Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Bacillus sp. Producing Poly-$\gamma$-glutamic Acid with High Efficiency and Its Characterization

고효율 Poly-$\gamma$-Glutamic Acid생산 균주의 분리 및 생산 특성

  • You Kyung-Ok (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Oh You-Na (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim Byung-Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Nam Soo-Wan (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Jeon Sung-Jong (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kim Dong-Eun (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kim Young-Man (Department of Food and Nutrition/Oriental Biotech. Co., Dong-Eui University) ;
  • Kwon Hyun-Ju (Department of Life Science and Biotechnology, Dong-Eui University)
  • 유경옥 (동의대학교 생명응용과학과) ;
  • 오유나 (동의대학교 생명응용과학과) ;
  • 김병우 (동의대학교 생명응용과학과) ;
  • 남수완 (동의대학교 생명공학과) ;
  • 전숭종 (동의대학교 생명공학과) ;
  • 김동은 (동의대학교 생명공학과) ;
  • 김영만 (동의대학교 식품영양학과 및 ㈜오리엔탈바이오텍) ;
  • 권현주 (동의대학교 생명응용과학과)
  • Published : 2005.09.01
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Abstract

A bacterium with high productivity of poly-$\gamma$-glutamic acid (PGA) was isolated from the traditional Korean seasoning, ChungKookJang. The 16s ribosomal RNA sequence of isolated strain showed 97.6, 98.9 and $90.3{\%}$ of similarity to those of Bacillus sp. WL-3, Bacillus subtilis; ENV1 and B amy-loliquefaciens (T), respectively. Accordingly, this bacterium was identified as a Bacillus sp. However, some biochemical characteristics of this strain were different from those of B. subtilis: D-xylose fermentation and glycogen utility were negative. Maximum production of PGA was achieved when it was grown aerobically in a culture medium containing glutamic acid ($3{\%}$) and fructose ($4{\%}$) as carbon sources. The volumetric yield of PGA reached up to 27 g/l in the optimum culture medium. These results suggest that the present strain can be applicable for industrial purposes such as a prototype strain for food or cosmetics industry.

한국 청국장에서 poly-$\gamma$-glutamic acid (PGA)를 대량 생산하는 세균을 분리하였다. 이 세균의 16s ribosomal RNA 서열을 분석한 결과 Bacillus subtilis BFAS, B. subtilis MO4와 B. amyloliquefaciens B128과 99.0, 97.7 그리고 $97.3{\%}$의 상동성을 각각 나타내었다. 따라서 본 분리 균주를 Bacillus sp.로 동정하고 Bacillus sp. YN-1로 명명하였다. PGA 대량생산 을 위해 생산 조건을 검토한 결과 $3{\%}$ glutamic acid, $4{\%}$ fructose를 탄소원으로 첨가하였을 때 최대량의 PGA를 생산하는 것을 알 수 있었다. 또한 PGA 최대 생산량은 최적 배양 조건에서 27 g/l의 양으로 생산되어 본 균주는 PGA 대량 생산에 적합한 세균임을 확인할 수 있었으며 식품 및 화장품 산업에 유용하게 사용할 수 있을 것으로 사료된다.

Keywords

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