Maxillofacial Plastic and Reconstructive Surgery

  • 제27권1호
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  • Pages.9-15
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  • 2005
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

전단력이 연골세포에 미치는 영향에 관한 연구

THE SHEAR STRESS PROTEOME OF CHONDROCYTES

  • 김신엽 (서울대학교 치과대학 치과교정학교실) ;
  • 김성곤 (한림대학교 성심병원 구강악안면외과) ;
  • 최제용 (경북대학교 의과대학 생화학교실) ;
  • 남동석 (서울대학교 치과대학 치과교정학교실)
  • Kim, Shin-Yeop (Department of Orthodontics, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University) ;
  • Choi, Je-Yong (Department of Biochemistry, School of Medicine, Kyoungpook National University) ;
  • Nahm, Dong-Seok (Department of Orthodontics, School of Dentistry, Seoul National University)
  • 발행 : 2005.01.31
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초록

The objective of this study is screening the shear stress related proteins in chondrocytes using twodimensional electrophresis and MALDI-TOF. C-28/I2 cell line were grown. The fluid-induced shear stress(FISS) was applied using a cone viscometer at a rotational velocity of 80rpm for periods of 12 hours. Control cultures were tested under identical conditions without mechanical load application. Collected samples were used for the two-dimensional electrophoresis and MALDI-TOF. The identified proteins were calcyclin, RPE-spondin, interleukin-2, extracellular signal regulated kinase (ERK), lamin B2, porA protein, and RET-ELE1 protein. All of them showed a decreased expression. In conclusion, seven proteins were identified as a shear stress related proteins in chondrocytes. As the destruction of articular cartilage is one of main pathogenesis of TMJ internal derangement, this study will give useful information for the understanding of the molecular aspect of TMJ disease.

