Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Evaluation of Extended Canine Semen by Glass Wool filtration

Glass Wool Filtration을 이용한 개 희석정액의 운동성과 생존률의 검정

  • Yoon Jae-Won (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee Young-Jun (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim Sue-Hee (College of Veterinary Medicine, Chonbuk National University) ;
  • Ji Dong-Beom (Ji Dong Beom Animal Clinic) ;
  • Kim Yong-Jun (College of Veterinary Medicine, Chonbuk National University)
  • Published : 2005.09.01
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Abstract

Damaged spermatozoa are supposed to be trapped in glass wool. In the respect of this, two glass wool filtration spermatozoa groups (0.5 cm, 1 cm depth) were compared with control group to assay sperm motility, HOS values, and vital rate by CFDA/PI staining method following glass wool filtration. The motility of canine sperm extended with PBS+PVP after glass wool filtration was lower in both filtrated groups than that of the control group (p<0.01) and the same significant difference was also shown in canine semen extended with Tris buffer (p<0.01). The motility of canine sperm diluted with PBS+PVP was higher than that diluted with Tris buffer in the same experimental groups (p<0.05). The motility of control group was not significantly decreased until 2 hours immediately after extending, however, the motility of both glass wool filtrated spermatozoa were significantly decreased as time passed until 2 hours after filtration (p<0.01). At each time for assay (immediately, 30 min, 2 hours after filtration), the motility of canine sperm of control group was higher than the filtrated groups (p<0.05), whereas the motility of 0.5 cm depth group was higher than 1 cm depth group at the immediate time after filtration (p<0.05), 30 minutes later (p<0.05) with no difference at 2 hours. No difference was shown among the experimental groups in HOS values of canine sperm after glass wool filtration. The vital rate assayed by CFDA/PI staining of both filter groups was higher than the control group (p<0.05).

