Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Controlled Release of Cefadroxil from Chitosan Beads in Dogs

개에서 키토산 비드를 이용한 cefadroxil 방출제어

  • Kim Dae-Keun (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Park Seung-Chun (Department of Veterinary Pharmacology, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim Tae-Wan (Department of Veterinary Physiology, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee Keun-Woo (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Oh Tae-Ho (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University)
  • 김대근 (경북대학교 수의과대학 내과학교실) ;
  • 박승춘 (경북대학교 수의과대학 약리학교실) ;
  • 김태완 (경북대학교 수의과대학 생리학교실) ;
  • 이근우 (경북대학교 수의과대학 내과학교실) ;
  • 오태호 (경북대학교 수의과대학 내과학교실)
  • Published : 2005.09.01
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Abstract

The purpose of this study is to investigate the effects of formulation variables on the release of cefadroxil form chitosan beads, to optimize the preparation of chitosan beads loaded with the drug for controlled release, and to evaluate the drug release form chitosan beads in dogs. Chitosan beads were prepared with tripolyphosphate (TPP) by ionic cross-linking and those sizes were less than 1 mm in diameter. The release behaviour of cefadroxil was affected various factors. As pH of TPP solutions decreased, the entrapment efficiency of cefadroxil increased, whereas the release of cefadroxil decreased. The release rate of cefadroxil form chitosan beads decreaed with the increased TPP solution concentration. When cross-linking time increased, the release of the drug from chitosan beads decreased. The cefadroxil loaded beads were implanted to 4 mixed breed dogs. The concentration of cefadroxil in sera due to chitosan beads implanted with 50 mg/kg body weight of beads was sustained more than 1 ug/ml for the whole 7 days period. Therefore, the cefadroxil loaded beads can be used successfully in pyoderma of dogs. These results indicate that chitosan beads may become a potential delivery system to control the release of drug.

본 연구는 개에서 키토산 비드를 이용한 cefadroxil 방출에 영향을 주는 인자, 약물을 함유한 최적의 키토산 비드의 제조, 그리고 키토산 비드로부터 약물의 방출을 평가하는 것이다. 키토산 비드는 tripolyphosphate (TPP)와 이온결합으로 생성되며 비드의 크기는 1 mm 미만이었다. 비드로부터 cefadroxil 방출은 여러 인자에 영항을 받는다. TPP의 pH가 감소할수록 cefadroxil의 비드내 함유량은 증가하지만, 비드로부터 방출량은 감소한다. Cefadroxil의 방출속도은 TPP 농도가 증가할수록 감소한다. 결합시간이 길어지면, 방출량이 감소한다. Cefadroxil을 함유한 키토산 비드를 50 mg/kg 용량으로 건강한 개 4두의 피하에 이식한 결과, cefadroxil의 혈청내 농도는 1 ${\mu}g/ml$ 이상으로 7일간 유지되었다. 따라서 cefadroxil을 함유한 키토산 비드는 개의 농피증 치료에 유용한 것으로 사료되며 약물방출을 통제할 수 있는 약물수송체가 이용될 수 있다고 사료된다.

Keywords

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