESSION AFTER THE APPLICATION OF THE FLUID-INDUCED SHEAR STRESS ON THE GINGIVAL FIBROBLAST

유체에 의해 유발된 전단력이 치은 섬유아세포 유전자 발현 변화에 미치는 영향에 관한 연구

  • Jeong, Mi-Hyang (Dept. of Orthodontics, College of Dentistry, Seoul National University) ;
  • Choi, Je-Yong (Dept. of Biochemistry, School of Medicine, Kyoungpook National University) ;
  • Chae, Chang-Hoon (Dept. of Oral and Maxillofacial Surgery, School of Medicine, Hallym University) ;
  • Kim, Seong-Gon (Dept. of Oral and Maxillofacial Surgery, School of Medicine, Hallym University) ;
  • Nahm, Dong-Seok (Dept. of Orthodontics, College of Dentistry, Seoul National University)
  • 정미향 (서울대학교 치과대학 치과교정학교실) ;
  • 최제용 (경북대학교 의과대학 생화학교실) ;
  • 채창훈 (한림대학교 의과대학 구강악안면외과) ;
  • 김성곤 (한림대학교 의과대학 구강악안면외과) ;
  • 남동석 (서울대학교 치과대학 치과교정학교실)
  • Published : 2005.09.30
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Abstract

The oral cavity is humid environment mainly due to the continuous salivary flow. The reaction of oral mucosa to fluid flow is important for homeostasis and pathogenesis. The objective of this study is the screening the change of gene expression after the application of fluid induced shear stress (FISS) on the gingival fibroblast using cDNA microarray assay. The immortalized human gingival fibroblasts were grown and FISS was applied using a cone viscometer at a rotational velocity of 40 rpm, respectively for periods of 2 and 4 hours. The synthesis of cDNA was done from the extracted total RNA and cDNA microarray assay was done subsequently. The genes that showed over 1.6 in the Cy3/Cy5 or the Cy5/Cy3 value were regarded as genes influenced significantly by the FISS application ion (/M/>0.7). The " RUNX-1" was increased its expression in 2 hours group and " RUN and SH3 domain containing 1" was increased its expression in 4 hours group. The "CC020415", "cyclin L1", "interferon regulatory factor1", "early growth response 1", "immediate early response 2", and "immediate early response 3" genes were increased their expression in 2 and 4 hours after FISS application. In conclusion, we could find many genes that were probably related to the FISS application. Interestingly, most of them were placed in similar molecular pathways and these findings improve the reliability of chip data and usefulness in overall screening. From this experiment, we could find many items for further study and it will make improvement in the understanding of intracellular events in response to FISS.

Keywords

References

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