Keywords

References

  1. Amann RP. Computerized evaluation of stallion spermatozoa. Proc 33rd Ann Conv Am Ass Equine Pract 1987: 453-473
  2. Anderson EW, Cranwell JE, Pickett BW, Voss JL. The relationship of motility to fertility of frozen stallion spermatozoa. Proc 9th Int Congr Anim Reprod AI, Madrid. 1980; 5: 335-338
  3. Budworth PR, Amann RP, Chapman PL. Relationships between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility. J Androl 1988; 9: 41-54
  4. Clay CM, Slade NP, Amann RP, Squires EL. Effects of extender, storage temperature and centrifugation on stallion spermatozoal motility and fertility. Proc 10th Int Congr Anim Reprod & AI, Urbana. 1984; 4: 186-189
  5. Crabo BG, Pavelko MK, Kosco MS, Lohdi IA, Loseth KJ. Evaluation of frozen boar semen by surgical insemination, sperm motility, acrosome damage and Sephadex filtration. Proc 10th Int Congr Anim Reprod AI 1984; 2: No. 59
  6. Crabo BG, Loseth KJ, Weidel L. Trapping of morphological types of bull spermatozoa by Sephadex/glass wool filter. Proc 12th Int Congr Anim Reprod AI, The Hague. 1992; 1: 423-425
  7. Deibel GC, Smith JE, Crabo BG, Graham EF. Evaluation of six assays of sperm quality by means of their accuracy, precision and sensitivity in separating known levels of damage. Proc 8th Int Congr Anim Reprod AI 1976; 4: 888-891
  8. Engel S, Weber H, Petzoldt R, Seidle B, Wiehe W, Slperl J. An improved method of sperm selection by glass wool filtration. Andrologia 2001; 33: 223-230 https://doi.org/10.1046/j.1439-0272.2001.00434.x
  9. Fayemi EO, Crabo BG, Graham EF. Assay of frozen boar semen with Sephadex filtration. Theriogenology 1979; 12: 13-17 https://doi.org/10.1016/0093-691X(79)90053-0
  10. Graham EF, Vasquez IA, Schmehl MK, Evensen BK. An assay of semen quality by use of Sephadex filtration. Proc 8th Int Congr Anim Reprod & AI, Cracow. 1976; 4: 896-899
  11. Graham EF, Schmehl MK, Evensen BK. An Overview of column separation of spermatozoa. Proc 7th Techn Conf NAAB Reprod AI 1978: 69-73
  12. Graham EF, Schmehl MK, Nelson DS. Problems with laboratory assays. Proc 8th Tech Conf NAAB Reprod AI 1980: 59-66
  13. Heuer C. Versuche Zur Tiefgefrierkonservierung von Wasserb?ffelsperma unter Anvendung des Filtertests zur Samenbeurteilung. Inaug Diss Vet Med Hannover. 1980
  14. Heuer C, Tahir-Nazir M, Crabo BG, Bader H, Shah M, Saji MA. Simple method for the assay of water buffalo semen by filtration through sephadex. Pakistan Vet J 1983; 3: 157-161
  15. Jasko DJ, Lein DL, Foote RH. Determination of the relationship between morphologic classifications and fertility in stallion: 66 cases (1987-1988). J Am Vet Med Assn 1990; 197: 389-394
  16. Kissinger CH, Skinner MK, Griswold MD. Analysis of Sertoli cell-secreted proteins by two-dimensional gel electrophoresis. Biol Reprod 1982; 27: 233-240 https://doi.org/10.1095/biolreprod27.1.233
  17. Landa CA, Almquist JO, Amman RP. Factors influencing Sephadex separation of bovine and ovine spermatozoa. J Dairy Sci 1980; 63: 277-282 https://doi.org/10.3168/jds.S0022-0302(80)82925-0
  18. Lodhi LA, Crabo BG Filtration of bull spermatozoa through Sephadex, polyacrylamide, silica gel and glass wool in the presence and absence of two sugars. Proc 10th Int Congr Anim Reprod & AI, Urbana. 1984; 2: 59-61
  19. Loseth KJ, Wolff K, Hamilton DW, Crabo BG Trapping of in vitro capacitated boar spermatozoa in Sephadex and glass wool filters. Proc 12th Int Congr Anim Reprod AI, The Hague. 1992; 3: 1575-1577
  20. M?ller Z. Practicalities of insemination of mares with deep-frozen semen.J Dairy Sci 1987; 35: 121-125
  21. Obonyo M, Loseth KJ, Crabo BG. Relation between the fertility of frozen boar semen and semen quality measured as sperm motility and with glass wool/Sephadex filters. Proc 12th Int Congr Anim Reprod AI, The Hague. 1992; 1: 505-507
  22. O'Connor MT, Amann RP, Saacke RG. Comparisons of computer evaluations of spermatozoal motility with standard laboratory tests and their use for predicting fertility. J Anim Sci 1981; 53: 1368-1376
  23. Pickett BW, Sullivan JJ, Byers WW, Pace MM, Remmenga EE. Effect of centrifugation and seminal plasma on motility and fertility of stallion and bull spermaozoa. Fertil Steril 1975; 26: 167-174
  24. Rosner B. Fundamentals of Biostatistics. Ed B Rosner Duxbury Press Boston MA. 1982: 225-452
  25. Saacke RC, White JM. Semen quality and their relationship to fertility. Proc 4th Tech Conf NAAB AI Reprod 1972: 22-27
  26. Samper JC, Crabo BG, Behnke EJ, Byers AP, Hunter AG. In vitro capacitation of stallion semen and its filterability through filters containing Sephadex. Proc Conf Res Wor Anim Dis, Chicago. 1986: 236(Abstr)
  27. Samper JC, Crabo BG. Filtration of capacitated stallion spermatozoa through filters containing glass wool and/or Sephadex. Proc 11th Int Congr Anim Reprod & AI, Dublin. 1988; 3: 295
  28. Samper JC, Loseth KJ, Crabo BG. Evaluation of horse spermatozoa with Sephadex filtration using three extenders and three dilutions. Proc 11th Int Congr Anim Reprod AI, Dublin. 1988; 3: 294
  29. Samper JC, Behnke EJ, Byers AP, Hunter AG, Brabo BG. In Vitro capacitation of stallion spermatozoa in calcium-free medium and penetration of zona free hamster eggs. Theriogenology 1989; 31: 875-884 https://doi.org/10.1016/0093-691X(89)90032-0
  30. Samper JC. Evaluation of Membrane Integrity of Preserved Stallion Spermaozoa. PhD Dissertaion, University of Minnesota. 1990
  31. Samper JC, Crabo BG, Hamilton DW. A clusterin-like protein in stallion sperm and reproductive fluids binds to Sephadex. J Androl Suppl 1990; 11: 26-P
  32. Samper JC, Hamilton DW, Crabo BG. A clusterin-like protein in stallion sperm and reproductive fluids binds to Sephadex. Proc 15th Ann Mtg American Society of Andrology 1990: 26
  33. Samper JC, Hellander JC, Crabo BG. Relationship between the fertility of fresh and frozen stallion semen and semen quality. J Reprod Fert Suppl 1991; 44: 107-114
  34. Samper JC, Crabo BG. Assay of capacitated, freeze-damaged and extended stallion spermatozoa by filtration. Theriogenology 1993; 39: 1209-1220 https://doi.org/10.1016/0093-691X(93)90224-S
  35. Samper JC, Hamiltion DW, Pryor JL, Loseth KJ, Troedsson MHT, Crabo BG. Mechanism of Sephadex Trapping of Capacitated Stallion Spermatozoa. Biol Reprod Mono 1995; 1: 729-737
  36. Yanagimachi R. Zona-free hamster eggs: Their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa. Gamete Res 1984; 5: 323-344 https://doi.org/10.1002/mrd.1120050404