Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

THE SHEAR STRESS PROTEOME OF CHONDROCYTES

전단력이 연골세포에 미치는 영향에 관한 연구

  • Kim, Shin-Yeop (Department of Orthodontics, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University) ;
  • Choi, Je-Yong (Department of Biochemistry, School of Medicine, Kyoungpook National University) ;
  • Nahm, Dong-Seok (Department of Orthodontics, School of Dentistry, Seoul National University)
  • 김신엽 (서울대학교 치과대학 치과교정학교실) ;
  • 김성곤 (한림대학교 성심병원 구강악안면외과) ;
  • 최제용 (경북대학교 의과대학 생화학교실) ;
  • 남동석 (서울대학교 치과대학 치과교정학교실)
  • Published : 2005.01.31
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Abstract

The objective of this study is screening the shear stress related proteins in chondrocytes using twodimensional electrophresis and MALDI-TOF. C-28/I2 cell line were grown. The fluid-induced shear stress(FISS) was applied using a cone viscometer at a rotational velocity of 80rpm for periods of 12 hours. Control cultures were tested under identical conditions without mechanical load application. Collected samples were used for the two-dimensional electrophoresis and MALDI-TOF. The identified proteins were calcyclin, RPE-spondin, interleukin-2, extracellular signal regulated kinase (ERK), lamin B2, porA protein, and RET-ELE1 protein. All of them showed a decreased expression. In conclusion, seven proteins were identified as a shear stress related proteins in chondrocytes. As the destruction of articular cartilage is one of main pathogenesis of TMJ internal derangement, this study will give useful information for the understanding of the molecular aspect of TMJ disease.

Keywords

References

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