Methicillin 내성 S. aureus 임상분리균주의 Coagulase와 주요 독소 유전자의 PCR 검출

PCR Detection of Virulence Genes Encoding Coagulase and Other Toxins among Clinical Methicillin-Resistant Staphylococcus aureus Isolates

  • 정혜진 (중앙대학교 식품공학과) ;
  • 조준일 (중앙대학교 식품공학과) ;
  • 송은섭 (인하대학교 의과대학 산부인과학교실) ;
  • 김진주 (인하대학교 의과대학 진단검사의학과) ;
  • 김근성 (중앙대학교 식품공학과)
  • Jung Hye-Jin (Department of Food Science and Technology, Chung-Ang University) ;
  • Cho Joon-Il (Department of Food Science and Technology, Chung-Ang University) ;
  • Song Eun-Seop (Department of Obstetrics and Gynecology, College of Medicine, Inha University) ;
  • Kim Jin-Ju (Department of Laboratory Medicine, College of Medicine, Inha University) ;
  • Kim Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
  • 발행 : 2005.09.01

초록

본 연구에서는 임상에서 분리한 MRSA 균주(n=49)를 대상으로 이 균의 병원성과 관계가 있는 것으로 알려진 유전자들을 선정하여 PCR 방법을 이용하여 이들 유전자들의 보유 유무를 결정하였다. 이들 MRSA 균주는 모두 coa 유전자를 보유하고 있었고, 또한 이들 유전자는 500bp($6{\%}$), 580bp($27{\%}$), 660bp($65{\%}$) 및 740bp($2{\%}$)로 4가지 종류의 polymorphism이 검출되었다. Hemolysin 유전자의 경우 4-5종 이상 다른 locus들을 보유하였고, 그 중 25개 균주($51{\%}$)가 hla / hlb / hld / hlg / hig-2 유전자를 모두 보유하였으며, 가장 많은 분포를 나타내었다. 한편, MRSA 균주는 다양한 enterotoxin 유전자의 조합을 보였으며, sea와 seb 유전자의 경우 모든 49개 균주에서 보유하고 있었다. 그러나 sei 유전자는 31균주($63{\%}$), tsst-1 유전자는 16균주($33{\%}$), seg 유전자는 14균주($29{\%}$), sec 유전자는 8균주($16{\%}$), seh 유전자는 5균주($10{\%}$), sed 유전자와 sej 유전자는 1균주($2{\%}$)에서 각각 검출되었다. 그러나 see 유전자 및 eta와 etb유전자는 어떤 분리균주에서도 검출되지 않았다. 또한 sea / seb 유전자 조합이 11개 균주($23{\%}$)로부터, sea / seb / sei 유전자 조합은 9개 균주($19{\%}$)로부터, sea / seb / seg / sei /tsst-1 유전자 조합은 5개 균주($10{\%}$)로부터 각각 검출되었다. 그리고 다른 유전자의 조합은 $10{\%}$이하로 검출되었다.

To characterize the genotypic traits of clinical methicillin-resistant Staphyiococus aureus (MRSA) isolates (n=49), major virulence-associated genes were detected by using PCR-based methods. All the MRSA isolates possessed coagulase gene and showed four polymorphism types [500bp ($6{\%}$),580bp ($27{\%}$), 660bp ($65{\%}$) and 740bp ($2{\%}$)] due to variable numbers of tandem repeats present within the gene. The four or five different loci of hemolysin gene family were dominant in the MRSA isolates,25 of which($51{\%}$) possessed a combination of hla / hlb / hld/ hlg / hlg-2 genes as the most prevalent type. The prevalence of enterotoxin genes was varied among the MRSA isolates. sea and seb genes were detected from all the MRSA isolates. But sei, tsst-1, seg, sec, and seh genes were detected from 31 ($63{\%}$), 16 ($33{\%}$), 14 ($29{\%}$), 8 ($16{\%}$), and 5 ($10{\%}$) isolates, respectively. sed and sej genes were detected from 1 ($2{\%}$) isolate, respectively. see, eta, and etb genes were not detected at all. sea / seb genes were co-detected from 11 ($23{\%}$) isolates, sea / seb / sei genes from 9 ($19{\%}$) isolates, and sea / seb / seg / sei / tsst-1 genes from 5 ($10{\%}$) isolates. Other genes were co-detected with below $10{\%}$ frequencies.

키워드

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