DOI QR코드

DOI QR Code

Changes of Enzyme Activities and Compositions of Abnormal Fruiting Bodies Grown under Artificial Environmental Conditions in Pleurotus ostreatus

  • Jang, Kab-Yeul (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Cho, Soo-Muk (Agriproduct Science Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • June, Chang-Sung (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Weon, Hang-Yeon (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Park, Jeong-Sik (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Choi, Sun-Gyu (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Cheong, Jong-Chun (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Sung, Jae-Mo (Department of Environmental Biology, Kangwon National University)
  • Published : 2005.03.30

Abstract

This study investigated the biochemical changes of abnormal fruiting bodies grown under artificial environmental conditions in P. ostreatus. Abnormal mushroom growth during cultivation damages the production of good quality mushroom. This study showed that different environmental conditions produced morphological changes in the fruiting bodies of P. ostreatus. The fruiting bodies with morphological changes were collected and examined for differences in biochemical properties, enzyme activities, and carbohydrates composition. The enzyme activities assay showed that glucanase and chitinase activities decreased when the temperature was below or above the optimum cultivation temperature for P. ostreatus. The biochemical compositions of the abnormal mushroom were significantly different from the normal fruiting bodies. It was suggested that the changes in the biochemical composition of abnormal mushroom were caused by the unfavorable environmental conditions during mushroom cultivation.

Keywords

References

  1. Altherthum , F. and Rose , A. H. 1973. Osmotic lysis of spheroplasts from Saccharomyces cerevisia grown anaerobically in media containing different unsaturated fatty acids. J. Gen. Microbiol. 77: 37 1-382 https://doi.org/10.1099/00221287-77-2-371
  2. Atkinson, K. D., Kolat, A. J. and Henry, S. A. 1977. Osmotic imbalance in inositol starved sphaeroplasts of Saccharomyces cerevisiae. J. Bacteriol. 132: 806-807
  3. Cha, D. Y., You, C. H. and Kim, K. P. 1989. New technology of mushroom cultivation. Sangrok press. pp. 1-19
  4. Hamilton, R. J. and Hamilton, S. 1992. Lipid analysis. Oxford University Press. 22-24, 47-48
  5. Harada , A., Gisusi, S., Yoneyarna, S. and Aoyarna, M. 2004. Effects of strain and cultivation medium on the chemical composition of the taste components in fruit body of Hypsizygus marmoreus. Food Chem. 84(2): 265-270 https://doi.org/10.1016/S0308-8146(03)00210-3
  6. Hayashi, E., Hasegawa, R. and Tomita, T. 1978. The fluctuation of various enzyme activities due to myo inositol deficiency in Saccharomyces carlsbergensis. Biochem. Biophys. Acta 540 : 231-237 https://doi.org/10.1016/0304-4165(78)90135-6
  7. Jang, K. Y., Jhune, C. S., Park, J. S., Cho , S. M., Weon, H. Y., Cheong, J. C., Choi , S. G. and Sung, J. M. 2003. Characteriz ation of fruitbod y morphology on various environmental conditions in Pleurotus ostreatus. Mycobiology 31(3): 145-150 https://doi.org/10.4489/MYCO.2003.31.3.145
  8. Jhune, C. S., Jang, K. Y., Kong, W. S., Cho, Y. H., Do, E. S. and Park, S. B. 2003. Additive effect of tobacco waste in substrates on yield and mycelial growth of oyster mushroom, Pleurotus spp. and Trichoderma disease. J. Mushroom Sci. Product. 1(1): 48-53
  9. Jhune, C. S., Kim, G. P. and Shin, C. W. 2000. Effect of rice bran added at spawn-making on the cultivation of oyster mushroom, Pleurotus spp. Kor. J. Mycol. 28(1) :1-5
  10. Jhune, C. S., Kong, W. S., Jang, K. Y., Yoo, Y. B., Do, E. S. and Chun, S. C. 2004. Effect of CaCO, treatment on cultivation of oyster mushroom. J. Mushroom Sci. Product. 2(2): 69-75
  11. Kinukawa, K. and Takarnatsu , Y. 1986. Effect of concentrated carbon dioxide on the fruiting cultivated basidiomycetes (I). Trans. mycol. Soc. Japan 27: 327-340
  12. Kulkarni, R. K. 1990. Mannitol metabolism in Lentinus edodes, the shiitake mushroom. Appl. Environ. Microbiol. 56(1): 250-253
  13. Lowry, O. H., Rosebrou gh, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent . J. BioI. Chem. 193: 265-273
  14. Mansur, M., Arias, M. E., Copa-Patino, J. L., Flardh, M. and Gonzalez, A. E. 2003. The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities. Mycologia 95: 1013-1020 https://doi.org/10.2307/3761909
  15. Ministry of Agriculture & Forestry. 2003. The actual producti on of industrial crop . Pp. 8-9
  16. Oh, S. J., Kim, H. K., Kim, H. K. and Fennor, T. R. 2000. Effect of sodium hypochlorite for controlling bacterial blotch on Pleurotus ostreatus. Mycobiology 28(3): 123-123
  17. Reissig, J. L., Strominger, J. L. and Leloir, L. F. 1955. A modi-fied colorimetric method for the estimation of N-acetylamino sugars. J. BioI. Chem. 217: 959-966
  18. Sanchez, C. 2004. Modem aspects of mushroom culture technology. Appl. Microbiol. Biotechnol. 64: 756-762 https://doi.org/10.1007/s00253-004-1569-7
  19. Somogyi, M. 1952. Notes on sugar determination. J. BioI. Chem. 195: 19-23
  20. Tokimoto, K., Fukuda, M., Kishomoto, H. and Koshitani, H. 1987. Activities of enzymes in bedlogs of Lentinus edodes during body development. Rept. Tottori Mycol. Inst. 25: 24-35
  21. Wakita, S. 1958. Biochemical studies on Collybia velutipes. Part IV. Relation between the growth and the fructification of fungus. J. Agric. Chem. Soc. Jap. 32: 562-566
  22. Wannet, W. J. B., Camp, H. J. M. O., Wisselink, H. W., Drift, C. V. D., Van Griensven, L. J. L. D. and Vogels, G. D. 1998. Purification and characterization of trehalose phosphorylase from the commercial mushroom Agaricus bisporus. Biochimica et Biophysica Acta 1425: 177-188 https://doi.org/10.1016/S0304-4165(98)00066-X