키워드

참고문헌

  1. Lee JH, Kisiday J, Grodzinsky AJ : Tissue-engineered versus native cartilage: linkage between cellular mechanotransduction and biomechanical properties. Novartis Found Symp 249 : 52, 2003
  2. Almarza AJ, Athanasiou KA : Design characteristics for the tissue engineering of cartilaginous tissues. Ann Biomed Eng 32 : 2, 2004 https://doi.org/10.1023/B:ABME.0000007786.37957.65
  3. Goldring MB, Birkhead JR, Suen LF, et al : Interleukin-1 b-modulated gene expression in immortalized human chondrocytes. J Clin Invest 94 : 2307, 1994 https://doi.org/10.1172/JCI117595
  4. Cawston TE, Curry VA, Summers CA, et al : The role of oncostatin M in animal and human connective tissue collagen turnover and its localization within the rheumatoid joint. Arthritis Rheum 41 : 1760, 1998 https://doi.org/10.1002/1529-0131(199810)41:10<1760::AID-ART8>3.0.CO;2-M
  5. Kaarniranta K, Elo M, Sironen R, et al : Hsp70 accumulation in chondrocytic cells exposed to high continuous hydrostatic pressure coincides with mRNA stabilization rather than transcriptional activation. Proc Natl Acad Sci U S A 95 : 2319, 1998
  6. Labelle Y, Bussieres J, Courjal F, et al : The EWS/TEC fusion protein encoded by the t(9;22) chromosomal translocation in human chondrosarcomas is a highly potent transcriptional activator. Oncogene 18 : 3303, 1999 https://doi.org/10.1038/sj.onc.1202675
  7. Freed AN, Davis MS : Hyperventilation with dry air increases airway surface fluid osmolality in canine peripheral airways. Am J Respir Crit Care Med 159 : 1101, 1999 https://doi.org/10.1164/ajrccm.159.4.9802072
  8. Lee MS, Trindade MC, Ikenoue T et al : Intermittent hydrostatic pressure inhibits shear stress-induced nitric oxide release in human osteoarthritic chondrocytes in vitro. J Rheumatol 30 : 326, 2003
  9. Giard JC, Laplace JM, Rince,A, et al : The stress proteome of Enterococcus faecalis. Electrophoresis 22 : 2947, 2001 https://doi.org/10.1002/1522-2683(200108)22:14<2947::AID-ELPS2947>3.0.CO;2-K
  10. Lopez MF, Melov S : Applied proteomics: mitochondrial proteins and effect in function. Circ Res 90 : 380, 2002 https://doi.org/10.1161/hh0402.105757
  11. Kim SG, Cho BO, Lee YC, et al : Ganglion cyst of the temporomandibular joint. J Oral Pathol Med 32 : 310, 2003 https://doi.org/10.1034/j.1600-0714.2003.00115.x
  12. Lee MS, Trindade MC, Ikenoue T, et al : Intermittent hydrostatic pressure inhibits shear stress-induced nitric oxide release in human osteoarthritic chondrocytes in vitro. J Rheumatol 30 : 326, 2003
  13. Bussolari SR, Dewey CF Jr, Gimbrone MA Jr : Apparatus for subjecting living cells to fluid shear stress. Rev Sci Instrum 53 : 1851, 1982 https://doi.org/10.1063/1.1136909
  14. Schafer BW, Wicki R, Engelkamp D, et al : Isolation of a YAC clone covering a cluster of nine S100 genes on human chromosome 1q21:Rationale for a new nomenclature of the S100 calcium-binding protein family. Genomics 25 : 638, 1993
  15. Courtois-Coutry N, Le Moellic C, Boulkroun S, et al : Calcyclin is an early vasopressin-induced gene in the renal collecting duct: Role in the long-term regulation of ion transport. J Biol Chem 277 : 25728, 2002 https://doi.org/10.1074/jbc.M112435200
  16. Okazaki K, Niki I, Iino S, et al : A role of calcyclin, a $Ca(2^+)$-binding protein, on the Ca(2+)-dependent insulin release from the pancreatic beta cell. J Biol Chem 269 : 6149, 1994
  17. Filipek A, Jastrzebska B, Nowotny M, et al : CacyBP/SIP, a calcyclin and Siah-1 interacting protein, binds EF-hand proteins of the S100 family. J Biol Chem 277 : 21103, 2002 https://doi.org/10.1074/jbc.M111010200
  18. Calabretta B, Battini R, Kaczmarek L, et al : Molecular cloning of the cDNA for a growth factor-inducible gene with strong homology to S-100, a calcium-binding protein. J Biol Chem 261 : 12628, 1986
  19. Kuznicki J, Kordowska J, Puzianowska M, et al : Calcyclin as a marker of human epithelial cells and fibroblasts. Exp Cell Res 200 : 425, 1992 https://doi.org/10.1016/0014-4827(92)90191-A
  20. Breen EC, Fu Z, Normand H : Calcyclin gene expression is increased by mechanical strain in fibro-blasts and lung. Am J Respir Cell Mol Biol 21: 746, 1999 https://doi.org/10.1165/ajrcmb.21.6.3312
  21. Liu M, Post M : Invited review: Mechanochemical signal transduction in the fetal lung. J Appl Physiol 89 : 2078, 2000 https://doi.org/10.1152/jappl.2000.89.5.2078
  22. McCollum R, Martel-Pelletier J, DiBattista J, et al : Regulation of interleukin 1 receptors in human articular chondrocytes. J Rheumatol Suppl. 27 : 85, 1991
  23. Scherle PA, Pratta MA, Feeser WS, et al : The effects of IL-1 on mitogen-activated protein kinases in rabbit articular chondrocytes. Biochem Biophys Res Commun 230 : 573, 1997 https://doi.org/10.1006/bbrc.1996.5985
  24. Hirota Y, Tsukazaki T, Yonekura A, et al : Activation of specific MEK-ERK cascade is necessary for TGF-$\beta$ signaling and crosstalk with PKA and PKC pathways in cultured rat articular chondrocytes.Osteoarthritis Cartilage 8 : 241, 2000 https://doi.org/10.1053/joca.1999.0297
  25. Shakibaei M, Schulze-Tanzil G, de Souza P, et al : Inhibition of mitogen-activated protein kinase kinase induces apoptosis of human chondrocytes. J Biol Chem 276 : 13289, 2001 https://doi.org/10.1074/jbc.M010859200
  26. Hung CT, Henshaw DR, Wang CC, et al : Mitogen-activated protein kinase signaling in bovine articular chondro-cytes in response to fluid flow does not require calcium mobilization.J Biomech 33 : 73, 2000 https://doi.org/10.1016/S0021-9290(99)00176-1
  27. Fanning P, Emkey G, Smith R, et al : Response of cartilage to mechanical loading is correlated with sustained ERK1/2 activation.Trans Orthop Res Soc 26 : 172, 2001
  28. Pearson G, Robinson F, Beers Gibson T, et al : Mitogenactivated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev 22: 153, 2001 https://doi.org/10.1210/er.22.2.153
  29. Yoon YM, Kim SJ, Oh CD, et al : Maintenance of differentiated phenotype of articular chondrocytes by protein kinase C and extracellular signal-regulated protein kinase.J Biol Chem 277 : 8412, 2002 https://doi.org/10.1074/jbc.M110608200
  30. Li WQ, Dehnade F, Zafarullah M : Oncostatin M-induced matrix metalloproteinase and tissue inhibitor of metalloproteinase- 3 genes expression in chondrocytes requires Janus kinase/STAT signaling pathway. J Immunol 166 : 3491, 2001 https://doi.org/10.4049/jimmunol.166.5.3491
  31. Chen TL, Wang PY, Luo W, et al : Aggrecan domains expected to traffic through the exocytic pathway are misdirected to the nucleus experimental cell research 263 : 224, 2001 https://doi.org/10.1006/excr.2000.5093